Skip to main content
SpringerLink
Log in

IgA antikerationocyte surface autoantibodies from two types of intercellular IgA vesiculopustular dermatosis recognize distinct isoforms of desmocollin

  • Original Paper
  • Published:
Archives of Dermatological Research Aims and scope Submit manuscript

Abstract

We previously proposed the term intercellular IgA vesiculopustular dermatosis (IAVPD) for cases showing IgA antikeratinocyte surface autoantibodies. This condition is divided into two subtypes: intraepidermal neutrophilic IgA dermatosis (IEN), showing pustule formation in the entire epidermis, and subcorneal pustular dermatosis (SPD) showing pustule formation in the uppermost epidermis. We have previously reported that serum from certain IAVPD patients reacts with bovine desmocollin (Dsc), a desmosomal cadherin. In this study we showed that two Dsc isoforms with a slightly different molecular weight were recognized by the serum from these patients. Further analysis revealed that serum from patients with the SPD type, which stained the cell surface in the uppermost epidermis with immunofluorescence, seem to react with Dsc1. By contrast, serum from patients with the IEN type, which stained the cell surface at all levels of the epidermis with immunofluorescence, seemed to react with Dsc3. This difference of distribution between the two distinct Dsc molecules may contribute to the different clinicopathological features between the IEN and SPD type of IAVPD.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Buxton RS, Cowin P, Franke WW, Garrod DR, Green KJ, King IA, Koch PJ, Magee AI, Rees DA, Stanley JR, Steinberg MS (1993) Nomenclature of the desmosomal cadherins. J Cell Biol 121: 481–483

    Article  PubMed  CAS  Google Scholar 

  2. Koulu L, Kusumi A, Steinberg MS, Klaus-Kovtun V, Stanley JR (1984) Human autoantibodies against a desmosomal core protein in pemphigus foliaceus. J Exp Med 160: 1509–1518

    Article  PubMed  CAS  Google Scholar 

  3. Stanley JR, Koulu L, Klaus-Kovtun V, Steinberg MS (1986) A monoclonal antibody to the desmosomal glycoprotein desmoglein I binds the same polypeptide as human autoantibodies in pemphigus foliaceus. J Immunol 136: 1227–1230

    PubMed  CAS  Google Scholar 

  4. Hashimoto T, Ogawa MM, Konohana A, Nishikawa T (1990) Detection of pemphigus vulgaris and pemphigus foliaceus antigens by immunoblot analysis using different antigen sources, J Invest Dermatol 94: 327–331

    Article  PubMed  CAS  Google Scholar 

  5. Koch PJ, Walsh MJ, Schmelz M, Goldschmidt MD, Zimbelmann R, Franke WW (1990) Identification of desmoglein, a constitutive desmosomal glycoprotein, as a member of the cadherin family of cell adhesion molecules. Eur J Cell Biol 53: 1–12

    PubMed  CAS  Google Scholar 

  6. Stanley JR, Yaar M, Hawley-Nelson P, Katz SI (1982) Pemphigus antibodies identify a cell surface glycoprotein synthesized by human and mouse keratinocytes. J Clin Invest 70: 281–288

    PubMed  CAS  Google Scholar 

  7. Amagai M, Klaus-Kovtun V, Stanley JR (1991) Autoantibodies against a novel epithelial cadherin in pemphigus vulgaris, a disease of cell adhesion. Cell 67: 869–877

    Article  PubMed  CAS  Google Scholar 

  8. Ebihara T, Hashimoto T, Iwatsuki K, Takigawa M, Ando M, Ohkawara A, Nishikawa T (1991) Autoantigens for IgA anti-intercellular antibodies of intercellular IgA vesiculopustular dermatosis. J Invest Dermatol 97: 742–745

    Article  PubMed  CAS  Google Scholar 

  9. Iwatsuki K, Hashimoto T, Ebihara T, Teraki Y, Nishikawa T, Kaneko F (1993) Intercellular IgA vesiculo-pustular dermatosis and related disorders: diversity of IgA anti-intercellular autoantibodies. Eur J Dermatol 3: 7–11

