Abstract
Acharan sulfate (AS) is a glycosaminoglycan (GAG) prepared from the giant African snail,Achatina fulica. In this study, some biological activities of AS were evaluated on the basis of structural similarities to heparin/heparan sulfate and the biological functions of GAGs. We demonstrated that it exhibited strong immunostimulating activities as measured by carbon clearance test in mice andin vivo phagocytosis. It also exhibited a significant hypoglycemic activity in epinephrine (EP)-induced hyperglycemia as well as antifatigue effects by weight-loaded forced swimming test. And it showed hypolipidemic activities in cholesterol-rich mixture induced hyperlipidemia in rats. The above results indicate that AS has diverse biological activities and suggest therapeutically important target molecules.
Similar content being viewed by others
References
Jeong, J. A., Toida, T., Linhardt, R., and Kim, Y. S., Localization and characterization of acharan sulfate in the body of the giant African snai.Achatina fulica.Comp. Biochem. Physiol., 130, 513–519 (2001).
Johnson, J. A., and Fusaro, R. M., The quantitative enzymic determination of animal liver glycogen.Anal. Biochem., 15, 140–149 (1966).
Kreisman, S. H., Ah Mew N, Arsenault, M., Nessim S. J., Halter J. B., Vranic, M., and Marliss E. B., Epinephrine infusion during moderate intensity exercise increases glucose production and uptake.Am. J. Physiol. Endocrinol. Metab., 278, E949–957 (2000).
Kimura, Y., Kubo, M., Tani, T., Arichi, S., Ohminami, H., and Okuda, H., Studies on Scutellariae radix. III. Effects on lipid metabolism in serum, liver and fat cells of rats.Chem. Pharm. Bull., 29, 2308–2312 (1981).
Kim, Y. S., Jo, Y. Y., Chang, I. M., Toida, T., Park Y. M., and Linhardt, R. J., A new glycosaminoglycan from the giant African snai.Achatina fulica.J. Biol. Chem., 271, 11750–11755 (1996).
Linhardt, R. J., and Toida, T., Heparin oligosaccharides: New analogues, development and applications. In Carbohydrates as drugs, In Witczak, Z. J., and Nieforth. Marcel Dekker, K. A. (Eds.). New York, pp. 277–341, (1997).
Marcil, A., Harcus D, Thomas, D.Y., and Whiteway, M.,Candida albicans killing by RAW 264.7 mouse macrophage cells: effects of Candida genotype, infection ratios, and gamma interferon treatment.Infect. Immun., 70, 6319–6329 (2002).
Mariam, A., Asmawi, M. Z., and Sadikun, Hypoglycemic activity of the aqueous extract o.Orthosiphon stamineus.Fitoterapia, 57, 465–468 (1996).
Moriura, T., Matsuda, H., and Kubo, M., Pharmacological study o.Agkistrodon blomhoffii blomhoffii BOIE. V. Anti-fatigue effect of the 50% ethanol extract in acute weight-loaded forced swimming-treated rats.Biol Pharm. Bull., 19, 62–66 (1996).
Wagner, H., Proksch, A., Riess-Maurer, I., Vollmar, A., Odenthal, S., Stuppner, H., Jurcic, K., Le Turdu, M., and Heur, Y. H., Immunostimulant action of polysaccharides (heteroglycans) from higher plants. Preliminary communication.Arzneimittel-forschung, 34, 659–661 (1984).
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Shim, J.Y., Lee, Y.S., Jung, S.H. et al. Pharmacological activities of a new glycosaminoglycan, acharan sulfate isolated from the giant african snailachatina fulica . Arch Pharm Res 25, 889–894 (2002). https://doi.org/10.1007/BF02977010
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF02977010