Skip to main content
SpringerLink
Log in

Evaluation of the anti-tumor effects of paclitaxel-encapsulated pH-sensitive micelles

  • Published:
Macromolecular Research Aims and scope Submit manuscript

Abstract

We evaluated the efficacy of pH-sensitive micelles, formed by methoxy poly(ethylene glycol)-b-poly(β-amino ester) (PEG-PAE), as carriers for paclitaxel (PTX), a drug currently used to treat various cancers. PTX was successful encapsulated by a film hydration method. Micelles encapsulated more than 70% of the PTX and the size of the PTX-encapsulated micelles (PTX-PM) was less than 150 nm. In vitro experiments indicated that the micelles were unstable below pH 6.5. After encapsulation of PTX within the micelles, dynamic light scattering (DLS) studies indicated that low pH had a similar demicellization effect. An in vitro release study indicated that PTX was slowly released at pH 7.4 (normal body conditions) but rapidly released under weakly acidic conditions (pH 6.0). We demonstrated the safety of micelles from in vitro cytotoxicity tests on HeLa cells and the in vivo anti-tumor activity of PTX-PM in B16F10 tumor-bearing mice. We concluded that these pH-sensitive micelles have potential as carriers for anti-cancer drugs.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. G. S. Kwon and K. Kataoka,Adv. Drug. Deliver. Rev.,16, 295 (1995).

    Article  CAS  Google Scholar 

  2. K. Kataoka, A. Harada, and Y. Nagasaki,Adv. Drug. Deliver. Rev.,47, 113 (2001).

    Article  CAS  Google Scholar 

  3. Y. P. Jung, Y. K. Son, and J. H. Kim,Macromol. Res.,15, 82 (2007).

    CAS  Google Scholar 

  4. W. J. Kim and S. W. Kim,Macromol. Res.,15, 100 (2007).

    CAS  Google Scholar 

  5. S. Y. Kim, S. H. Cho, and Y. M. Lee,Macromol. Res.,15, 646 (2007).

    CAS  Google Scholar 

  6. M. C. Jones and J. C. Eur.J. Pharm. Biopharm.,48, 101 (1999).

    Article  CAS  Google Scholar 

  7. G. S. Kwon and T. Okano,Adv. Drug. Deliver. Rev.,21, 107 (1996).

    Article  CAS  Google Scholar 

  8. R. Duncan,Pharm. Sci. Technol. Today,2, 441 (1999).

    Article  CAS  Google Scholar 

  9. A. K. Singla, A. Garg, and D. Aggarwal,Int. J. Pharm.,235, 179 (2002).

    Article  CAS  Google Scholar 

  10. J. A. Zhang, G. Anyarambhatla, L. Ma, S. Ugwu, T. Xuan, T. Sardone, and I. Ahmad,Eur. J. Pharm. Biopharm.,59, 177 (2005).

    Article  CAS  Google Scholar 

  11. S. C. Kim, D. W. Kim, Y. H. Shim, J. S. Bang, H. S. Oh, S. W. Kim, and M. H. Seo,J. Control. Release,72, 191 (2001).

    Article  CAS  Google Scholar 

  12. S. C. Lee, C. H. Kim, I. C. Kwon, H. Chung, and S. Y. Jeong,J. Control. Release,89, 437 (2003).

    Article  CAS  Google Scholar 

  13. T. Y. Kim, D. W. Kim, J. Y. Chung, S. G. Shin, S. C. Kim, D. S. Heo, N. K. Kim, and Y. J. Bang,Clin. Cancer Res.,10, 3708 (2004).

    Article  CAS  Google Scholar 

  14. O. Soga, C. F. van Nostrum, M. Fens, C. J. F. Rijcken, R. M. Schiffelers, G. Storm, and W. E. Hennink,J. Control. Release,103, 341 (2005).

    Article  CAS  Google Scholar 

  15. J. H. Kim, Y. S. Kim, S. W. Kim, J. H. Park, K. M. Kim, K. W. Choi, H. Chung, S. Y. Jeong, R. W. Park, I. S. Kim, and I. C. Kwon,J. Control. Release,111, 228 (2006).

    Article  CAS  Google Scholar 

  16. E. S. Lee, K. Na, and Y. H. Bae,Nano Lett.,5, 325 (2005).

    Article  CAS  Google Scholar 

  17. M. Stubbs, P. M. J. McSheehy, J. R. Griffiths, and C. L. Bashford,Mol. Med. Today,6, 15 (2000).

    Article  CAS  Google Scholar 

  18. M. S. Kim, S. J. Hwang, J. K. Han, E. K. Choi, H. J. Park, J. S. Kim, and D. S. Lee,Macromol. Rapid Commun.,27, 447 (2006).

    Article  CAS  Google Scholar 

  19. S. J. Hwang, M. S. Kim, J. K. Han, B. S. Kim, E. K. Choi, H. J. Park, J. S. Kim, and D. S. Lee,Macromol. Res.,15, 437 (2007).

    CAS  Google Scholar 

  20. J. Y. Ko, K. S. Park, Y. S. Kim, M. S. Kim, J. K. Han, K. M. Kim, R. W. Park, I. S. Kim, H. K. Song, D. S. Lee, and I. C. Kwon,J. Control. Release,123, 109 (2007).

    Article  CAS  Google Scholar 

  21. A. Lavasanifar, J. Samuel, and G. S. Kwon,J. Control. Release,77, 155 (2001).

    Article  CAS  Google Scholar 

  22. T. A Willey, E. J. Bekos, R. C. Gaver, G. F. Duncan, L. K. Tay, J. H. Beijnen, and R. H. Farmen,J. Chromatogr.,621, 231 (1993).

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Polymer Science and Engineering, Sungkyunkwan University, 440-746, Gyeonggi, Korea

    Jong Kwon Han, Min Sang Kim & Doo Sung Lee

  2. Department of Biochemistry and Advanced Medical Technology Cluster for Diagnosis and Prediction, School of Medicine, Kyungpook National University, 700-422, Daegu, Korea

    Yoo-Shin Kim & Rang-Woon Park

  3. Biomedical Research Center, Korea Institute of Science and Technology, 136-719, Seoul, Korea

    Kwangmeyung Kim & Ick Chan Kwon

Authors
  1. Jong Kwon Han
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Min Sang Kim
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Doo Sung Lee
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Yoo-Shin Kim
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Rang-Woon Park
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Kwangmeyung Kim
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Ick Chan Kwon
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Doo Sung Lee.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Han, J.K., Kim, M.S., Lee, D.S. et al. Evaluation of the anti-tumor effects of paclitaxel-encapsulated pH-sensitive micelles. Macromol. Res. 17, 99–103 (2009). https://doi.org/10.1007/BF03218661

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF03218661

Keywords

Search

Navigation