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Inoculation and tissue distribution in pilot-scale plant root culture bioreactors

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Biotechnology Techniques

Summary

Inoculation of large-scale plant root culture reactors can be carried out by briefly homogenizing bulk root tissue, followed by aseptic transfer as a slurry to the reactor. Uniform root distribution can be achieved in bioreactors by entrapment of the growing root inoculum onto process packing elements (e.g. distillation packings), randomly distributed within the reactor, in a bubble column operation. These two techniques have been successfully used to inoculate a 14 L pilot-scale reactor which is subsequently operated as a trickle bed reactor.

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Authors and Affiliations

  1. Department of Chemical Engineering, The Pennsylvania State University, 16802, University Park, PA, USA

    Divakar Ramakrishnan, John Salim & Wayne R. Curtis

  2. The Biotechnology Institute The Pennsylvania State University, 16802, University Park, PA, USA

    Wayne R. Curtis

  3. 111 Fenske Laboratory, Dept. of Chemical Engineering, PSU, 16802, University Park, PA, USA

    Wayne R. Curtis

Authors
  1. Divakar Ramakrishnan
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  2. John Salim
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  3. Wayne R. Curtis
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Ramakrishnan, D., Salim, J. & Curtis, W.R. Inoculation and tissue distribution in pilot-scale plant root culture bioreactors. Biotechnol Tech 8, 639–644 (1994). https://doi.org/10.1007/BF00241690

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  • DOI: https://doi.org/10.1007/BF00241690

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