Abstract
The inheritance and genetic linkage analysis for seed dormancy and preharvest sprouting (PHS) resistance were carried out in an F8 recombinant inbred lines (RILs) derived from the cross between “CN19055” (white-grained, PHS-resistant) with locally adapted Australian cultivar “Annuello” (white-grained, PHS-susceptible). Seed dormancy was assessed as germination index (GI7) while assessment for preharvest sprouting resistance was based on whole head assay (sprouting index, SI) and visibly sprouted seeds (VI). Segregation analysis of the F2, F3 data from the glasshouse and the RIL population in 2004 and 2005 field data sets indicated that seed dormancy and PHS resistance in CN19055 is controlled by at least two genes. Heritabilities for GI7 and VI were high and moderate for SI. The most accurate method for assessing PHS resistance was achieved using VI and GI7 while SI exhibited large genotype by environment interaction. Two quantitative trait loci (QTLs) QPhs.dpivic.4A.1 and QPhs.dpivic.4A.2 were identified. On pooled data across four environments, the major QTL, QPhs.dpivic.4A.2, explained 45% of phenotypic variation for GI7, 43% for VI and 20% for SI, respectively. On the other hand, QPhs.dpivic.4A.1 which accounted for 31% of the phenotypic variation in GI7 in 2004 Horsham field trial, was not stable across environments. Physical mapping of two SSR markers, Xgwm937 and Xgwm894 linked to the major QTL for PHS resistance, using Chinese Spring deletions lines for chromosome 4AS and 4AL revealed that the markers were located in the deletion bins 4AL-12 and 4AL-13. The newly identified SSR markers (Xgwm937/Xgwm894) showed strong association with seed dormancy and PHS resistance in a range of wheat lines reputed to possess PHS resistance. The results suggest that Xgwm937/Xgwm894 could be used in marker-assisted selection (MAS) for incorporating preharvest sprouting resistance into elite wheat cultivars susceptible to PHS.
Similar content being viewed by others
References
Abawi Y, White G (2000) Early harvest for yield, quality and profit. Proceedings of the 2nd Australian Post harvest Technical Conference. In: Wright EJ, Banks HJ, Highley E (eds) Adelaide, 1–4 August 2000
Anderson JA, Sorrells ME, Tanksley SD (1993) RFLP analysis of genomic regions associated with resistance to preharvest sprouting in wheat. Crop Sci 33:453–459
Bailey PB, McKibbin RS, Lenton JR, Holdsworth MJ, Flintham JE, Gale MD (1999) Genetic map locations for orthologous Vp1 genes in wheat and rice. Theor Appl Genet 98:281–284
Burdon RD (1977) Genetic correlations as a concept for studying genotype-environment interaction in forest tree breeding. Silvae Genetica 26:168–175
Churchill GA, RV Doerge (1994) Empirical threshold values for quantitative trait mapping. Genetics 138:963–971
Derera NF (1982) The harmful harvest rain: Farrer Memorial oration, 1981. J Aust Inst Agric Sci 48(2):67–75
Derera NF (1989) The effects of preharvest rain. In: Derera NF (ed) Preharvest field sprouting in cereals. CRC Press Ltd., Boca Raton, pp 1–14
Doerge RW, Churchill GA (1996) Permutation tests for multiple loci affecting a quantitative character. Genetics 142:285–294
Dudley JW (1993) Molecular markers in plant improvement: manipulation of genes affecting quantitative traits. Crop Sci 33:660–668
Eagles HA, Bariana HS, Ogbonnaya FC, Rebetzke GJ, Hollamby GJ, Henry RJ, Henschke PH, Carter M (2001) Implementation of markers in Australia wheat breeding. Aust J Agric Res 52:1349–1356
Edwards RA, Ross AS, Mares DJ, Ellison FW, Tomlinson JD (1989) Enzymes from rain-damaged and laboratory-germinated wheat. I. Effects on product quality. J Cereal Sci 10:157–167
Flintham J, Adlam R, Bassoi M, Holdsworth M, Gale M (2002) Mapping genes for resistance to sprouting damage in wheat. Euphytica 126:39–45
