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Quartz crystal microbalance immunosensor for the detection of antibodies to double-stranded DNA

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Abstract

We report the development of a novel quartz crystal microbalance immunosensor with the simultaneous measurement of resonance frequency and motional resistance for the detection of antibodies to double-stranded DNA (dsDNA). The immobilization of poly(l-lysine) and subsequent complexation with DNA resulted in formation of a sensitive dsDNA-containing nanofilm on the surface of a gold electrode. Atomic force microscopy has been applied for the characterization of a poly(l-lysine)–DNA film. After the blocking with bovine serum albumin, the immunosensor in flow-injection mode was used to detect the antibodies to dsDNA in purified protein solutions of antibodies to dsDNA and to single-stranded DNA, monoclonal human immunoglobulin G, DNase I and in blood serum of patients with bronchial asthma and systemic lupus erythematosus. Experimental results indicate high sensitivity and selectivity of the immunosensor.

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Acknowledgements

The support of Federal Center of Shared Facilities for Physicochemical Investigations of Substances and Materials (Kazan, Tatarstan) is gratefully acknowledged. This study is dedicated to the loving memory of Prof. Victor G. Vinter.

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Authors and Affiliations

  1. Department of Biochemistry, Kazan State University, Kremlevskaya 18, 420008, Kazan, Russia

    Rawil F. Fakhrullin, Victor G. Vinter, Alsu I. Zamaleeva, Maria V. Matveeva, Belayhun K. Temesgen, Dilara G. Ishmuchametova, Zinaida I. Abramova, Olga A. Konovalova & Myakzyum K. Salakhov

  2. Department of Optics and Nanophotonics, Kazan State University, Kremlevskaya 18, 420008, Kazan, Russia

    Olga A. Konovalova & Myakzyum K. Salakhov

  3. Department of Biology, Tatar State University of Humanities and Education, 420021, Kazan, Russia

    Roustem A. Kourbanov

Authors
  1. Rawil F. Fakhrullin
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  2. Victor G. Vinter
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  3. Alsu I. Zamaleeva
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  4. Maria V. Matveeva
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  5. Roustem A. Kourbanov
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  6. Belayhun K. Temesgen
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  7. Dilara G. Ishmuchametova
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  8. Zinaida I. Abramova
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  9. Olga A. Konovalova
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  10. Myakzyum K. Salakhov
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Corresponding author

Correspondence to Rawil F. Fakhrullin.

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In memoriam Prof. Victor G. Vinter

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Fakhrullin, R.F., Vinter, V.G., Zamaleeva, A.I. et al. Quartz crystal microbalance immunosensor for the detection of antibodies to double-stranded DNA. Anal Bioanal Chem 388, 367–375 (2007). https://doi.org/10.1007/s00216-007-1230-2

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  • DOI: https://doi.org/10.1007/s00216-007-1230-2

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