Abstract
The subtilisin-sodium dodecyl sulfate complex was shown to catalyze the coupling of peptide segments on a solid phase in organic medium. By a two-stage enzymic condensation of peptide fragments on aminosilochrom (A) containing Met-Ala-Gly as a spacer, Dnp(or Boc)-Ala-Ala-Leu-Ala-Ala-Glu(OMe)-Met-Ala-Gly-A and Z-Ala-Ala-Glu(OMe)-Ala-Ala-Leu-Met-Ala-Gly-A were obtained. It was shown that the condensation products can be split off from the support using Met residue cleavage by BrCN.
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Abbreviations
- Hse:
-
homoseryl residue
- pNA:
-
p-nitroanilide
- SDS:
-
sodium dodecyl sulfate
- SPS:
-
solid phase peptide synthesis
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Kolobanova, S.V., Filippova, I.Y., Lysogorskaya, E.N. et al. The solid phase coupling of peptide segments catalyzed by the subtilisin-sodium dodecyl sulfate complex. Russ J Bioorg Chem 26, 369–374 (2000). https://doi.org/10.1007/BF02758663
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DOI: https://doi.org/10.1007/BF02758663