Abstract
Production of red flesh dragon fruit (Hylocereus polyrhizus) was hampered by Colletotrichum sp. Pre-harvest application of azoxystrobin and difenoconazole mixture is recommended, therefore, a selective and sensitive multi residues analytical method is required in monitoring and evaluating the commodity’s safety. LC–MS/MS is a well-established analytical technique for qualitative and quantitative determination in complex matrices. However, this method is hurdled by co-eluted coextractives interferences. This work evaluated the pH effect of acetate buffered and citrate buffered QuEChERS sample preparation in their effectiveness of matrix effect reduction. Citrate buffered QuEChERS proved to produce clean final extract with relative matrix effect 0.4%–0.7%. Method validation of the selected sample preparation followed by LC–MS/MS for whole dragon fruit, flesh and peel matrices fortified at 0.005, 0.01, 0.1 and 1 g/g showed recoveries 75%–119%, intermediate repeatability 2%–14%. The expanded uncertainties were 7%–48%. Based on the international acceptance criteria, this method is valid.
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References
Anastassiades M, Scherbaum E, Tasdelen B, Stajnbaher D, (2007) Recent developments in QuEChERS methodology for pesticide multiresidue analysis, In: Ohkawa H, Miyagawa H, Lee PW (eds), Pesticide chemistry. Crop protection, public health, environmental safety. Wiley, Weinheim, pp 436–458. https://s3.amazonaws.com/academia.edu.documents/44644339/Pesticide_Chemistry_-_Crop_Protection__Public_Health__Environmental_Safety_Wiley-VCH__2007.pdf. Accessed 06 June 2017
AOAC Official Method (2007).01, Pesticide residues in foods by acetonitrile extraction and partitioning with magnesium sulfate gas chromatography/mass spectrometry and liquid chromatography/tandem mass spectrometry. https://nucleus.iaea.org/fcris/methods/MRM_AOAC_2007_01.pdf. Accessed 16 Nov 2011
Chawla S, Patel HK, Gor HN, Vaghela KM, Solank PP, Shah PG (2017) Evaluation of matrix effects in multiresidue analysis of pesticide residues in vegetables and spices by LC–MS/MS. J AOAC Int 100(3): 616–623 https://doi.org/10.5740/jaoacint.17-0048. Accessed 08 July 2017
Codex Alimentarius Commission Guideline CAC/GL 40 1993 (1993), Rev.1-2003 Guidelines on good laboratory practice in residue analysis, http://www.fao.org/fao-who-codexalimentarius/standards/list-of-standards/en/. Accessed 15 Oct 2015
Codex Alimentarius Commission Guideline CAC/GL 59-2006 (2006) (Amendment 1-2011), Guidelines on estimation of uncertainty of results http://www.fao.org/fao-who-codexalimentarius/standards/list-of-standards/en/. Accessed 05 July 2016
CX/PR 16/48/13 (2016) Proposed draft guidelines on performance criteria for methods of analysis for the determination of pesticide residues, step 5, Joint FAO/WHO Food Standards Programme, Codex Committee On Pesticide Residues, 48th Session Chongqing, P.R. China, 25–30 April 2016, http://www.fao.org/fao-who-codexalimentarius/meetings-reports/en. Accessed 12 Jun 2016
Dembitsky VM, Poovarodom S, Leontowicz H, Leontowicz M, Vearasilp S, Trakhtenberg S, Gorinstein S (2011) The multiple nutrition properties of some exotic fruits: biological activity and active metabolites. Food Res Int 44:(2011) 1671–1701. https://doi.org/10.1016/j.foodres.2011.03.003. Accessed 09 Aug 2013
Diningsih E, Yusuf ES, Rahardjo IB, Sulyo Y (2012) Pengujian Lapangan Efikasi Amistar Top 325 Sc (Azoxistrobin 200 g/L + Difenokonazol 125 g/L) Terhadap Penyakit Antraknosa (Colletotrichum gloeosporioides (Penz) Penz. & Sacc) Pada Tanaman Buah Naga (Hylocereus undatus (Haworth) Britton & Rose) (Izin Perluasan enggunaan dari PT Syngenta Indonesia. Jakata), Perhimpunan Fitopatologi Indonesia (PFI) Komda Segunung
