Skip to main content

Advertisement

SpringerLink
Log in

Sublethal Effects of Waterborne Herbicides in Tropical Freshwater Fish

  • Published:
Bulletin of Environmental Contamination and Toxicology Aims and scope Submit manuscript

Abstract

The study evaluated the sublethal effects of the herbicides glyphosate (Roundup) and diuron (Hexaron) and the mixture of them, used extremely in agriculture, through biomarkers in fish. The glutathione S-transferase activity increased (74%) and catalase activity decreased (37%) at the higher exposure concentration of Hexaron in comparison to the control group, suggesting an activation of this metabolism route. Membrane damage was observed at the higher exposure of Roundup and in the mixture group compared to the control group, which can be related to the nuclear alterations observed in these exposed groups. The cholinesterase activity was also inhibited (37%) in mixture group compared to the control group and no gill morphology damage was found. The results suggested a potential synergic effect in some analysed parameters.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

Similar content being viewed by others

References

  • Aebi H (1984) Catalase in vitro. Method Enzymol 105:121–126

    Article  CAS  Google Scholar 

  • Akaishi FM, Silva de Assis HC, Jakobi SCG, Eiras-stofella DR, Stjean S, Courtenay SC, Lima EF, Wagener AL, Scofield AL, Oliveira Ribeiro CA (2004) Morphological and neurotoxicological findings in tropical freshwater fish (Astyanax sp.) after waterborne and acute exposure to water soluble fraction (WSF) of crude oil. Arch Environ Contam Toxicol 46:244–253

    CAS  Google Scholar 

  • Ballesteros ML, Wunderlin DA, Bistoni MA (2009) Oxidative stress responses in different organs of Jenynsia multidentata exposed to endosulfan. Ecotox Environ Saf 72:199–205

    Article  CAS  Google Scholar 

  • Bernet D, Schmidt H, Meier W, Burkhardt-Holm P, Wahli T (1999) Histopathology in fish: proposal for a protocol to assess aquatic pollution. J Fish Dis 22:25–34

    Article  Google Scholar 

  • Bradford M (1976) A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254

    Article  CAS  Google Scholar 

  • Ellman GL, Courtney D, Andres V Jr, Feathersthone RM (1961) A new and rapid colorimetric determination of acetylcholinesterase activity. Biochem Pharmacol 7:88–96

    Article  CAS  Google Scholar 

  • Filipak Neto F, Zanata SM, Silva de Assis HC, Nakao LS, Randi MAF, Oliveira Ribeiro CA (2008) Toxic effects of DDT and methyl mercury on the hepatocytes from Hoplias malabaricus. Toxicol In Vitro 22:1705–1713

    Article  CAS  Google Scholar 

  • Grisolia CK (2002) A comparison between mouse and fish micronucleus test using cyclophosphamide, mitomycin C and various pesticides. Mutat Res 518:145–150

    CAS  Google Scholar 

  • Heddle JA (1973) A rapid in vivo test for chromosomal damage. Mutat Res 18:187–190

    Article  CAS  Google Scholar 

  • Isik I, Celik I (2008) Acute effects of methyl parathion and diazinon as inducers for oxidative stress on certain biomarkers in various tissues of rainbow trout (Oncorhynchus mykiss). Pestic Biochem Physiol 92:38–42

    Article  CAS  Google Scholar 

  • Jiang ZY, Hunt JV, Wolff SP (1992) Ferrous ion oxidation in the presence of xylenol orange for detection of lipid hydroperoxide in low density lipoprotein. Anal Biochem 202:384–389

    Article  CAS  Google Scholar 

  • Keen JH, Habig WH, Jakoby WB (1976) Mechanism for several activities of the glutathione S-transferases. J Biol Chem 251:6183–6188

    CAS  Google Scholar 

  • Langiano VC, Martinez CBR (2008) Toxicity and effects of a glyphosate-based herbicide on the Neotropical fish Prochilodus lineatus. Comp Biochem Physiol 147:222–231

    Google Scholar 

  • Peixoto F (2005) Comparative effects of the Roundup and glyphosate on mitochondrial oxidative phosphorylation. Chemosphere 61:1115–1122

    Article  CAS  Google Scholar 

  • Ploch SA, Lee YP, MacLean E, Di Giulio RT (1999) Oxidative stress in liver of brown bullhead and channel catfish following exposure to tert-butyl hydroperoxide. Aquat Toxicol 46:231–240

    Article  CAS  Google Scholar 

  • Saglio P, Trijasse S (1998) Behavioural responses to atrazine and diuron in gold fish. Arch Environ Contam Toxicol 35:484–491

    Article  CAS  Google Scholar 

  • Souza MD, Boeira RC, Gomes MAF (2000) Adsorção e dessorção de diuron em solos tropicais. Pesticidas R Ecotoxicol e Meio Ambiente 10:113–124

    Google Scholar 

  • Sturm A, Silva de Assis HC, Hansen PD (1999) Cholinesterases of marine teleost fish: enzymological characterization and potential use in the monitoring of neurotoxic contamination. Mar Environ Res 47:389–398

    Article  CAS  Google Scholar 

  • Teisseire H, Vernet G (2000) Is the “Diuron Effect” due to a herbicide strengthening of antioxidative defenses of Lemna minor? Comp Biochem Physiol 66:153–160

    CAS  Google Scholar 

  • Wang X, Wang C, Tan C (2005) Degradation and metabolism of hexazinone by two isolated bacterial strains from soil. Chemosphere 61:1468–1474

    Article  CAS  Google Scholar 

  • World Health Organization (WHO) (1994) Glyphosate, international programme on chemical safety. Environ Health Criteria 159, Geneva, Switzerland, pp 110–112

  • Yi X, Ding H, Lu Y, Liu H, Zhang M, Jiang W (2007) Effects of long-term alachlor exposure on hepatic antioxidant defense and detoxifying enzyme activities in crucian carp (Carassius auratus). Chemosphere 68:1576–1581

    Article  CAS  Google Scholar 

  • Zhonglin Z, Zhengjun S, Daoji C (1998) Evaluation on effect of hexazinone on eco-environment. Adv Environ Sci 6:11–20

    Google Scholar 

Download references

Acknowledgments

This research was supported in part by CNPq (Brazilian Agency for Science and Technology).

Author information

Authors and Affiliations

  1. Department of Pharmacology, Federal University of Paraná, PO Box 19031, Curitiba, PR, 81.531-990, Brazil

    Stéfani Cibele Rossi, Manuela Dreyer da Silva & Helena Cristina Silva de Assis

  2. Department of Genetic, Federal University of Paraná, PO Box 19031, Curitiba, PR, 81.531-990, Brazil

    Laercio Dante Stein Piancini & Marta Margarete Cestari

  3. Department of Cellular Biology, Federal University of Paraná, PO Box 19031, Curitiba, PR, 81.531-990, Brazil

    Ciro Alberto Oliveira Ribeiro

Authors
  1. Stéfani Cibele Rossi
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Manuela Dreyer da Silva
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Laercio Dante Stein Piancini
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Ciro Alberto Oliveira Ribeiro
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Marta Margarete Cestari
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Helena Cristina Silva de Assis
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Helena Cristina Silva de Assis.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Rossi, S.C., Dreyer da Silva, M., Piancini, L.D.S. et al. Sublethal Effects of Waterborne Herbicides in Tropical Freshwater Fish. Bull Environ Contam Toxicol 87, 603–607 (2011). https://doi.org/10.1007/s00128-011-0397-6

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00128-011-0397-6

Keywords

Search

Navigation