Groos C, Gay G, Perretant MR, Gervais L, Bernard M, Dedryver F, Charmet G (2002) Study of the relationship between pre-harvest sprouting and grain color by quantitative trait loci analysis in a white x red grain bread-wheat cross. Theor Appl Genet 104:39–47
Holland JB, Cervantes-Martinez CT (2003) Estimating and interpreting heritability for plant breeding. Plant Breed Rev 22:9–112
Hucl P, Matus-Cadiz M (2002) W98616, a white seeded spring wheat with increased preharvest sprouting. Can J Plant Sci 82:129–131
Jin XL, Cai DL, Rong ZW (1997) Inheritance in synthetic hexaploid wheat “RSP” of sprouting tolerance derived from Aegilops tauschii Cosson. Euphytica 95:321–323
Kato K, Nakamura W, Tabiki T, Miura H, Sawada S (2001) Detection of loci controlling seed dormancy in group 4 chromosomes of wheat and comparative mapping with rice and barley genomes. Theor Appl Genet 102:980–985
King RW, Richards RA (1984) Water uptake in relation to pre-harvest sprouting damage in wheat: ear characteristics. Aust J Agric Res 35:327
Kottearachchi NS, Uchino N, Kato K, Miura H (2006) Increased grain dormancy in white-grained wheat by introgression of preharvest sprouting tolerance QTLs. Euphytica 152:421–428
Kulwal PL, Kumar N, Gaur A, Khurana P, Khurana JP, Tyagi AK, Balyan HS, Gupta PK (2005) Mapping of a major QTL for pre-harvest sprouting tolerance on chromosome 3A in bread wheat. Theor Appl Genet 111:1052–1059
Lawson RW, Godwin ID, Cooper M, Brennan PS (1997) Genetic analysis of preharvest sprouting tolerance in three wheat crosses. Aust J Agric Res 48:215–221
Lincoln S, Daly M, Lander E (1992) Constructing genetic maps with MAPMAKER/EXP3.0. Whitehead Institute Technical Report, 3rd edn. Whitehead Institute, Cambridge
Manly KF, Cudmore Jr RH, Meer JM (2001) Map Manager QTX, cross-platform software for genetic mapping. Mamm Genome 12:930–932
Mares DJ (1983) Preservation of dormancy in freshly harvested wheat. Aust J Agric Res 34:33–38
Mares DJ (1987) Preharvest sprouting tolerance in white-grained wheat. In: Mares DJ (eds) Proceedings of 4th International Symposium. Pre-harvest sprouting in cereals, Westview Press, Boulder, pp 64
Mares DJ (1989) Preharvest sprouting damage and sprouting tolerance: assay methods and instrumentation. In: Derera NF (eds) Preharvest field sprouting in cereals. CRC Press, Inc., pp 129–170
Mares DJ (1993) Genetic studies of sprouting tolerance in red and white wheat. In: Walker-Simmons, Reid JL (eds) Preharvest sprouting in cereals. American Association of Cereal Chemists, St. Paul, pp 21–29
Mares DJ (1996) Dormancy in white wheat: mechanism and location of genes. In: Noda K, Mares DJ (eds) Preharvest sprouting in cereals 1995. Centre for Academic Societies, Osaka, pp 179–184
Mares DJ, Mrva K (2001) Mapping quantitative trait loci associated with variation in dormancy in Australian wheat. Aust J Agric Res 52:1257–1266
Mares D, Mrva K, Cheong K, Williams K, Watson B, Storlie E, Sutherland M, Zou Y (2005), A QTL located on chromosome 4A associated with dormancy in wheat- and red-grained wheat of diverse origin. Theor Appl Genet 111:1357–1364
McMaster GJ, Derera NF (1976) Methodology and sample preparation when screening for sprouting damage in cereals. Cereal Res Comm 4:251–254
Mester DI, Ronin YI, Hu Y, Peng J, Nevo E, Korol AB (2003a) Efficient multipoint mapping: making use of dominant repulsion-phase markers. Theor Appl Genet 107:1102–1112
Mester D, Ronin Y, Minkov D, Nevo E, Korol A (2003b) Constructing large scale genetic maps using evolutionary strategy algorithm. Genetics 165:2269–2282
Mester DI, Ronin YI, Nevo E, Korol AB (2004) Fast and high precision algorithms for optimization in large-scale genomic problems. Comput Biol Chem 28:281–290
