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Influence of arbuscular mycorrhizae on heavy metal (Zn and Pb) uptake and growth of Lygeum spartum and Anthyllis cytisoides

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Abstract

The influence of arbuscular mycorrhizae (AM) on plant growth and Zn and Pb uptake by Lygeum spartum and Anthyllis cytisoides was studied in soils with different levels of these heavy metals. A. cytisoides is highly dependent on AM for optimal growth, while L. spartum is a facultative mycotroph.

Mycorrhizal and nonmycorrhizal plants were grown in soil supplemented with 0, 10, 100 and 1000 mg of Zn kg-1 soil or 0, 100 and 1000 mg of Pb kg-1 soil. Two different mycorrhizal fungi were separately studied: Glomus macrocarpum isolated from a non contaminated site and a strain of Glomus mosseae isolated from a soil contaminated with these metals. The infectivity of the fungi was not affected by the presence of Zn or Pb in the soil. In unamended soil, both fungi were equally effective in promoting plant growth, but when Zn or Pb were added to soils, G. mosseae was more efficient than G. macrocarpum in stimulating plant growth of A. cytisoides. A. cytisoides was unable to grow unless mycorrhizal. Metal addition to the soil induced a reduction in the biomass of L. spartum and of mycorrhizal A. cytisoides, and a decrease in shoot P concentration of mycorrhizal plants. The concentration of metals in the plants varied according to the amount added to the soil and to the inoculation treatment: at low doses, mycorrhizal plants showed equal or higher concentration of Zn or Pb than nonmycorrhizal ones; at higher doses, however, metal concentrations in the plants inoculated with G. mosseae were lower than those found in the corresponding controls, while the plants inoculated with G. macrocarpum showed similar (L. spartum) or even higher (A. cytisoides) levels than the controls.

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Authors and Affiliations

  1. Departamento de Biología Vegetal, Facultad de Biología, Universidad de Murcia, Spain

    G. Díaz & M. Honrubia

  2. Departamento de Microbiología, Estación Experimental del Zaidín, CSIC, Granada, Spain

    C. Azcón-Aguilar

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  1. G. Díaz
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  2. C. Azcón-Aguilar
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  3. M. Honrubia
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Díaz, G., Azcón-Aguilar, C. & Honrubia, M. Influence of arbuscular mycorrhizae on heavy metal (Zn and Pb) uptake and growth of Lygeum spartum and Anthyllis cytisoides . Plant Soil 180, 241–249 (1996). https://doi.org/10.1007/BF00015307

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  • DOI: https://doi.org/10.1007/BF00015307

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