Summary
Imaginal discs ofDrosophila melanogaster larvae, 24–53 hrs after oviposition, were transplanted into mature immobile larval hosts. The transplants did not respond to the hormonal stimuli of metamorphosis, but instead completed their larval development. When reinjected into mature larval hosts, they now differentiated the full set of their presumptive imaginal structures. The process of acquiring competence for metamorphosis appears to be independent of the hormonal conditions.
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References
Bodenstein, D.: Untersuchungen zur Metamorphosenproblem. I. Kombinierte Schnürungs- und Transplantationsexperimente anDrosophila. Wilhelm Roux' Arch. Entwickl.-Mech. Org.137, 474–505 (1938).
Bodenstein, D.: Investigations on the problem of metamorphosis. IV. Developmental relations of interspecific organ transplants inDrosophila. J. exp. Zool.82, 1–30 (1939).
Bodenstein, D.: Hormones and tissue competence in the development ofDrogophila. Biol. Bull.84, 34–59 (1943).
Ephrussi, B., Beadle, G. W.: A technique of transplantation forDrosophila. Amer. Naturalist70, 218–225 (1936).
Gehring, W., Nöthiger, R.: The imaginal discs ofDrosophila. In: Developmental systems. — Insects (C. H. Waddington and S. J. Counce, eds.). New York-London: Academic Press 1972.
Ginter, E. K., Kuzin, B. A.: Readiness of eye and antennal imaginal discs inDrosophila melanogaster of different instars for differentiation. Ontogenese [Russian paper]1, 492–500 (1970).
Hadorn, E.: Konstanz, Wechsel und Typus der Determination und Differenzierung in Zellen aus männlichen Genitalanlagen vonDrosophila melanogaster nach Dauerkulturin vivo. Develop. Biol.13, 424–509 (1966).
Hadorn, E., Neel, J.: Der hormonale Einfluß der Ringdrüse (Corpus allatum) auf die Pupariumbildung bei Fliegen. Wilhelm Roux' Arch. Entwickl.-Mech. Org.138, 281–304 (1938).
Lindsley, D. L., Grell, E. H.: Genetic variations ofDrosophila melanogaster. Carnegie Inst. Wash. Publ. No627 (1968).
Mandaron, P.: Développementin vitro des disques de laDrosophile. Aspects morphologiques et histologiques. Develop. Biol.22, 298–320 (1970).
Mandaron, P.: Sur le mécanisme de l'évagination des disques imaginaux chez laDrosophile. Develop. Biol.25, 581–605 (1971).
Melander, Y., Wingstrand, K. G.: Gömöri's hematoxylin as a chromosome stain. Stain Technol.28 (5), 217–223 (1953).
Nöthiger, R., Oberlander, H.: Differentiation of pulsating regions in genital imaginal discs after culturein vivo (Drosophila melanogaster). J. exp. Zool.164, 61–68 (1967).
Oberlander, H.: Effects of eedysone, ecdysterone and inokosterone on thein vitro initiation of metamorphosis of wing discs ofGalleria mellonella (L.). J. Insect Physiol.15, 297–304 (1969).
Postlethwait, J. H., Schneiderman, H. A.: Induction of metamorphosis by ecdysone analogues:Drosophila imaginal discs culturedin vivo. Biol. Bull.138, 47–55 (1970).
Schneider, I.: Insect tissue culture. In: Methods in developmental biology (ed. by F. H. Wilt and N. K. Wessells). New York: Thomas Y. Crowell Co. 1967.
Sengel, P., Mandaron, P.: Aspects morphologiques du développementin vitro des disques imaginaux de laDrosophile. C. R. Acad. Sci. (Paris)268, 405–407 (1969).
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Supported by a credit of the Swiss National Foundation granted to Prof. Dr. E. Hadorn. I thank Dr. R. Nöthiger for his valuable criticism during this investigation.
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Mindek, G. Metamorphosis of imaginal discs of Drosophila melanogaster. W. Roux' Archiv f. Entwicklungsmechanik 169, 353–356 (1972). https://doi.org/10.1007/BF00580256
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DOI: https://doi.org/10.1007/BF00580256