Mori M, Uchino N, Chono M, Kato K, Miura H (2005) Mapping QTLs for grain dormancy on wheat chromosome 3A and the group 4 chromosomes, and their combined effect. Theor Appl Genet 110:1315–1323
Noda K, Matsuura T, Maekawa M, Taketa S (2002) Chromosomes responsible for sensitivity of embryos to abscisic acid and dormancy in wheat. Euphytica 123:203–209
Noll JS, Dyke PL, Czarnecki E (1982) Expression of RL4137 type dormancy in F1 seeds of reciprocal crosses in common wheat. Can J Plant Sci 62:345–349
Ogbonnaya S, Seah I, Delibes A, Jahier J, Lopez-Braña I, Eastwood RF, Lagudah ES (2001) Molecular-genetic characterisation of nematode resistance from Aegilops ventricosa and its derivatives in wheat. Theor Appl Genet 102:263–269
Ogbonnaya FC, Imtiaz M, Hearnden P, Wilson J, Eastwood RF, Gatford KT, van Ginkel M (2006) Identification of novel gene for seed dormancy in wheat. In: Proceedings of the 13th Australasian Plant Breeding Conference, 18–21 April 2006, Christchurch, (ISBN: 978-0-86476-167-8)
Ogbonnaya FC, Ye G, Trethowan R, Dreccer F, Shepperd J, van Ginkel M (2007a) Yield of synthetic backcross-derived lines in rainfed environments of Australia. Euphytica 157:321–336. doi:10.1007/s10681-007-9381
Ogbonnaya FC, Imtiaz M, Depauw R (2007b) Haplotype diversity at pre-harvest sprouting QTLs in wheat. Genome 50:107–118. doi:10.1139/G06-142
Osa M, Kato K, Mori M, Shindo C, Torada A, Miura H (2003) Mapping QTLs for seed dormancy and the Vp1 homologue on chromosome 3A in wheat. Theor Appl Genet 106:1491–1496
Paterson AH, Sorrell MF, Obendorf RL (1989) Methods of evaluation for preharvest sprouting resistance in wheat breeding programs. Can J Plant Sci 69:681–689
Röder MS, Korzun V, Wendehake K, Plaschke J, Tixier MH, Leroy P, Ganal MW (1998) A microsatellite map of wheat. Genetics 149:2007–2023
Somers DJ, Isaac P, Edwards K (2004) A high density microsatellite consensus map for bread wheat (Triticum aestivum L.). Theor Appl Genet 109:1105–1114
Song QJ, Shi JR, Singh S, Flicks EW, Costa et al (2005) Development and mapping of microsatellite (SSR) markers in wheat. Theor Appl Genet 110:550–560
Tan MS, Sharp PJ, Lu MQ, Howes N (2006) Genetics of grain dormancy in a white wheat. Aust J Agric Res 57:1157–1165
Torada A, Shojiro Ikeguchi S, Koike M (2005) Mapping and validation of PCR-based markers associated with a major QTL for seed dormancy in wheat. Euphytica 143:251–255
Voorrips RE (2002) Mapchart: software for the graphical presentation of linkage maps and QTLs. J Hered 93:77–78
Walker-Simmons MK (1988) Enhancement of ABA responsiveness in wheat embryos at higher temperature. Plant Cell Environ 11:769–775
Wang S, Basten CJ, Zeng ZB (2006) Windows QTL cartographer 2.5. Department of Statistics, North Carolina State University, Raleigh (http://statgen.ncsu.edu/qtlcart/WQTLCart.htm)
Yousef GG, Juvik JA (2002) Enhancement of seedling emergence in sweet corn by marker-assisted backcrossing of beneficial QTL. Crop Sci 42:96–104
Acknowledgments
The authors would like to thank Jayne Wilson, Jacinta Bull, Rachel Ivess, David Burch, Erica Steadman and Julie Eder for their technical assistance. Dr. Evans Lagudah is gratefully acknowledged for supplying DNA of the wheat aneuploid lines. Department of Primary Industries, Victoria, Grains Research and Development Cooperation and CRC for Molecular Plant Breeding supported this research.
Author information
Authors and Affiliations
Corresponding author
Additional information
Communicated by R. Waugh.
Rights and permissions
About this article
Cite this article
Ogbonnaya, F.C., Imtiaz, M., Ye, G. et al. Genetic and QTL analyses of seed dormancy and preharvest sprouting resistance in the wheat germplasm CN10955. Theor Appl Genet 116, 891–902 (2008). https://doi.org/10.1007/s00122-008-0712-8
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00122-008-0712-8