    Google Scholar 

  10. Huff JC, Golitz LE, Kunke KS (1985) Intraepidermal neutrophilic IgA dermatosis. N Engl J Med 313: 1643

    Article  PubMed  CAS  Google Scholar 

  11. Teraki Y, Amagai N, Hashimoto T, Kusunoki T, Nishikawa T (1991) Intercellular IgA dermatosis of childhood. Selective deposition of monomer IgAl in the intercellular space of the epidermis. Arch Dermatol 127: 221–224

    Article  PubMed  CAS  Google Scholar 

  12. Tagami H, Iwatsuki K, Iwase Y, Yamada M (1983) Subcorneal pustular dermatosis with vesiculo-bullous eruption: demonstration of subcorneal IgA deposits and a leukocyte chemotactic factor. Br J Dermatol 109: 581–587

    Article  PubMed  CAS  Google Scholar 

  13. Hashimoto T, Inamoto N, Nakamura K, Nishikawa T (1987) Intercellular IgA dermatosis with clinical features of subcorneal pustular dermatosis. Arch Dermatol 123: 1062

    Article  PubMed  CAS  Google Scholar 

  14. Chorzelski TP, Hashimoto T, Nishikawa T, Ebihara T, Dmochowski M, Ismail M, Jablonska S (1994) Unusual acantholytic bullous dermatosis associated with neoplasia and IgG and IgA antibodies against bovine desmocollins I and II. J Am Acad Dermatol 31: 351–355

    Article  PubMed  CAS  Google Scholar 

  15. Dmochowski M, Hashimoto T, Garrod DR, Nishikawa T (1993) Desmocollins I and II are recognized by certain sera from patients with various types of pemphigus, particularly Brazilian pemphigus foliaceus. J Invest Dermatol 100: 380–384

    Article  PubMed  CAS  Google Scholar 

  16. Dmochowski M, Hashimoto T, Chidgey MAJ, Yue KKM, Wilkinson RW, Nishikawa T, Garrod DR (1995) Demonstration of antibodies to bovine desmocollin isoforms in certain pemphigus sera. Br J Dermatol 133: 519–525

    Article  PubMed  CAS  Google Scholar 

  17. Holton JL, Kenny TP, Legan PK, Collins JE, Keen JN, Sharma R, Garrod DR (1990) Desmosomal glycoproteins 2 and 3 (desmocollins) show N-terminal similarity to calcium-dependent cell-cell adhesion molecules. J Cell Sci 97: 239–246

    PubMed  CAS  Google Scholar 

  18. Collins JE, Legan PK, Kenny TP, MacGarvie J, Holton JL, Garrod DR (1991) Cloning and sequence analysis of desmosomal glycoproteins 2 and 3 (desmocollins): cadherin-like desomosomal adhesion molecules with heterogeneous cytoplasmic domains. J Cell Biol 113: 381–391

    Article  PubMed  CAS  Google Scholar 

  19. Legan PK, Yue KKM, Chidgey MAJ, Holton JL, Wilkinson RW, Garrod DR (1994) The bovine desmocollin family: a new gene and expression patterns reflecting epithelial cell proliferation and differentiation. J Cell Biol 126: 507–518

    Article  PubMed  CAS  Google Scholar 

  20. Kawamura K, Watanabe K, Suzuki T, Yamakawa T, Kamiyana T, Nakagawa H, Tsurufuji S (1994) cDNA cloning and expression of a novel human desmocollin. J Biol Chem 269: 26295–26302

    PubMed  CAS  Google Scholar 

  21. Parker AE, Wheeler GN, Arnemann J, Pidsley SC, Ataliotis P, Thomas CL, Rees DA, Magee AI, Buxton RS (1992) Desmosomal glycoproteins II and III: cadherin-like junctional molecules generated by alternative splicing. J Biol Chem 266: 10438–10445

