Abstract
It is postulated that ovarian hyperstimulation prior to embryo transfer results in changes in the endometrium which lead to poor adhesive properties of the endometrium. Based on the properties of fibrin sealant which is used in human operative medicine, it is believed that adhesion of embryos to the endometrium during embryo transfer may be encouraged by the addition of fibrin sealant. The purpose of this study was to assess the effects of the addition of fibrin sealant to a mouse embryol Ham's F-10 culture medium mixture. There was no statistical difference in the number of blastocysts developing from 138 embryos in standard medium compared with 139 cultured with the addition of fibrin sealant. It is concluded that fibrin sealant is not embryotoxic, and thus following further experimentation its eventual utilization in human in vitro fertilization/embryo transfer programs appears promising.
Similar content being viewed by others
References
Huppert LC: Induction of ovulation with clomiphene citrate. Fertil Steril 1979;31:1–8
Garcia JG, Jones GS, Wentz AC: The use of clomiphene citrate. Fertil Steril 1977;28:707–717
Jones HW Jr, Acosta AA, Garcia JE, Sandow BA, Veeck L: On the transfer of conceptuses from oocytes fertilized in vitro. Fertil Steril 1983;39:241–243
Croxatto HB, Omtiz ME, Diaz, S, Hess R, Balmaceda J, Croxatto HD: Studies of the duration of egg transport by the human oviduct. II. Ovum location at various intervals following luteinizing hormone peak. Am J Obstet Gynecol 1978;132:629–634
Jansen RPS, Turner M, Johannison E, Landgren BM, Diczhfalusy E: Cyclic changes in human endometrial surface glycoproteins: A quantitative histochemical study. Fertil Steril 1985;44:85–91
Seelich T: Tissucal (Immuro, Vierra) biochemistry and methods of application. J Head Neck Pathol 1982;3:65–69
Schlag G, Redl H (eds): Fibrin Sealant in Operative Medicine, Vol 3. Gynaecology and Obstetrics-Urology. Heidelberg, Springer-Verlag, 1986
Sher G, Knutzen V, Stratton CJ, Mortakhab MM, Allenson SG, Mayville J, Rubenstein JA, Glass MJ, Bilach SM: The development of a successful non-university ambulatory in vitro fertilization/embryo transfer program. Phase I. Fertil Steril 1984;41(4):511–518
Leung PCS, Gronow MJ, Kellow GN, Lopata A, Speirs AL, McBain JC, du Plessis YP, Johnston I: Serum supplement in human in vitro fertilization and embryo development. Fertil Steril 1984;41(1):36–39
Gates AH: Maximizing yield and developmental uniformity of eggs.In Methods in Mammalian Embryology, JD Daniel (ed), San Francisco, WH Freeman, 1970, pp 86–116
Rönnberg L, Isotalo H, Kauppila A, Martikainen H, Vihko R: Clomiphene-induced changes in endometrial receptor kinetics on the day of ovum collection after ovarian stimulation: A study of cytosol and nuclear estrogen and progestin receptors and 17β-hydroxysteroid dehydrogenase. Ann NY Acad Sci 1985;442:408–415
Birkenfeld A, Weber-Benndorf M, Mootz N, Beier HM: Effect of clomiphene on the functional morphology of oviductal and uterine mucosa. Ann NY Acad Sci 1985;442:153–167
Howard WJ Jr: Embryo transfer. Ann NY Acad Sci 1985; 442:375–380
Orlandi C, Marabini A, Vendra C, Mancini L: The use of fibrin sealant (Tissucol/Tisseel) in gynaecological surgery.In Fibrin Sealant in Operative Medicine, Vol 3. Gynaecology and Obstetrics-Urology, G Schlag, H Redl (eds). Heidelberg, Springer-Verlag, 1986, pp 63–65
Grenz HJ: Fibrin sealing in premature rupture of the membranes.In Fibrin Sealant in Operative Medicine, Vol 3. Gynaecology and Obstetrics-Urology, G Schlag, H Redl (eds). Heidelberg, Springer-Verlag, 1986 pp 98–102
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Rodrigues, F.A., Van Rensburg, J.H.J., De Vries, J. et al. The effect of fibrin sealant on mouse embryos. J Assist Reprod Genet 5, 158–160 (1988). https://doi.org/10.1007/BF01131179
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01131179