Skip to main content
SpringerLink
Log in

Comparative Assessment of ISSR and RAPD Marker Assays for Genetic Diversity Analysis in Jojoba [Simmondsia chinensis (Link) Schneider]

  • Short Communication
  • Published:
Journal of Plant Biochemistry and Biotechnology Aims and scope Submit manuscript

Abstract

A collection of male and female plants of ten Jojoba [Simmondsia chinensis (Link) Schneider] genotypes was analyzed with 50 RAPID and 55 ISSR markers to compare the efficiency and utility of these techniques for detecting genetic polymorphism. RAPID and ISSR analysis yielded 442 and 566 scorable amplified products, respectively, of which 60.7 and 69.3% were polymorphic. ISSRs revealed efficiency over RAPDs due to high EMR (effective multiplex ratio), DI (diversity index, mean PIC per primer) and MI (marker index) values. Jaccard similarity matrices among male plants, among female plants and between male and female plants of the ten jojoba genotypes varied between 0.705-0.784. Dendrograms generated by cluster analysis (UPGMA, NTSYS-pc) supported by bootstrap values using RAPID and ISSR datasets led to grouping of most of male and females genotypes in separate clusters. While pattern of clustering remained more or less same, the two dendrograms did differ with respect to the grouping of a few male and female genotypes. The value of the Mantel test shows poor correlation (r = 0.41) between ISSR and RAPID marker datasets.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Abbreviations

RAPD:

random amplified polymorphic DNA

ISSR:

inter simple sequence repeat

PIC:

polymorphic information content

UPGMA:

unweighted paired group method using arithmetic averages

NTSYS:

Numerical Taxonomy and Multivariate Analysis System

References

  1. Benzioni A & Forti M, In Oil crops of the world (G Robbelen, RK Downey A Ashr, Editors), McGraw Hill Publishing Co., New York (1989) pp 448–461.

    Google Scholar 

  2. Weiss EA, Oilseed crops, Longman, London (1983) pp 507–527.

    Google Scholar 

  3. Paterson AH, Tanksley SD & Sorrells ME, Adv Agronom, 46 (1991) 39.

    Article  CAS  Google Scholar 

  4. Powell W, Morgante M, Andre C, Hanafey M, Vogel J, Tingey S & Rafalski A, Mol. Breed, 2 (1996) 225.

    Article  CAS  Google Scholar 

  5. Awasthi AK, Nagaraja GM, Naik GV, Kanginakudru S, Thangavelu K & Nagaraju J, Genetics, 5 (2004) 2151.

    Google Scholar 

  6. Souframanien J & Gopalakrishna T, Theor App Genet, 109 (2004) 1687.

    Article  CAS  Google Scholar 

  7. Murray M & Thompson WF, Nucleic Acid Res, 6 (1980) 4321.

    Article  Google Scholar 

  8. Ghislain M, Zhang D, Fajardo D, Hanuman Z & Hijmans RH, Genet Resour Crop Evol, 46 (1999) 547.

    Article  Google Scholar 

  9. Chadha S & Gopalakrishna T, Current Science, 93 (2007) 688.

    CAS  Google Scholar 

  10. Rohlf FJ, NTSYS-pc:Numerical Taxonomy and Multivanate Analysis System. Version 2.2,Exeter Publications Setauket, New York (1998).

    Google Scholar 

  11. Yap IV & Nelson RJ, IRRI Discussion Paper Ser. No. 14. International Rice Research Institute, Los Baños (1996).

    Google Scholar 

  12. Mantel, N, Cancer Res, 27 (1967) 209.

    PubMed  CAS  Google Scholar 

  13. Parsons BJ, Newbury HJ, Jackson MT & Ford-Lloyd BV, Mot Breed, 3 (1997) 115.

    Article  CAS  Google Scholar 

Download references

Author information

Author notes

  1. Meenakshi Bhardwaj

    Present address: Directorate of Maize Research, Pusa Campus, New Delhi, 10012, India

Authors and Affiliations

  1. Department of Genetics, CCS Haryana Agricultural University, Hisar, 125 004, India

    Meenakshi Bhardwaj & Sanjogta Uppal

  2. Bioinformatics Section, CCS Haryana Agricultural University, Hisar, 125 004, India

    Sunita Jain

  3. Department of Biotechnology and Molecular Biology, CCS Haryana Agricultural University, Hisar, 125 004, India

    Pushpa Kharb & Rajinder K. Jain

  4. Department of Forestry, CCS Haryana Agricultural University, Hisar, 125 004, India

    Ravinder Dhillon

Authors
  1. Meenakshi Bhardwaj
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Sanjogta Uppal
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Sunita Jain
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Pushpa Kharb
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Ravinder Dhillon
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Rajinder K. Jain
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Meenakshi Bhardwaj.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Bhardwaj, M., Uppal, S., Jain, S. et al. Comparative Assessment of ISSR and RAPD Marker Assays for Genetic Diversity Analysis in Jojoba [Simmondsia chinensis (Link) Schneider]. J. Plant Biochem. Biotechnol. 19, 255–258 (2010). https://doi.org/10.1007/BF03263351

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF03263351

Key words

Search

Navigation