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Mannose-binding lectin fromCurcuma zedoaria Rosc

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Abstract

A mannose-binding lectin was isolated from rhizomes of the medicinal plantCurcuma zedoaria. We used extraction with 20 mM phosphate buffer, ammonium sulfate precipitation, ion exchange chromatography on Q-Sepharose, gel filtration chromatography on Superdex 75, and reverse-phase HPLC. The purified lectin yielded a single band on SDS-PAGE that corresponded to a molecular mass of 13 kDa. This lectin exhibited hemagglutinating activity toward rabbit erythrocytes, which could be inhibited by mannose only. The lectin was digested with trypsin and its digests were analyzed using MALDI-TOF/TOF. Partial amino acid sequences were obtained from tandem mass spectra via automatedde novo sequencing, and were then identified by MS-BLAST homology searches to enable recognition of related proteins in other species. Inferred peptide sequences exhibited similarity to a mannose-binding lectin fromEpipactis helleborine, a member of the Orchidaceae.

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Literature Cited

  • Barre A, Bourne Y, van Damme EJM, Peumans WJ, Rouge P (2001) Mannosebinding plant lectins: Different structural scaffolds for a common sugar recognition process. Biochimie 83: 645–651

    Article  PubMed  CAS  Google Scholar 

  • Bradford MM (1976) Rapid and sensitive method for quantitation of microgram quantities of protein utilizing principle of protein-dye binding. Anal Biochem 72: 248–254

    Article  PubMed  CAS  Google Scholar 

  • Cao H, Sasaki Y, Fushimi H, Komatsu K (2001) Molecular analysis of medicinally used Chinese and Japanese Curcuma based on 18S rRNA gene and trnK gene sequences. Biol Pharm Bull 24: 1389–1394

    Article  PubMed  CAS  Google Scholar 

  • Chen ZH, Kai GY, Liu XJ, Lin J, Sun XF, Tang KX (2005) cDNA cloning and characterization of a mannose-binding lectin fromZingiber officinale Roscoe (ginger) rhizomes. J Biosci 30: 213–220

    Article  PubMed  CAS  Google Scholar 

  • Choi KH, Laursen RA (2000) Amino-acid sequence and glycan structures of cysteine proteases with proline specificity from ginger rhizomeZingiber officinale. Eur J Biochem 267: 1516–1526

    Article  PubMed  CAS  Google Scholar 

  • Jang MK, Sohn DH, Ryu JH (2001) A curcuminoid and sesquiterpenes as inhibitors of macrophage TNF-alpha release fromCurcuma zedoaria. Planta Med 67: 550–552

    Article  PubMed  CAS  Google Scholar 

  • Kim Kl, Kim JW, Hong BS, Shin DH, Cho Y, Kim HK, Yang HC (2000) Antitumor, genotoxicity and anticlastogenic activities of polysaccharide fromCurcuma zedoaria. Mol Cells 10: 392–398

    PubMed  CAS  Google Scholar 

  • Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680–685

    Article  PubMed  CAS  Google Scholar 

  • Lai EYC, Chyau CC, Mau JL, Chen CC, Lai YJ, Shih CF, Lin LL (2004) Antimicrobial activity and cytotoxicity of the essential oil ofCurcuma zedoaria. Amer J Chinese Med 32: 281–290

    Article  CAS  Google Scholar 

  • Lee YM, Wee HS, Ahn IP, Lee YH, An CS (2004) Molecular characterization of a cDNA for a cysteine-rich antifungal protein fromCapsicum annuum. J Plant Biol 47: 375–382

    Article  CAS  Google Scholar 

  • Matsuda H, Ninomiya K, MorikawaT, Yoshikawa M (1998) Inhibitory effect and action mechanism of sesquiterpenes fromZedoariae rhizoma on D-galactosamine/lipopolysaccharide-induced liver injury. Bioorg Med Chem Lett 8: 339–344

    Article  PubMed  CAS  Google Scholar 

  • Shevchenko A, Sunyaev S, Loboda A, Shevchenko A, Bork P, Ens W, Standing KG (2001) Charting the proteomes of organisms with unsequenced genomes by MALDI-quadrupole time of flight mass spectrometry and BLAST homology searching. Anal Chem 73:1917–1926

    Article  PubMed  CAS  Google Scholar 

  • Sophon K, Ponpimol T, Chantragan S, Jisnuson S, Apaporn B, Amorn P, Polkit S (2007) Hemagglutinating activity of Curcuma plants. Fitoterapia 78: 29–31 Suckau D, Resemann A, Schuerenberg M, Hufnagel P, Franzen J,

    Article  Google Scholar 

  • Holle A (2003) A novel MALDI LIFT-TOF/TOF mass spectrometer for proteomics. Anal Bioanal Chem 376: 952–965

    Article  PubMed  Google Scholar 

  • Syu WJ, Shen CC, Don MJ, Ou JC, Lee GH, Sun CM (1998) Cytotoxicity of curcuminoids and some novel compounds fromCurcuma zedoaha. J Nat Prod 61: 1531–1534

    Article  PubMed  CAS  Google Scholar 

  • van Damme EJM, Peumans WJ, Barre A, Rouge P (1998a) Plant lectins: A composite of several distinct families of structurally and evolutionary related proteins with diverse biological roles.

  • van Damme EJM, Peumans WJ, Pusztai A, Bardocz S (1998b) Handbook of Plant Lectins: Properties and Biomédical Applications. John Wiley and Sons, Chichester, pp 1–25

    Google Scholar 

  • van Damme EJM, Smeets K, Torrekens S, Vanleuven F, Peumans WJ (1994) Characterization and molecular-cloning of mannose-binding lectins from the orchidaceae speciesListera-Ovata, Epipactis-Helleborine andCymbidium Hybrid. Eur J Biochem 221: 769–777

    Article  PubMed  Google Scholar 

  • Vasconcelos IM, Oliveira JTA (2004) Antinutritional properties of plant lectins. Toxicon 44: 385–403

    Article  PubMed  CAS  Google Scholar 

  • Wang HX, Ng TB, Ooi VEC (1998) Lectin activity in fruiting bodies of the edible mushroomTricholoma mongolicum. Biochem Mol Biol Inter 44: 135–141

    CAS  Google Scholar 

  • Yoon HJ, Kim KP, Rark SM, Hong CB (2005) Functional mode of NtHSP17.6: A cytosolic small heat-shock protein fromNicotiana tabacum. J Plant Biol 48: 120–127

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Research Center for Bioorganic Chemistry, Department of Chemistry, Faculty of Science, Chulalongkorn University, 10330, Bangkok, Thailand

    Ponpimol Tipthara, Polkit Sangvanich & Amorn Petsom

  2. Bruker Daltonics, Permoserstrasse 15, D-04318, Leipzig, Germany

    Marcus Macth

  3. Institute of Biotechnology and Genetic Engineering, Chulalongkorn University, Bangkok, 10330, Thailand

    Amorn Petsom

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  1. Ponpimol Tipthara
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  2. Polkit Sangvanich
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  3. Marcus Macth
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  4. Amorn Petsom
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Correspondence to Amorn Petsom.

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Tipthara, P., Sangvanich, P., Macth, M. et al. Mannose-binding lectin fromCurcuma zedoaria Rosc. J. Plant Biol. 50, 167–173 (2007). https://doi.org/10.1007/BF03030626

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  • DOI: https://doi.org/10.1007/BF03030626

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