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A new chromosome nomenclature system for oat (Avena sativa L. and A. byzantina C. Koch) based on FISH analysis of monosomic lines

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Abstract

Fluorescent in situ hybridization (FISH) with multiple probes was used to analyze mitotic and meiotic chromosome spreads of Avena sativa cv ‘Sun II’ monosomic lines, and of A. byzantina cv ‘Kanota’ monosomic lines from spontaneous haploids. The probes used were A. strigosa pAs120a (a repetitive sequence abundant in A-genome chromatin), A. murphyi pAm1 (a repetitive sequence abundant in C-genome chromatin), A. strigosa pITS (internal transcribed spacer of rDNA) and the wheat rDNA probes pTa71 (nucleolus organizer region or NOR) and pTa794 (5S). Simultaneous and sequential FISH employing pairs of these probes allowed the identification and genome assignation of all chromosomes. FISH mapping using mitotic and meiotic metaphases facilitated the genomic and chromosomal identification of the monosome in each line. Of the 17 ‘Sun II’ lines analyzed, 13 distinct monosomic lines were found, corresponding to four monosomes of the A-genome, five of the C-genome and four of the D-genome. In addition, 12 distinct monosomic lines were detected among the 20 ‘Kanota’ lines examined, corresponding to six monosomes of the A-genome, three of the C-genome and three of the D-genome. The results show that 19 chromosomes out of 21 of the complement are represented by monosomes between the two genetic backgrounds. The identity of the remaining chromosomes can be deduced either from one intergenomic translocation detected on both ‘Sun II’ and ‘Kanota’ lines, or from the single reciprocal, intergenomic translocation detected among the ‘Sun II’ lines. These results permit a new system to be proposed for numbering the 21 chromosome pairs of the hexaploid oat complement. Accordingly, the A-genome contains chromosomes 8A, 11A, 13A, 15A, 16A, 17A and 19A; the C-genome contains chromosomes 1C, 2C, 3C, 4C, 5C, 6C and 7C; and the D-genome consists of chromosomes 9D, 10D, 12D, 14D, 18D, 20D and 21D. Moreover, the FISH patterns of 16 chromosomes in ‘Sun II’ and 15 in ‘Kanota’ suggest that these chromosomes could be involved in intergenomic translocations. By comparing the identities of individually translocated chromosomes in the two hexaploid species with those of other hexaploids, we detected different types of intergenomic translocations.

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Acknowledgments

The authors thank Dr. H. W. Rines of the Department of Agronomy and Plant Genetics, University of Minnesota, St. Paul, USA, for providing the ‘Sun II’ monosomic lines derived from oat/maize hybrids and Dr. T. Morikawa of the University of Osaka Prefecture (Osaka), for providing the monosomic lines of A. byzantina C. Koch cv ‘Kanota’. The authors also thank the Spanish Ministerio de Educación y Ciencia (AGL2006-04165) and the Comunidad de Madrid-Universidad de Alcalá (CCG06-UAH/GEV-0380) for their funding of this study.

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  1. M. L. Irigoyen

    Present address: Department of Plant Molecular Genetics, National Center for Biotechnology, CSIC, Madrid, Spain

Authors and Affiliations

  1. Department of Cell Biology and Genetics, University of Alcalá, 28871, Alcalá de Henares (Madrid), Spain

    M. J. Sanz, Y. Loarce, E. Ferrer & A. Fominaya

  2. Department of Plant and Wildlife Sciences, Brigham Young University, 289 WIDB, Provo, UT, 84602, USA

    E. N. Jellen

Authors
  1. M. J. Sanz
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  2. E. N. Jellen
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  3. Y. Loarce
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  4. M. L. Irigoyen
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  5. E. Ferrer
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  6. A. Fominaya
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Corresponding author

Correspondence to A. Fominaya.

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Communicated by B. Friebe.

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Sanz, M.J., Jellen, E.N., Loarce, Y. et al. A new chromosome nomenclature system for oat (Avena sativa L. and A. byzantina C. Koch) based on FISH analysis of monosomic lines. Theor Appl Genet 121, 1541–1552 (2010). https://doi.org/10.1007/s00122-010-1409-3

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  • DOI: https://doi.org/10.1007/s00122-010-1409-3

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