Abstract
In order to be considered usable as synthetic seeds, encapsulated explants sown underin vitro orex vitro conditions must be able to produce whole plantlets. Ninety percent of non-encapsulated M.26 apple rootstock single nodes produced a plantlet (i.e., a well-formed shoot with a root system) after 30 days of culturein vitro if the explants were previously given a 24-hour treatment with 24.6 µM IBA and 15 g 1−1 sucrose in darkness. In contrast, when the single nodes were encapsulated in a calcium-sodium alginate bead immediately after the same treatment only 10% of the encapsulated explants formed a plantlet. Addition of growth regulators to the artificial endosperm and culture of the single nodes for root primordia initiation for 3, 6 or 9 days in darkness before encapsulation allowed production of 58%, 60% and 66% of plantlets, respectively.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Abbreviations
- AdSO4 :
-
adenine hemisulfate
- BA:
-
benzyladenine
- GA3 :
-
gibberellic acid
- IBA:
-
indolebutyric acid
- PVP:
-
polyvinyl pyrrolidone
- Synseed:
-
synthetic seed
References
Aitken-Christie J, Kozai T & Smith MAL (1995) Glossary. In: Aitken-Christié J, Kozai T & MA Lila Smith (eds). Automation and Environmental Control in Plant Tissue Culture (pp. ix-xii). Kluwer Academic Publishers, Dordrecht, The Netherlands
Ammirato PV (1985) Patterns of development in culture. In: Henke RR, Hughes KW, Constantin MJ & Hollander A (eds). Tissue Culture in Forestry and Agriculture. Basic Life Sciences, Vol 32 (pp 9–29). Plenum Press, New York, NY, USA
Bapat VA (1993) Studies on synthetic seeds of sandalwood (Santalum album L.) and mulberry (Morns indica L.). In: Redenbaugh K (ed) Synseeds: Applications of Synthetic Seeds to Crop Improvement (pp 381–408). CRC Press, Inc., Boca Raton, Ca, USA
Bapat VA & Rao PS (1990)In vivo growth of encapsulated axillary buds of mulberry (Morus indica L.). Plant Cell, Tissue and Organ Culture 20: 67–70
Barbotin JN, Nava Saucedo JE, Bazinet C, Kersulec A, Thomasset B & Thomas D (1993) Immobilization of whole cells and somatic embryos: coating process and cell-matrix interaction. In: Redenbaugh K (ed) Synseeds: Applications of Synthetic Seeds to Crop Improvement (pp 65–103). CRC Press, Inc., Boca Raton, Ca, USA
Bhattacharya N (1988) Enzyme activities during adventitious rooting. In: Davis TD, Haissig BE, Sankhla N (eds). TR Dudley, PhD (General Editor). Adventitious Root Formation in Cuttings. Advances in Plant Sciences Series, Vol 2, Chapter 7 (pp 88–101). Dioscorides Press, Portland, Or, USA
Ganapathi TR, Suprasanna P, Bapat VA & Rao PS (1992) Propagation of banana through encapsulated shoot tips. Plant Cell Rep. 11: 571–575
Hulst AC, Hens HJH, Buitelaar RM & Tramper J (1989) Determination of the effective diffusion coefficient of oxygen in gel materials in relation to gel concentration. Biotechnol. Tech. 3:199
Jacoboni A & Standardi A (1982) La moltiplicazionein vitro del melo cv. Wellspur. Rivista dell'Ortoflorofrutt. It. 3: 217–229
Piccioni E, Gasbarro E & Standardi A (1992) Indagine preliminare sull'incapsulamento di propaguli diLilium e di M.27 “vitro derivati”. Annali Facoltà di Agraria XLVI: 357–371
Piccioni E & Standardi A (1995) Encapsulation of micropropagated buds of six woody species. Plant Cell, Tissue and Organ Culture 42: 221–226
Piccioni E, Standardi A & Tutuianu VC (1996) Storage of M.26 apple rootstock encapsulated microcuttings. Advances in Horticultural Sciences 10: 185–190
Redenbaugh K (1993) Introduction. In: Redenbaugh K (ed) Synseeds: Applications of Synthetic Seeds to Crop Improvement (pp 3–7). CRC Press, Inc., Boca Raton, Ca, USA
Welander M & Pawlicki N (1993) A model system for studying root regeneration in woody species. Acta Hortic. 336: 225–230
Zimmerman RH (1984) Apple. In: Sharp WR, Evans DA, Ammirato PV & Yamada Y (eds). Handbook of Plant Cell Culture, Vol 2 (pp 369–395). MacMillan Publishing Company, New York, NY, USA
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Piccioni, E. Plantlets from encapsulated micropropagated buds of M.26 apple rootstock. Plant Cell Tiss Organ Cult 47, 255–260 (1997). https://doi.org/10.1007/BF02318980
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02318980