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Bioaugmentation-Assisted Phytostabilisation of Abandoned Mine Sites in South West Sardinia

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Abstract

Bioaugmentation-assisted phytoremediation implies the administration of selected plant growth promoting bacteria, which significantly improve plant growth and sequestration of heavy metals. In this work, 184 bacterial strains associated with roots of Pistacia lentiscus were isolated from plants spontaneously growing in the abandoned Sardinian mining areas (SW Sardinia, Italy) and phylogenetically characterised. Twenty-one bacterial isolates were assayed for properties relevant for plant growth promotion and metal tolerance. Five different strains, belonging to the genera Novosphingobium, Variovorax, Streptomyces, Amycolatopsis, Pseudomonas, were selected based on their properties for the greenhouse phytoremediation tests. Among the tested inocula, the strain Variovorax sp. RA128A, able to produce ACC deaminase and siderophore, was able to significantly enhance germination and increase length and weight of shoots and roots. Irrespective of the applied treatment, mastic shrub was able to accumulate Cd, Pb and Zn especially in roots.

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Acknowledgments

This research has been funded by the Regional Sardinian Government, in the framework of “L.R. 7/2007, Promotion of scientific research and technological innovation in Sardinia”.

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Authors and Affiliations

  1. Department of Biomedical Sciences, University of Cagliari, Monserrato, Cagliari, Italy

    E. Tamburini, S. Sergi & L. Serreli

  2. Department of Life and Environmental Sciences, University of Cagliari, Cagliari, Italy

    L. Serreli & G. Bacchetta

  3. Institute of Environmental Geology and Geoengineering, National Research Council, Cagliari, Italy

    S. Milia, G. Cappai & A. Carucci

  4. Department of Civil, Environmental Engineering and Architecture, University of Cagliari, Cagliari, Italy

    G. Cappai & A. Carucci

Authors
  1. E. Tamburini
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  2. S. Sergi
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  3. L. Serreli
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  4. G. Bacchetta
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  5. S. Milia
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  6. G. Cappai
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  7. A. Carucci
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Correspondence to E. Tamburini.

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Tamburini, E., Sergi, S., Serreli, L. et al. Bioaugmentation-Assisted Phytostabilisation of Abandoned Mine Sites in South West Sardinia. Bull Environ Contam Toxicol 98, 310–316 (2017). https://doi.org/10.1007/s00128-016-1866-8

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  • DOI: https://doi.org/10.1007/s00128-016-1866-8

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