Summary
The kinetic parame6ers for soluble (Km=7.4mM and, Vmax=2.6 U/mL) and cell wall bound invertase (Km=15.4mM and Vmax=3.2 U/L) were determined. The invertase activity for both forms varied by around 10% against sucrose concentration ranging from 80 g/L to 200 g/L, whereas the transferase activity increased markedly with increasing sucrose concentration.
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Vitolo, M., Yassuda, M.T. Effect of sucrose concentration on the invertase activity of intact yeast cells (S. cerevisiae). Biotechnol Lett 13, 53–56 (1991). https://doi.org/10.1007/BF01033517
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DOI: https://doi.org/10.1007/BF01033517