Skip to main content

Advertisement

SpringerLink
Log in

EDTA-Assisted Metal Uptake in Raphanus sativus L. and Brassica oleracea L.: Assessment of Toxicity and Food Safety

  • Published:
Bulletin of Environmental Contamination and Toxicology Aims and scope Submit manuscript

Abstract

A study was conducted to determine the effect of ethylenediaminetetraacetic acid (EDTA) on phytoextraction potential of radish and cabbage. Plant biomass, photosynthetic pigments, proline and phenolics were significantly affected by the accumulation of heavy metals (HM). The metal uptake potential was increased significantly by the application of EDTA. Target hazard quotient (THQ) associated with exposure of these contaminants to food chain was calculated. Agronomic interventions to increase mineral levels in crops often increases the leaf concentrations only, the mineral concentration in edible portions are not increased at desired level due to low mobility of Zn in phloem. Since the leaves of both these crops are edible and a component of staple vegetarian diet, biofortification through Zn present in soil and its solubilization and mobilization through chelators can be implemented. However in no such instance these crops should be consumed when grown on Pb contaminated soil due to associated hazards.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

Similar content being viewed by others

References

  • Bates L, Waldren RP, Teare ID (1973) Rapid determination of free proline for water-stress studies. Plant Soil 39:205–207

    Article  CAS  Google Scholar 

  • Bray HG, Thorpe WV (1954) Analysis of phenolic compounds of interest in metabolism. Methods Biochem Anal 1:27–52

    CAS  Google Scholar 

  • Epstein AL, Gussman CD, Blaylock MJ, Yermiyahu U, Huang JW, Kapulnik Y, Orser CS (1999) EDTA and Pb–EDTA accumulation in Brassica juncea grown in Pb-amended soil. Plant Soil 208:87–94

    Article  CAS  Google Scholar 

  • Evangelou MHW, Nauer U, Ebel M, Schaeffer A (2007) The influence of EDDS and EDTA on the uptake of heavy metals of Cd and Cu from soil with tobacco Nicotianna tabacum. Chemosphere 68:345–353

    Article  CAS  Google Scholar 

  • Garba ST, Maina HM, Oseamehon SA, Barminas JT (2012) EDTA-assisted phytoremediation of heavy metal contaminated soil by Eleusine indica L. Gearth. J Environ Chem Ecotoxicol 4(5):103–109

    CAS  Google Scholar 

  • Guala SD, Veg FA, Covelo EF (2010) The dynamics of heavy metals in plant-soil interactions. Ecol Modell 221:1148–1152

    Article  CAS  Google Scholar 

  • Hayat S, Hayat Q, Alyemeni MN, Wani AS, Pichtel J, Ahmad A (2012) Role of proline under changing environments A review. Plant Signal Behav 7(11):1456–1466

    Article  CAS  Google Scholar 

  • Huang G-Y, Wang Y-S (2010) Physiological and biochemical responses in the leaves of two mangrove plant seedlings (Kandelia candel and Bruguiera gymnorrhiza) exposed to multiple heavy metals. J Hazard Mater 182:848–854

    Article  CAS  Google Scholar 

  • Krämer U (2010) Metal hyperaccumulation in plants. Annu Rev Plant Biol 61:517–534

    Article  CAS  Google Scholar 

  • Kuzminov FI, Brown CM, Fadeev VV, Gorbunov MY (2013) Effects of metal toxicity on photosynthetic processes in coral symbionts, Symbiodinium spp. J Exp Mar Biol Ecol 446:216–227

    Article  CAS  Google Scholar 

  • Lichtenthaler HK (1987) Chlorophylls and carotenoids: pigments of photosynthetic biomembranes. Methods Enzymol 148:350–382

    Article  CAS  Google Scholar 

  • Lim JM, Salido AL, Butcher DJ (2004) Phytoremediation of lead using Indian mustard (Brassica juncea) with EDTA and electrodics. Microchem J 76:3–9

    Article  CAS  Google Scholar 

  • Luo C, Liu C, Wang Y, Liu X, Li F, Zhang G, Li X (2011) Heavy metal contamination in soils and vegetables near an e-waste processing site, south China. J Hazard Mater 186:481–490

    Article  CAS  Google Scholar 

  • National Institute of Nutrition (2011) Dietary guidelines for Indians: a manual. NIN, Hyderabad

    Google Scholar 

  • Neilson S, Rajakaruna N (2012) Roles of rhizospheric processes and plant physiology in applied phytoremediation of contaminated soils using brassica oilseeds. In: Anjum NA, Ahmad I, Pereira ME, Duarte AC, Umar S, Khan NA (Eds) The plant family Brassicaceae. Springer, Dordrecht, Vol 21, pp 313–330

