Abstract
Hairy root cultures of Beta vulgaris L grown in a bubble column reactor were permeabilised by exposure to B5 medium of pH 2.0. The roots released 39% of their total pigments on a 10 min exposure to B5 medium of pH 2.0 followed by return to standard 135 medium. The pigments released in the extracellular medium were recovered on an adsorption column containing XAD-16 resin. The permeabilised roots regrew and accumulated additional pigments. Comparison of this technique with the previously used techniques like oxygen starvation and temperature shock to permeabilise beet hairy roots suggest that pH mediated release of betalains can be an effective method of releasing betalains from root cultures.
This is a preview of subscription content, log in via an institution to check access.
References
Hamill JD, PD Parr AJ, Robins RJ & Rhodes MJC, Plant Cell Rep, 5 (1986) 111.
Taya M Mine K, Kino-Oka M & Tone S, J Ferment Bioeng, 73 (1992) 31.
Leathers RR, Davin C & Zyrd P, In Vitro Cell Dev Biol, 28 (1992) 39.
Wink M, In Advances in botanical research, Vol 25 (RA Leigh, D Sanders, Editors), Academic Press, SanDiego, London (1997) p 141.
Buitelar RN, Langenhoff AAN, Heidstra R & Tramper J, Enz Microbiol Tech, 13 (1991) 487.
Kondo O, Honda H, Taya M & Kobayashi T, Appl Microbiol Biotechnol, 32 (1989) 291.
Whitney PJ, Enz Microbial Tech, 14 (1992) 13.
Ramakrishnan D & Curtis WR, In Studies in plant sciences, Advances in plant biotechnology, Vol 4 (S Furasaki, DDY Ryu, Editors), Elsevier, Amsterdam (1994) p 281.
Ramakrishnan D, PhD Thisis, (1997), The Pennsylvania State University, University Park, PA-16802, USA.
Wilson PDG, In Bioreactors and biotransformations (GW Moody & PB Baker, Editors), Elsevier, London (1987) p 38
Curtis WR, Curr Opin in Biotechnol, 4 (1993) 205.
Brodelius PE, Appl Microbiol Biotechnol, 27 (1988) 561.
Kilby, NJ and Hunters CS, Appl Microbiol Biotechnol, 33 (1990) 448.
Kilby, NJ and Hunters CS, Appl Microbiol Biotechnol, 34 (1991) 478.
Kino-Oka M, Hongo Y, Taya M & Tone S, J Chem Eng Jpn, 25 (1992) 490.
Dilorio AA, Weather PJ & Cheetham RD, Appl Microbiol Biotechnol, 39 (1993) 174
Mukundan U, Bhide V, Singh G & Curtis WR, Appl Microbiol Biotechnol, 50 (1998) 241.
Gamborg OL, Miller RA & Ojima J, Exp Cell Res, 50 (1968) 151.
Connolly JH & Berlyn GP, Biotech Histochem, 71 (1996) 197
Nilsson T, Lantbrukshogskolans Annaler, 36 (1970) 170.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Mukundan, U., Bhagwat, V., Singh, G. et al. Integrated Recovery of Pigments Released from Red Beet Hairy Roots Exposed to Acidic Medium. J. Plant Biochem. Biotechnol. 10, 67–69 (2001). https://doi.org/10.1007/BF03263111
Revised:
Published:
Issue Date:
DOI: https://doi.org/10.1007/BF03263111