Skip to main content
SpringerLink
Log in

Monitoring of cell cultures with LTCC microelectrode array

  • Original Paper
  • Published:
Analytical and Bioanalytical Chemistry Aims and scope Submit manuscript

Abstract

Monitoring of cell cultures in microbioreactors is a crucial task in cell bioassays and toxicological tests. In this work a novel tool based on a miniaturized sensor array fabricated using low-temperature cofired ceramics (LTCC) technology is presented. The developed device is applied to the monitoring of cell-culture media change, detection of the growth of various species, and in toxicological studies performed with the use of cells. Noninvasive monitoring performed with the LTCC microelectrode array can be applied for future cell-engineering purposes.

Microelectrode array for monitoring of cell cultures

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7
Fig. 8

Similar content being viewed by others

References

  1. Albrecht DR, Underhill GH, Wassermann TB, Sah RL, Bhatia SN (2006) Nat Methods 3:369–375

    Article  CAS  Google Scholar 

  2. Fernyhough ME, Hausman GJ, Guan LL, Okine E, Moore SS, Dodson MV (2008) Biochem Biophys Res Commun 368:455–457

    Article  CAS  Google Scholar 

  3. Trounson A, Elefanty A (2007) Curr Opin Biotechnol 18:432–433

    Article  CAS  Google Scholar 

  4. Lodish H, Berk A, Zipursky SL, Matsudaira P, Baltimore D, Darnell JE (1999) Molecular cell biology. W.H, 4th edn. Freeman, New York

  5. Giselbrecht S, Gietzelt T, Gottwald E, Trautmann C, Truckenmuller R, Weibezahn KF, Welle A (2006) Biomed Microdevices 8:191–199

    Article  CAS  Google Scholar 

  6. Girard PP, Cavalcanti-Adam EA, Kemkemer R, Spatz JP (2007) Soft Matter 3:307–326

    Article  CAS  Google Scholar 

  7. Andersson H, Van den Berg A (2003) Sens Actuators B 92:315–325

    Article  Google Scholar 

  8. Griffith LG, Swartz MA (2006) Nature Rev Mol Cell Biol 7:211–224

    Article  CAS  Google Scholar 

  9. Kim L, Toh YC, Voldman J, Yu H (2007) Lab Chip 7:681–694

    Article  CAS  Google Scholar 

  10. Leclerc E, Sakai Y, Fujii T (2004) Biotechnol Prog 20:750–755

    Article  CAS  Google Scholar 

  11. Chung BG, Flanagan LA, Rhee SW, Schwartz PH, Lee AP, Monuki ES, Jeon NL (2005) Lab Chip 5:401–406

    Article  CAS  Google Scholar 

  12. Tanaka Y, Sato K, Shimizu T, Yamato M, Okano T, Kitamori T (2007) Biosens Bioelectron 23:449–458

    Article  CAS  Google Scholar 

  13. Yi C, Yi CW, Ji S, Yang M (2006) Anal Chim Acta 560:1–23

    Article  CAS  Google Scholar 

  14. Starly B, Choubey A (2008) Ann Biomed Eng 36:30–40

    Article  Google Scholar 

  15. Ciosek P, Zawadzki K, Stadnik D, Bembnowicz P, Golonka L, Wróblewski W (2009) J Solid State Electrochem 13:129–135

    Article  CAS  Google Scholar 

  16. Ciosek P, Augustyniak E, Wróblewski W (2004) Analyst 129:639–644

    Article  CAS  Google Scholar 

  17. Ciosek P, Wróblewski W (2006) Talanta 69:1156–1161

    Article  CAS  Google Scholar 

  18. Ciosek P, Wróblewski W (2008) Talanta 76:548–556

    Article  CAS  Google Scholar 

  19. Ciosek P, Grabowska I, Brzózka Z, Wróblewski W (2008) Microchim Acta 163:139–145

    Article  CAS  Google Scholar 

  20. Ciosek P, Pokorska B, Romanowska E, Wróblewski W (2006) Electroanalysis 18:1266–1272

    Article  CAS  Google Scholar 

  21. Ciosek P, Mamińska R, Dybko A, Wróblewski W (2007) Sens Actuators B 127:8–14

    Article  Google Scholar 

  22. Habara M, Ikezaki H, Toko K (2004) Biosens Bioelectron 19:1559–1563

    Article  CAS  Google Scholar 

  23. Bao N, Wang J, Lu C (2008) Anal Bioanal Chem 391:933–942

    Article  CAS  Google Scholar 

  24. Ehrfeld W (2003) Electrochim Acta 48:2857–2868

    Article  CAS  Google Scholar 

  25. Golonka LJ, Roguszczak H, Zawada T, Radojewska J, Grabowska I, Chudy M, Dybko A, Brzozka Z, Stadnik D (2005) Sens Actuators B 111/112:396–402

    Article  Google Scholar 

Download references

Acknowledgment

This work was financially supported by the Ministry of Science and Higher Education, project no. N204 106 31/2448 (2006–2008). The authors thank Mr Piotr Żbikowski for assistance in LTCC structure fabrication. Patrycja Ciosek wishes to thank The Foundation for Polish Science for financial support.

Author information

Authors and Affiliations

  1. Warsaw University of Technology, Faculty of Chemistry, Noakowskiego 3, 00-664, Warsaw, Poland

    P. Ciosek, K. Zawadzki, J. Łopacińska, M. Skolimowski, Z. Brzózka & W. Wróblewski

  2. Faculty of Microsystem Electronics and Photonics, Wroclaw University of Technology, Wybrzeze Wyspianskiego 27, 50-370, Wroclaw, Poland

    P. Bembnowicz & L. J. Golonka

Authors
  1. P. Ciosek
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. K. Zawadzki
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. J. Łopacińska
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. M. Skolimowski
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. P. Bembnowicz
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. L. J. Golonka
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Z. Brzózka
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. W. Wróblewski
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to P. Ciosek.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Ciosek, P., Zawadzki, K., Łopacińska, J. et al. Monitoring of cell cultures with LTCC microelectrode array. Anal Bioanal Chem 393, 2029–2038 (2009). https://doi.org/10.1007/s00216-009-2651-x

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00216-009-2651-x

Keywords

Search

Navigation