Summary
Using microquantitative measurements of alcohol dehydrogenase activity in microdissected samples of liver tissue along the sinusoidal length, the intra-acinar distribution profiles were studied in seven groups of female rats at different times during 24h with a light phase from 6∶30h to 18∶30h. The mean values of alcohol dehydrogenase activity showed a circadian rhythm with a minimum at 13.30h and a maximum at 17.30h (p<0.0001). However, the intra-acinar gradients remained almost unchanged, indicating that increase and decrease in enzyme activity takes place simultaneously in all parts of the liver acinus. This observation, together with data from the literature, suggests that the circadian rhythm of alcohol dehydrogenase activity reflects variations in different liver cell consituents, rather than enzyme protein synthesis or proteolysis.
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References
Deimling, M. J. &Schnell, R. C. (1980) Circadian rhythms in the biological response and disposition of ethanol in the mouse.J. Pharmacol. Exptl. Ther. 213, 1–8.
Guerri, C., Wallace, R. &Grisolia, S. (1978) The influence of prolonged ethanol intake on the levels and turnover of alcohol and aldehyde dehydrogenases and of brain (Na++K+)-ATPase of rats.Eur. J. Biochem. 86, 581–7.
Halberg, F. (1959) Physiologic 24-hour periodicity; general and procedural considerations with reference to the adrenal cycle.Z. Vitamin-, Horm.-Fermentforsch. 10, 252–5.
Jungermann, K., Katz, N., Teutsch, H. &Sasse, D. (1977) Fossible metabolic zonation of liver parenchyma into glucogenic and glycolytic hepatocytes. InAlcohol and aldehyde metabolizing systems. (edited byThurman, R., Williamson, J., Drott, H. &Chance, B.) New York: Academic Press.
Lardeux, B., Bourdel, G. &Girard-Globa, A. (1978) Regulation of hepatic synthesis of proteins by the chronology of protein ingestion.Biochem. Biophys. Acta 518, 113–24.
Lowry, O. H. &Passonneau, J. V. (1972)A flexible system of enzymatic analysis. New York, London: Academic Press.
Maly, I. P. &Sasse, D. (1983) A technical note on the histochemical demonstration of G6Pase activity.Histochem. 78, 409–11.
Maly, L. P. &Sasse, D. (1985) Microquantitative determination of the distribution patterns of alcohol dehydrogenase activity in the liver of rat, guinea-pig and horse.Histochem. 83, 431–6.
Maly, I. P. &Sasse, D. (1987a) The intra-acinar distribution patterns of alcohol-dehydrogenase activity in the liver of juvenile, castrated and testosterone-treated rats.Biol. Chem. Hoppe-Seyler 368, 315–21.
Maly, I. P. &Sasse, D. (1987b) The effects of starving and refeeding on the intra-acinar distribution pattern of alcohol-dehydrogenase activity in rat liver.Histochem. 86, 275–9.
Mayersbach, H. V. (1981) Chronobiological aspects in histochemistry. InHandbuch der Histochemie 1, Allg. Methodik 4, (edited byGraumann, W. &Neumann, K.). Stuttgart, New York: Fischer-Verlag.
North, C., Feubrs, R. J., Scheving, L. E., Pauly, J. E., Tsai, T. H. &Casciano, D. A. (1981) Circadian organization of thirteen liver and six brain enzymes of the mouse.Am. J. Anat. 162, 183–99.
Rognstad, R. &Grunnet, N. (1979) Enzymatic pathways of ethanol metabolism. InBiochemistry and pharmacology of ethanol Vol. 1/4 (edited byMajchrowicz, E. &Noble, E.). New York and London: Plenum Press.
Scheving, L. E., Dunn, J. D., Pauly, J. E. &Harrison, W. H. (1972) Circadian variation in rat serum 5-hydroxytryptamine and effects of stimuli on the rhythm.Amer. J. Physiol. 222, 252–5.
Sturtevant, R. P. &Garber, S. L. (1981) Meal timing dominates the lighting regimen as a synchronizer of the circadian blood ethanol clearance rate rhythm in rats.J. Nutr. 111, 2000–5.
Sturtevant, R. P. &Garber, S. L. (1984) Circadian rhythms of alcohol dehydrogenase and MEOS in the rat.Proc. Soc. Exptl. Biol. Med. 175, 299–303.
Sturtevant, R. P. &Garber, S. I. (1988) Chronopharmacology of ethanol: acute and chronic administration in the rat.Ann. Rev. Chronopharmacol. 4, 47–57.
Sturtevant, F. M. &Sturtevant, R. P. (1979) Chronopharmacokinetics of ethanol. InBiochemistry and pharmacology of ethanol Vol. 1/2 (edited byMajchowicz, E. &Noble, E. P.). New York and London: Plenum Press.
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Salsano, F., Maly, I.P. & Sasse, D. The circadian rhythm of intra-acinar profiles of alcohol dehydrogenase activity in rat liver: a microquantitative study. Histochem J 22, 395–400 (1990). https://doi.org/10.1007/BF01003458
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DOI: https://doi.org/10.1007/BF01003458