Summary
The genomic RNA of apple chlorotic leaf spot virus was translated in a rabbit reticulocyte lysate system, yielding a large, 190 K product as well as several other polypeptides of smaller size (60, 56, 22 and 15 kDa). The 22 kDa product was immunoprecipitated by an anti-ACLSV serum and comigrated with purified ACLSV coat protein. In vitro translation of RNA transcripts prepared from cloned ACLSV cDNAs demonstrates that the coat protein is synthesised by initiation on the second in frame AUG codon of the 28 kDa open reading frame located at the 3′ end of the genome. In the in vitro translation system used, the ability of various ACLSV-derived RNAs to direct the synthesis of the coat protein appears to be the result of initiation on the internal AUG codon.
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Ausubel FM, Brent R, Kingston RE, Moore DD, Smith JA, Seidman JG, Truhl K (1987) Current Protocols in Molecular Biology. Green Publishing Associates and Wiley-Interscience, New York
Bancroft JB, Rouleau M, Johnston R, Prins L, Mackie GA (1991) The entire nucleotide sequence of foxtail mosaic virus RNA. J Gen Virol 72: 2 173–2 181
Bar Joseph M, Murant AF (1982) Closterovirus group. C.M.I./A.A.B. Description of Plant Viruses, No. 260
Beccera SP, Rose JA, Hardy M, Baroudy BM, Anderson CW (1985) Direct mapping of adeno-associated virus capsid proteins B and C: a possible ACG initiation codon. Proc Natl Acad Sci USA 82: 7 919–7 923
Brault V, Candresse T, Le Gall O, Delbos RP, Lanneau M, Dunez J (1993) Genetically engineered resistance against grapevine chrome mosaic nepovirus. Plant Mol Biol 21: 89–97
Brault V, Hibrand L, Candresse T, Le Gall O, Dunez J (1989) Nucleotide sequence and genetic organisation of Hungarian grapevine chrome mosaic nepovirus RNA2. Nucleic Acids Res 17: 7 809–7 819
Candresse T, Namba S, Martelli GP (1995) Trichovirus genus description. In: Murphy FA, Faquet CM, Bishop DHL, Ghabrial SA, Jarvis AW, Martelli GP, Mayo MA, Summers MD (eds) Virus Taxonomy. Classification and Nomenclature of Viruses. Sixth Report of the International Committee on Taxonomy of Viruses. Springer, Wien New York, pp 468–470 (Arch Virol [Suppl]10)
Curran J, Kolakofsky D (1988) Ribosomal initiation from an ACG codon in the Sendai virus P/C mRNA. EMBO J 7: 245–251
Dasso MC, Jackson RJ (1989a) Efficient initiation of mammalian mRNA translation at a CUG codon. Nucleic Acids Res 17: 6 485–6 497
Dasso MC, Jackson RJ (1989b) On the fidelity of mRNA translation in the nuclease treated rabbit reticulocyte lysate system. Nucleic Acids Res 17: 3 129–3 144
Demangeat G, Greif C, Hemmer O, Fritsch C (1990) Analysis of the in vitro cleavage products of the tomato black ring virus RNA-1 encoded 250 K polyprotein. J Gen Virol 71: 1 649–1 654
Dunez J, Delbos R (1988) Closteroviruses. In: Smith IM, Dunez J, Lelliott RA, Phillips DH, Archer SA (eds) European handbook of plant diseases. Blackwell, Oxford, pp 5–7
Dunez J, Delbos R, Bertranet P (1973) Le rôle de différents facteurs de stabilisation appliqués à la purification du virus du Chlorotic Leaf spot. Ann Phytopathol 5: 255–265
Gallie DR, Sleat DE, Watts JW, Turner PC, Wilson TMA (1987) The 5′ leader sequence of tobacco mosaic virus RNA enhances the expression of foreign gene transcripts in vivo and in vitro. Nucleic Acids Res 15: 3 257–3 273
German S, Candresse T, Le Gall O, Lanneau M, Dunez J (1992) Analysis of the dsRNAs of apple chlorotic leaf spot virus. J Gen Virol 73: 767–773
German S, Candresse T, Lanneau M, Huet JC, Pernollet JC, Dunez J (1990) Nucleotide sequence and genomic organisation of apple chlorotic leaf spot closterovirus. Virology 179: 104–112
Hawkes R, Niday E, Gordon J (1982) A dot-immunobinding assay for monoclonal and other antibodies. Anal Biochem 119: 142–147
Hazelwood D, Zaitlin M (1990) Ubiquitinated conjugates are found in preparations of several plant viruses. Virology 177: 352–356
Kozak M (1987) Possible role of flanking nucleotides in recognition of the AUG initiation codon by eukaryotic ribosomes. Nucleic Acids Res 15: 8 125–8 148
Kozak M (1990) Evaluation of the fidelity of initiation of translation in reticulocyte lysates from commercial sources. Nucleic Acids Res 18: 2 828
Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680–685
Lutcke HA, Chow KC, Mickel FS, Moss KA, Kern HF, Scheele GA (1987) Selection of AUG initiation codons differs in plants and animals. EMBO J 6: 43–48
Mackenzie DJ, Tremaine JH, Stace-Smith R (1989) Organization and interviral homologies of the 3′-terminal portion of potato virus S RNA. J Gen Virol 70: 1 053–1 063
Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press Cold Spring Harbor
Martelli GP, Candresse T, Namba S (1994) Trichovirus, a new genus of plant viruses. Arch Virol 134: 451–455
Memelink J, Van der Vlugt C, Linthorst HJM, Derks AFLM, Asjes CJ, Bol JF (1990) Homologies between the genomes of a carlavirus (lily symptomless virus) and a potexvirus (lily virus X) from lily plants. J Gen Virol 71: 917–924
Morch MD, Boyer JC, Haenni AL (1988) Overlapping open reading frames revealed by complete nucleotide sequencing of turnip yellow mosaic virus genomic RNA. Nucleic Acids Res 16: 6 157–6 173
Prats AC, De Billy G, Wang P, Darlix JL (1989) CUG initiation codon used for the synthesis of a cell surface antigen coded by the murine leukemia virus. J Mol Biol 205: 363–372
Rupasov VV, Morozov SY, Kanyuka KV, Zavriev SK (1989) Partial nucleotide sequence of potato virus M RNA shows similarities to potexviruses in gene arrangement and the encoded amino acid sequences. J Gen Virol 70: 1 861–1 869
Sato K, Yoshikawa N, Takahashi T (1993) Complete nucleotide sequence of the genome of an apple isolate of apple chlorotic leaf spot virus. J Gen Virol 74: 1 927–1 931
White KA, Mackie GA (1990) Control and expression of 3′ open reading frames in clover yellow mosaic virus. Virology 179: 576–584
Yoshikawa N, Takahashi T (1988) Properties of RNAs and proteins of apple stem grooving and apple chlorotic leaf spot viruses. J Gen Virol 69: 241–245
Yoshikawa N, Takahashi T (1989) In vitro translation of apple chlorotic leaf spot virus RNA. J Gen Virol 70: 3 051–3 054
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Candresse, T., German, S., Lanneau, M. et al. In vitro translation of apple chlorotic leaf spot virus (ACLSV) RNA. Archives of Virology 141, 2031–2043 (1996). https://doi.org/10.1007/BF01718213
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DOI: https://doi.org/10.1007/BF01718213