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Early osteoarthritis and microdialysis: a novel in vivo approach for measurements of biochemical markers in the perisynovium and intraarticularly

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Knee Surgery, Sports Traumatology, Arthroscopy Aims and scope

Abstract

The microdialysis technique was evaluated as a possible method to obtain local measurements of biochemical markers from knee joints with degenerative changes. Seven patients scheduled for arthroscopy of the knee due to minor to moderate degenerative changes had microdialysis catheters inserted under ultrasonographic guidance, intraarticularly and in the synovium-close tissue. Catheters were perfused at a rate of 2 μl/min for approximately 100 min with a Ringer solution containing radioactively labeled glucose, and the positions of the catheters were later visualized during arthroscopy. All intraarticular catheters and 6/7 subsynovial catheters were positioned correctly. Relative recovery (RR) was intraarticularly 0.64 ± 0.02 (mean ± SEM) and synovium-close 0.54 ± 0.06. Mean values of cartilage oligomeric matrix protein (COMP), aggrecan and glucosyl–galactosyl–pyridinoline in the intraarticular dialysates were 18.1 ± 7.0 U/l, 243.6 ± 108.6 ng/ml and 108.0 ± 29.0 pmol/ml, respectively. COMP and glucosyl–galactosyl–pyridinoline concentrations were significantly higher intraarticularly compared to perisynovial tissue (P < 0.05), whereas for aggrecan, no significant difference was found (P = 0.06). The microdialysis method can be used for intraarticular and subsynovial determination of metabolites in human knees at these sites. The present methodology displays a potential for future studies of simultaneous biochemical changes within and around joints.

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Acknowledgments

Danish Rheumatism Association, Danish Ministry of Health, Internal Affairs, Danish National Research Council, Danish Medical Research Council.

Conflict of interest statement

No authors of this manuscript have conflicts of interest that have influenced the work presented in this article.

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Authors and Affiliations

  1. Institute of Sports Medicine, Department of Orthopedic Surgery, Center for Healthy Aging, Bispebjerg Hospital, Bld. 8, 2nd floor, Bispebjerg Bakke 23, 2400, Copenhagen NV, Denmark

    Ida Carøe Helmark, U. R. Mikkelsen, M. R. Krogsgaard, M. C. H. Petersen, H. Langberg & M. Kjaer

  2. Faculty of Health Sciences, University of Copenhagen, Copenhagen, Denmark

    Ida Carøe Helmark, U. R. Mikkelsen, M. C. H. Petersen, H. Langberg & M. Kjaer

  3. Department of Anaesthesiology, Bispebjerg Hospital, Copenhagen, Denmark

    B. Belhage

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  1. Ida Carøe Helmark
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  2. U. R. Mikkelsen
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  3. M. R. Krogsgaard
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  4. B. Belhage
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  5. M. C. H. Petersen
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  6. H. Langberg
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  7. M. Kjaer
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Correspondence to Ida Carøe Helmark.

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Helmark, I.C., Mikkelsen, U.R., Krogsgaard, M.R. et al. Early osteoarthritis and microdialysis: a novel in vivo approach for measurements of biochemical markers in the perisynovium and intraarticularly. Knee Surg Sports Traumatol Arthrosc 18, 1617–1623 (2010). https://doi.org/10.1007/s00167-010-1115-3

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  • DOI: https://doi.org/10.1007/s00167-010-1115-3

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