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Determination of total trans fats and oils by infrared spectroscopy for regulatory compliance

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Abstract

The mandatory requirement in many countries to declare the amount of trans fat present in food products and dietary supplements has led to a need for sensitive and accurate methodologies for the rapid quantitation of total trans fats and oils. Capillary gas chromatography (GC) and infrared spectroscopy (IR) are the two methods most commonly used to identify and quantify trans fatty acids for food labeling purposes (see the article by Delmonte and Rader in this ABC issue for a detailed presentation of GC methodology). The present article provides a comprehensive review of the IR technique and the current attenuated total reflection (ATR) Fourier-transform (FT) IR methodologies for the rapid determination of total trans fats and oils. This review also addresses potential sources of interferences and inaccuracies in FTIR determinations, particularly those done at low trans levels. Recent observations have shown that the presence of saturated fats caused interferences in the FTIR spectra observed for trans triacylglycerols. The recognition and resolution of previously unresolved quantitative issues improved the accuracy and sensitivity of the FTIR methodology. Once validated, it is anticipated that the new negative second-derivative ATR-FTIR procedure will make IR spectroscopy more suitable than ever, and a rapid alternative and/or complementary method to GC, for the rapid determination of total trans fats for regulatory compliance.

Infrared light bouncing inside an internal reflection crystal

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References

  1. Ratnayake WMN, Zehaluk C (2005) In: Akoh CC, Lai O-M (eds) Healthful lipids. AOCS Press, Champaign, IL, pp 1–32

    Google Scholar 

  2. Schrimpf-Moss J, Wilkening V (2005) In: Kodali DR, List GR (eds) Trans fats alternatives. AOCS Press, Champaign, IL, pp 26–33

    Google Scholar 

  3. Department of Health and Human Services, FDA (2003) Food labeling; trans fatty acids in nutrition labeling; nutrient content claims, and health claims; final rule. Fed Reg 68(133):41434–41506

    Google Scholar 

  4. Kodali DR, List GR (eds) (2005) Trans fats alternatives. AOCS Press, Champaign, IL

    Google Scholar 

  5. Mossoba MM, Kramer JKG, Delmonte P, Yurawecz MP, Rader JI (2005) In: Kodali DR, List GR (eds) Trans fats alternatives. AOCS Press, Champaign, IL, pp 47–70

    Google Scholar 

  6. Milosevic M, Milosevic V, Kramer JKG, Azizian H, Mossoba MM (2004) Lipid Technol 16:252–255

    CAS  Google Scholar 

  7. Mossoba MM, Kramer JKG, Milosevic V, Milosevic M, Azizian H (2007) J Am Oil Chem Soc (in press) DOI 10.1007/s11746-007-1038-4

  8. Mossoba MM, Yurawecz MP, McDonald RE (1996) J Am Oil Chem Soc 73:1003–1009

    Article  CAS  Google Scholar 

  9. Firestone D (ed) (1999) Official method Cd 14d-99. In: Official methods and recommended practices, 5th edn. American Oil Chemists’ Society, Champaign, IL

  10. Adam M, Mossoba MM, Lee T (2000) J Am Oil Chem Soc 77:457–462

    Article  CAS  Google Scholar 

  11. AOAC (2000) Method 2000.10. In: Official methods of analysis, 17th edn. AOAC International, Gaithersburg, MD

  12. Mossoba MM, Adam M, Lee T (2001) J AOAC Int 84:1144–1150

    Google Scholar 

  13. Firestone D, Sheppard A (1992) In: Christie WW (ed) Lipid methodology-one. The Oily Press, Ayr, UK, pp 273–322

    Google Scholar 

  14. Firestone D, LaBouliere P (1965) J Assoc Off Anal Chem 48:437–443

    CAS  Google Scholar 

  15. Mossoba MM, Kramer JKG, Fritsche J, Yurawecz MP, Eulitz K, Ku Y, Rader JI (2001) J Am Oil Chem Soc 78:631–634

    Article  CAS  Google Scholar 

  16. Christy AA, Egeberg PK, Ostensen ET (2003) Vibr Spectrosc 33:37–48

    Article  CAS  Google Scholar 

  17. Azizian H, Kramer JKG (2005) Lipids 40:855–867

    Article  CAS  Google Scholar 

  18. Azizian H, Ramalian AR, Winsborough SL, Kramer JKG, Hernadez M, Mossoba MM (2004) Lipid Technol 16:229–231

    CAS  Google Scholar 

  19. Firestone D (ed) (1999) Official Method Cd 14-95. In: Official methods and recommended practices, 5th edn. American Oil Chemists’ Society, Champaign, IL

  20. AOAC (1997) Method 965.34. In: Official methods of analysis, 17th edn. AOAC International, Gaithersburg, MD

  21. Harrick NJ (1967) Internal reflection spectroscopy. Wiley-Interscience, New York

  22. Mirabella FM (ed) (1992) Internal reflection spectroscopy (Practical Spectroscopy Series, vol. 15). Marcel Dekker, New York

  23. Ismail AA, Nicodemo A, Sedman J, van de Voort FR, Holzbauer IE (1998) In: Hamilton RJ, Cast J (eds) Spectral properties of lipids. Sheffield Academic Press/CRC Press, Boca Raton, FL, pp 235–269

    Google Scholar 

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Authors and Affiliations

  1. Food and Drug Administration, Center for Food Safety and Applied Nutrition, Mail Stop HFS-717, Room BE-012, 5100 Paint Branch Parkway, College Park, MD, 20740-3835, USA

    M. M. Mossoba

  2. MeV Photonics, Westport, CT, 06880, USA

    V. Milosevic & M. Milosevic

  3. Agriculture and Agri-Food Canada, Guelph, ON, N16 5C9, Canada

    J. K. G. Kramer

  4. NIR Technologies, Oakville, ON, L6M 2M2, Canada

    H. Azizian

Authors
  1. M. M. Mossoba
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  2. V. Milosevic
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  3. M. Milosevic
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  4. J. K. G. Kramer
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  5. H. Azizian
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Correspondence to M. M. Mossoba.

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Mossoba, M.M., Milosevic, V., Milosevic, M. et al. Determination of total trans fats and oils by infrared spectroscopy for regulatory compliance. Anal Bioanal Chem 389, 87–92 (2007). https://doi.org/10.1007/s00216-007-1262-7

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  • DOI: https://doi.org/10.1007/s00216-007-1262-7

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