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Transformation of Bacillus subtilis by electroporation

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Biotechnology Techniques

Summary

Optimal conditions for the transformation of Bacillus subtilis by electroporation were achieved. Frequencies of greater than 105 transformants/μg of plasmid DNA were obtained for a number of strains and plasmids. Increased transformation efficiency of mini-prep DNA from B. subtilis and Escherichia coli was obtained after microdialysis.

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Authors and Affiliations

  1. Institute of Horticultural Research, BN17 6LP, Littlehampton, West Sussex, UK

    Michele Stephenson & Paul Jarrett

Authors
  1. Michele Stephenson
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  2. Paul Jarrett
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Stephenson, M., Jarrett, P. Transformation of Bacillus subtilis by electroporation. Biotechnol Tech 5, 9–12 (1991). https://doi.org/10.1007/BF00152746

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  • DOI: https://doi.org/10.1007/BF00152746

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