    Google Scholar 

  22. Troyanovsky SM, Eshkind LG, Troyanovsky RB; Leube RE, Franke WW (1993) Contributions of cytoplasmic domains of desmosomal cadherins to desmosome assembly and intermediate filament anchorage. Cell 72: 561–574

    Article  PubMed  CAS  Google Scholar 

  23. Theis DG, Koch PJ, Franke WW (1993) Differential synthesis of type 1 and type 2 desmocollin mRNA in human stratified epithelia. Int J Dev Biol 37: 101–110

    PubMed  CAS  Google Scholar 

  24. King IA, Arnemann J, Spurr NK, Buxton RS (1993) Cloning of the cDNA (DSC1) coding for human type 1 desmocollin and its assignment to chromosome 18. Genomics 18: 185–194

    Article  PubMed  CAS  Google Scholar 

  25. King IA, Sullivan KH, Bennet R Jr, Buxton RS (1995) The desmocollins of human foreskin epidermis: identification and chromosomal assignment of a third gene and expression patterns of the three isoforms. J Invest Dermatol 105: 314–321

    Article  PubMed  CAS  Google Scholar 

  26. Beutner EH, Jordon RE, Chorzelski TP (1968) The immunopathology of pemphigus and bullous pemphigoid. J Invest Dermatol 51: 63–80

    Article  PubMed  CAS  Google Scholar 

  27. Smith DB, Johnson KS (1988) Single-step purification of polypeptides expressed inEscherichia coli as fusions with glutathione S-transferase. Gene 67: 31–40

    Article  PubMed  CAS  Google Scholar 

  28. Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680–685

    Article  PubMed  CAS  Google Scholar 

  29. Amagai M, Karpati S, Prussick R, Klaus-Kovtun V, Stanley JR (1992) Autoantibodies against the amino-terminal cadherin-like binding domain of pemphigus vulgaris antigen are pathogenic. J Clin Invest 90: 919–926

    Article  PubMed  CAS  Google Scholar 

  30. Amagai M, Hashimoto T, Shimizu N, Nishikawa T (1994) Absorption of pathogenic autoantibodies by the extracellular domain of pemphigus vulgaris antigen (Dsg3) produced by baculovirus. J Clin Invest 94: 59–67

    PubMed  CAS  Google Scholar 

  31. Emery DJ, Diaz LA, Fairley JA, Lopez A, Taylor AF, Giudice GJ (1995) Pemphigus foliaceus and pemphigus vulgaris autoantibodies react with the extracellular domain of desmoglein-1. J Invest Dermatol 104: 323–328

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Author notes

  1. M. Dmochowski

    Present address: Department of Dermatology, University School of Medicine, 49 Przybyszewski Street, 60-355, Poznan, Poland

Authors and Affiliations

  1. Department of Dermatology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku, 160, Tokyo, Japan

    T. Ebihara & M. Dmochowski

  2. Institute of Cytosignal Research Inc., 1-2-58, Hiromchi, Shinagawa, 140, Tokyo, Japan

    K. Kawamura, T. Suzuki & S. Tsurufuji

  3. CRC Epithelial Morphogenesis Research Group, School of Biological Sciences, University of Manchester, Stopford Building, Oxford Road, M13 9PT, Manchester, UK

    M. Dmochowski & D. R. Garrod

  4. Department of Dermatology, University School of Medicine, 49 Przybyszewski Street, 60-355, Poznan, Poland

    T. Hashimoto & T. Nishikawa

Authors
  1. T. Hashimoto
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. T. Ebihara
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. M. Dmochowski
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. K. Kawamura
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. T. Suzuki
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. S. Tsurufuji
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. D. R. Garrod
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. T. Nishikawa
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Hashimoto, T., Ebihara, T., Dmochowski, M. et al. IgA antikerationocyte surface autoantibodies from two types of intercellular IgA vesiculopustular dermatosis recognize distinct isoforms of desmocollin. Arch Dermatol Res 288, 447–452 (1996). https://doi.org/10.1007/BF02505233

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02505233

Key words

Search

Navigation