Abstract
A mouse myeloma cell line growing in suspension was subjected intermittently to flow through a sudden contraction and turbulent flow in a capillary tube. The probability of lysis per pass through the capillary tube increased with average wall shear stress level and with residence time per pass in the tube. Lysis was first observed at a threshold average wall shear stress level of 1800 dyn/cm2. Although the flow caused lysis, it had no effect on cell viability.
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References
Augenstein, D.C., Sinskey, A.J., and Wang, D.I.C. (1971).Biotechnol. Bioeng. 8, 409–418.
Bentley, B.J., and Leal, L.G. (1986).J. Fluid Mech. 167, 241–284.
Cherry, R.S., and Papoutsakis, E.T. (1987).Biotechnol. Bioeng. (in press).
Croughan, M.S., Hamel, J.-F., and Wang, D.I.C. (1987).Biotechnol. Bioeng. 29, 130–141.
Davies, J.T. (1972).Turbulence Phenomena New York: Academic Press.
Davies, P.F., Remuzzi, A., Gordon, E.J., Dewey, C.F., Jr., and Gimbrone, M.A. (1986).PNAS (USA) 83, 2114–2117.
Han, C.D., and Funatsu, K. (1978).J. Rheology 22, 113–133.
Hinze, J.O. (1959).Turbulence New York: McGraw-Hill.
Meilhoc, E., Moulin, M.J., and Osborne, H.B. (1987).J. Cell. Physio. 131, 465–471.
Rhee, B.-G., and McIntire, C.V. (1986).Chem. Eng. Commun. 47, 147–161.
Smith, C.G., Greenfield, P.F., and Randerson, D.H. (1987).Biotech. Techniques 1, 39–41.
Stathopoulos, N.A., and Hellums, J.D. (1985).Biotechnol. Bioeng. 27, 1021–1026.
Tramper, J., Williams, J.B., and Joustra, D. (1986).Enzyme Microb. Technol. 8, 33–36.
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McQueen, A., Meilhoc, E. & Bailey, J.E. Flow effects on the viability and lysis of suspended mammalian cells. Biotechnol Lett 9, 831–836 (1987). https://doi.org/10.1007/BF01026191
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DOI: https://doi.org/10.1007/BF01026191