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Rheinheimera riviphila sp. nov., isolated from a freshwater stream

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Abstract

Strain KYPC3T, isolated from a freshwater stream in Taiwan, was characterized using a polyphasic taxonomy approach. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain KYPC3T belonged to the genus Rheinheimera. Strain KYPC3T exhibited 16S rRNA gene sequence similarity values of 94.8–97.9% to the type strains of species of the genus Rheinheimera. Strain KYPC3T was most closely related to Rheinheimera chironomi K19414T with 16S rRNA gene sequence similarity of 97.9%. Cells of strain KYPC3T were Gram-stain negative, aerobic, motile by means of a single-polar flagellum, non-spore forming, coccoid or short rods surrounded by a thick capsule and forming off-white coloured colonies. Growth occurred at 15–30 °C (optimum, 20–25 °C), at pH 6–8 (optimum, pH 7) and with 0–0.5% NaCl (optimum, 0%). The major fatty acids (> 10%) of strain KYPC3T were C12:0 3-OH, summed feature 3 (C16:1ω7c and/or C16:1ω6c) and C16:0. The polar lipid profile consisted of phosphatidylethanolamine, phosphatidylglycerol, an uncharacterized aminophospholipid, an uncharacterized phospholipid and an uncharacterized lipid. The major isoprenoid quinone was Q-8. The draft genome was approximately 4.75 Mb in size with a G + C content of 49.8 mol%. The DNA–DNA relatedness of strain KYPC3T with respect to recognized species of the genus Rheinheimera was significantly less than 70%. On the basis of phenotypic and genotypic properties and phylogenetic inference, strain KYPC3T should be classified as a novel species of the genus Rheinheimera, for which the name Rheinheimera riviphila sp. nov. is presented. The type strain is KYPC3T (= BCRC 81008T = LMG 29729T = KCTC 52440T).

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Authors and Affiliations

  1. Laboratory of Microbiology, Department of Seafood Science, National Kaohsiung University of Science and Technology, No. 142, Hai-Chuan Rd., Nan-Tzu, Kaohsiung, 811, Taiwan

    Wen-Ming Chen & Wei-Ting Chen

  2. College of Agriculture and Natural Resources, Department of Soil and Environmental Sciences, National Chung Hsing University, Taichung, 402, Taiwan

    Chiu-Chung Young

  3. Department of Marine Biotechnology, National Kaohsiung University of Science and Technology, No. 142, Hai-Chuan Rd., Nan-Tzu, Kaohsiung, 811, Taiwan

    Shih-Yi Sheu

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  1. Wen-Ming Chen
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  2. Wei-Ting Chen
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Correspondence to Shih-Yi Sheu.

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Communicated by Erko Stackebrandt.

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The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence and the draft genome of Rheinheimera riviphila strain KYPC3T are LT555405 and SACS00000000. The Digital Protologue database Taxon Number for strain KYPC3T is TA00708.

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Chen, WM., Chen, WT., Young, CC. et al. Rheinheimera riviphila sp. nov., isolated from a freshwater stream. Arch Microbiol 201, 919–926 (2019). https://doi.org/10.1007/s00203-019-01657-5

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  • DOI: https://doi.org/10.1007/s00203-019-01657-5

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