EN 15662:2008 (2008) Foods of plant origin: determination of pesticide residues using GC–MS and/or LC–MS/MS following acetonitrile extraction/partitioning and clean-up by dispersive SPE–QuEChERS-method, European Committee For Standardization, Brussels http://www.chromnet.net/Taiwan/QuEChERS_Dispersive_SPE/QuEChERS_%E6%AD%90%E7%9B%9F%E6%96%B9%E6%B3%95_EN156622008_E.pdf. Accessed 06 Aug 2013
European Commission Directorate General For Health And Food Safety (2015) Guidance document on analytical quality control and method validation procedures for pesticide residues and analysis in food and feed, SANTE/11945/2015
FAO Manual (2016) FAO manual on the submission and evaluation of pesticide residues data for the estimation of maximum residues levels in food and feed, 3rd edn. Food and Agriculture of the United Nations, Rome http://www.fao.org/fileadmin/templates/agphome/documents/Pests_Pesticides/JMPR/Manual/FAO_manual_3rd_edition_Final.pdf. Accessed 05 Nov 2016
Jamilah B, Shu CE, Kharidah M, Dzulkifly MA, Noranizan A, (2011) Physico-chemical characteristics of red pitaya (Hylocereus polyrhizus) Peel., Int Food Res J 18:279–286 http://psasir.upm.edu.my/16208/1/16208.pdf. Accessed 09 Aug 2013
King R, Bonfiglio R, Fernandez-Metzler C, Miller-Stein C, Olah T (2000) mechanistic investigation of ionization suppression in electrospray ionization. J Am Soc Mass Spectrom 11:942–950 https://ac.els-cdn.com/S104403050000163X/1-s2.0-S104403050000163X-main.pdf. Accessed 15 Feb 2017
Lehotay SJ, Son KA, Kwon H, Koesukwiwat U, Fu W, Mastovska K, Hoh E, Leepipatpiboon N, (2010) Comparison of QuEChERS sample preparation methods for the analysis of pesticide residues in fruits and vegetables. J Chromatogr A 1217:2548–2560. https://www.sciencedirect.com/science/article/pii/S0021967310000816. Accessed 06 Jun 2017
Pesticide Data Program (2016) Data and Instrumentation SOP No.: PDP-DATA Revision: 4. United States Department of Agriculture Agricultural Marketing Service, Science & Technology. https://www.ams.usda.gov/sites/default/files/media/PDP%20DATA%20SOP.pdf. Accessed 11 Jan 2017
Rebecca OPS, Boyce AN, Chandran S (2010) Pigment identification and antioxidant properties of red dragon fruit (Hylocereus polyrhizus). Afr J Biotech 9(10), 1450–1454, http://www.academicjournals.org/AJB. Accessed 06 July 2016
Sumardiyono C, Widiastuti A (2012) Laporan Penyakit-Penyakit Pada Buah Naga, Laboratorium Toksikologi Pestisida. Jurusan Hama dan Penyakit Tumbuhan, Fakultas Pertanian UGM, Yogyakarta
Acknowledgements
High appreciation is extended to Dr. Wayne Jiang and Prof. Dr. Arpad Ambrus for their assistance in this GLP analytical study. This study is part of the STDF/PG/337 Magnitude of Azoxystrobin and Difenoconazole Residues in Dragon fruit, a project funded by Standards and Trade Development Facility (STDF), World Trade Organization.
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Noegrohati, S., Hernadi, E. & Asviastuti, S. Matrix Effect Evaluation and Method Validation of Azoxystrobin and Difenoconazole Residues in Red Flesh Dragon Fruit (Hylocereus polyrhizus) Matrices Using QuEChERS Sample Preparation Methods Followed by LC–MS/MS Determination. Bull Environ Contam Toxicol 100, 821–826 (2018). https://doi.org/10.1007/s00128-018-2317-5
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DOI: https://doi.org/10.1007/s00128-018-2317-5