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A testing cascade for the detection of genetically modified rice by real-time PCR in food and its application for detection of an unauthorized rice line similar to KeFeng6

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An Erratum to this article was published on 07 April 2010

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References

  • Akiyama H, Sasaki N, Sakata K, Ohmori K, Toyota A, Kikuchi Y, Watanabe T, Furui S, Kitta K, Maitani T (2007) Indicated detection of two unapproved transgenic rice lines contaminating vermicelli products. J Agric Food Chem 55:5942–5947

    Article  CAS  PubMed  Google Scholar 

  • Anonymus (2006a) Event-specific method for the quantification of rice line LLRICE62 using real-time PCR. Protocol. http://gmo-crl.jrc.ec.europa.eu/summaries/LLRICE62_validated_Protocol.pdf

  • Anonymus (2006b) Grain testing method for detection of rice GM event LLRICE601 using RT-PCR protocols PGS0505 and PGS0476 developed by Bayer Crop Science. http://gmo-crl.jrc.ec.europa.eu/doc/LLRICE601%20sqRT-PCR%20-%20PGS0505-0476%20300806.pdf

  • Anonymus (2009) Inspection report DG/SANCO 2008-7834–MR-FINAL. http://ec.europa.eu/food/fvo/rep_details_en.cfm?rep_id=2145#

  • Babekova R, Funk T, Pecoraro S, Engel K-H, Baikova D, Busch U (2008) Duplex polymerase chain reaction (PCR) for the simultaneous detection of cryIA(b) and the maize ubiquitin promotor in the transgenic rice line KMD1. Biotechnol Biotechnol Equip 22:705–708

    CAS  Google Scholar 

  • Cao J, Duan X, McElroy D, Wu R (1992) Regeneration of herbicide resistant transgenic rice plants following microprojectile-mediated transformation of suspension culture cells. Plant Cell Rep 11:586–591

    Article  CAS  Google Scholar 

  • Cheng X, Sardana R, Kaplan H, Altosaar I (1998) Agrobacterium transformed rice plants expressing synthetic cryIA(b) and cryIA(c) genes are highly toxic to striped stem borer and yellow stam borer. Proc Nat Acad Sci USA 95:2767–2772

    Article  CAS  PubMed  Google Scholar 

  • Commission Decision of 3 April 2008 on emergency measures regarding the unauthorised genetically modified organism ‘Bt63’ in rice products (2008/289/EC). Offcial J EU 96:29–34

  • Datta SK (2004) Rice biotechnology: a need for developing countries. AgBioForum 7:31–35

    Google Scholar 

  • Hernandez M, Esteve T, Pla M (2005) Real-time polymerase chain reaction based assays for quantitative detection of barley, rice, sunflower, and wheat. J Agric Food Chem 53:7003–7009

    Article  CAS  PubMed  Google Scholar 

  • Huang J, Hu R, Rozelle S, Pray C (2008) Genetically modified rice, yields, and pesticides: assessing farm-level productivity effects in China. http://english.igsnrr.ac.cn/upload/200872510550EDCC-2008%20Huang_igsnrr.pdf

  • Ignacimuthu S, Arockiasamy S, Terada R (2000) Genetic transformation of rice: current status and future prospects. Curr Sci 79:186–195

    Google Scholar 

  • ISO 21570 (2005) Foodstuffs—methods of analysis for the detection of genetically modified organisms and derived products—quantitative nucleic acid based methods. International Organization for Standardization, Geneva, Switzerland

  • James C (2005) Global status of commercialized Biotech/GM crops. http://www.isaaa.org/resources/publications/briefs/34/download/isaaa-brief-34-2005.pdf

  • Mäde D, Degner C, Grohmann L (2006) Detection of genetically modified rice: a construct-specific real-time PCR method based on DNA sequences from transgenic Bt rice. Eur Food Res Technol 224:271–278

    Article  Google Scholar 

  • Official Collection of Test Methods (2008) Specific detection of a frequently used DNA sequence from genetically modified organisms (GMO) derived from the bar-gene of Streptomyces hygroscopicus in foodstuffs-screening method. German food and feed law—food analysis, article 64, L 00.00-124, Beuth, Berlin

  • Qiu J (2008) Keeping bollworm from the door. http://www.nature.com/news/2008/080918/full/news.2008.1118.html

  • Rong J, Song Z, Su J, Xia H, Lu B-R, Wang F (2005) Low frequency of transgene flow from Bt/CpTI rice to its nontransgenic counterparts planted at close spacing. New Phytol 168:559–566

    Article  CAS  PubMed  Google Scholar 

  • Rong J, Lu B-R, Song Z, Su J, Snow AA, Zhang X, Sun S, Chen R, Wang F (2007) Dramatic reduction of crop-to-crop gene flow within a short distance from transgenic rice fields. New Phytol 173:346–352

    Article  PubMed  Google Scholar 

  • Sawahel W (2005) Iranian Scientists produce country’s first GM rice. http://www.scidev.net/en/news/iranian-scientists-produce-countrys-first-gm-rice.html

  • Waiblinger H-U, Grohmann L, Mankertz J, Engelbert D, Pietsch K (2009) A practical approach to screen for authorised and unauthorised genetically modified plants. Anal Bioanal Chem. doi:10.1007/s00216-009-3173-2 (online first)

  • Wang Y, Johnston S (2007a) The status of GM rice R&D in China. Nat Biotechnol 25:717–718

    Article  CAS  PubMed  Google Scholar 

  • Wang Y, Johnston S (2007b) Review on GM rice risk assessment in China. UNU-IAS Working Paper No. 152. http://www.ias.unu.edu/resource_centre/152%20Yanqing%20Wang_1.pdf

  • Wu G, Cui H, Ye G, Xia Y, Sardana R, Cheng X, Li Y, Altosaar I, Shu Q (2002) Inheritance and expression of the cry1Ab gene in Bt (Bacillus thuringiensis) transgenic rice. Theor Appl Genet 104:727–734

    Article  CAS  PubMed  Google Scholar 

  • Wu W, Ye Q, Min H, Duan X, Jin W (2003) Bt-transgenic rice straw affects the culturable microbiota and dehydrogenase and phosphatase activities in a flooded paddy soil. Soil Biol Biochem 36:289–295

    Google Scholar 

  • Ye X, Al-Babili S, Klöti A, Zhang J, Lucca P, Beyer P, Potrykus I (2000) Engineering the provitamin A (beta-carotene) biosynthetic pathway into (carotenoid-free) rice endosperm. Science 287:303–305

    Article  CAS  PubMed  Google Scholar 

  • Zhang Q (2007) Strategies for developing green super rice. Proc Natl Acad Sci USA 104:16042–16409

    Google Scholar 

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Correspondence to Ralf Reiting.

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An erratum to this article can be found at http://dx.doi.org/10.1007/s00003-010-0591-1

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Reiting, R., Grohmann, L. & Mäde, D. A testing cascade for the detection of genetically modified rice by real-time PCR in food and its application for detection of an unauthorized rice line similar to KeFeng6. J. Verbr. Lebensm. 5, 185–188 (2010). https://doi.org/10.1007/s00003-010-0573-3

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