    Chapter  Google Scholar 

  • Prasad M, de Oliveira Freitas H (2003) Metal hyperaccumulation in plants—biodiversity prospecting for phytoremediation technology. Electron J Biotechnol 6(3):285–321

    Article  Google Scholar 

  • Schmidt R, Bancroft I (2010) Genetics and genomics of the Brassicaceae. Springer, Berlin

    Google Scholar 

  • Shanmugam V, Lo JC, Wu CL, Wang SL, Lai CC, Connolly EL (2011) Differential expression and regulation of iron-regulated metal transporters in Arabidopsis halleri and Arabidopsis thaliana- the role in zinc tolerance. New Phytol 190(1):125–137

    Article  CAS  Google Scholar 

  • Sun Y, Zhou Q, An J, Liu W, Liu R (2009) Chelator-enhanced phytoextraction of heavy metals from contaminated soil irrigated by industrial wastewater with the hyperaccumulator plant (Sedum alfredii Hance). Geoderma 150:106–112

    Article  CAS  Google Scholar 

  • Szczygłowska M, Piekarska A, Konieczka P, Namiesnik J (2011) Use of Brassica plants in the phytoremediation and biofumigation processes. Int J Mol Sci 12:7760–7771

    Article  CAS  Google Scholar 

  • USEPA (2003) Integrated risk information system database (IRIS). USEPA, Washington

  • USEPA (2011) Exposure factors handbook 2011 edition (Final). USEPA, Washington

    Google Scholar 

  • Venkatachalam P, Jayalakshmi N, Geetha N, Sahi SV, Sharma NC, Rene ER, Sarkar SK, Favas PJC (2017) Accumulation efficiency, genotoxicity and antioxidant defense mechanisms in medicinal plant Acalypha indica L. under lead stress. Chemosphere 171:544–553

    Article  CAS  Google Scholar 

  • White PJ, Broadley MR (2011) Physiological limits to zinc biofortification of edible crops. Frontiers Plant Sci. 2(80):1–11

    Google Scholar 

  • Zaier H, Ghnaya T, Ben Rejeb K, Lakhdar A, Rejeb S, Jemal F (2010) Effects of EDTA on phytoextraction of heavy metals (Zn, Mn and Pb) from sludge-amended soil with Brassica napus. Bioresource Technol 101:3978–3983

    Article  CAS  Google Scholar 

  • Zhou H, Yang WT, Zhou X, Liu L, Gu JF, Wang WL, Zou JL, Tian T, Peng PQ, Liao BH (2016) Accumulation of heavy metals in vegetable species planted in contaminated soils and the health risk assessment. Int J Environ Res Public Health 13:289–300

    Article  CAS  Google Scholar 

Download references

Acknowledgements

Junior Research Fellowship to Ritu Chaturvedi (08/109(0011)/2013-EMR-I) by the Council of Scientific and Industrial Research is duly acknowledged. The support of Fundação para a Ciência e a Tecnologia, through the strategic Project UID/MAR/04292/2019 granted to MARE is gratefully acknowledged. We also thank University Grants Commission for granting Post Doctoral Fellowship (/PDFSS-2014-15-SC-UTT-8854) to Mayank Varun. We wish to acknowledge Dr. B.R. Ambedkar University, Agra for providing facilities.

Author information

Authors and Affiliations

  1. Department of Botany, St. John’S College, Agra, UP, 282 002, India

    Ritu Chaturvedi, Mayank Varun & Manoj S. Paul

  2. School of Life Sciences and the Environment, University of Trás-Os-Montes E Alto Douro, 5001-801, Vila Real, Portugal

    Paulo Favas

  3. MARE – Marine and Environmental Sciences Centre, Faculty of Sciences and Technology, University of Coimbra, 3000-517, Coimbra, Portugal

    Paulo Favas & João Pratas

  4. Department of Earth Sciences, Faculty of Sciences and Technology, University of Coimbra, 3001-401, Coimbra, Portugal

    João Pratas

  5. Instituto Do Petróleo E Geologia (Institute of Petroleum and Geology), Rua Delta 1, Aimutin Comoro, Dili, Timor-Leste

    João Pratas

Authors
  1. Ritu Chaturvedi
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Paulo Favas
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. João Pratas
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Mayank Varun
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Manoj S. Paul
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Ritu Chaturvedi.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Chaturvedi, R., Favas, P., Pratas, J. et al. EDTA-Assisted Metal Uptake in Raphanus sativus L. and Brassica oleracea L.: Assessment of Toxicity and Food Safety. Bull Environ Contam Toxicol 103, 490–495 (2019). https://doi.org/10.1007/s00128-019-02651-9

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00128-019-02651-9

Keywords

Search

Navigation