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TLC surface integrity affects the detection of alkali adduct ions in TLC-MALDI analysis

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Abstract

Direct coupling of thin-layer chromatography (TLC) with matrix-assisted laser desorption ionization (MALDI) mass spectrometry allows fast and detailed characterization of a large variety of analytes. The use of this technique, however, presents great challenges in semiquantitative applications because of the complex phenomena occurring at the TLC surface. In our laboratory, we recently observed that the ion intensities of several alkali adduct ions were significantly different between the top and interior layer of the TLC plate. This indicates that the integrity of the TLC surface can have an important effect on the reproducibility of TLC- MALDI analyses.

MALDI imaging reveals that surface integrity affects the detection of alkali adductions in TLC-MALDI.

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Authors and Affiliations

  1. Biostatistics and Data Management, Research and Innovation Centre – Fondazione Edmund Mach, Via Edmund Mach, 138010, San Michele all’Adige, TN, Italy

    Yonghui Dong & Pietro Franceschi

  2. Bioorganic Chemistry Laboratory, Department of Physics, University of Trento, Via Sommarive 14, 38123, Povo, TN, Italy

    Yonghui Dong, Ruggero Ferrazza, Andrea Anesi & Graziano Guella

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  1. Yonghui Dong
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  2. Ruggero Ferrazza
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  3. Andrea Anesi
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  4. Graziano Guella
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  5. Pietro Franceschi
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Correspondence to Pietro Franceschi.

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Dong, Y., Ferrazza, R., Anesi, A. et al. TLC surface integrity affects the detection of alkali adduct ions in TLC-MALDI analysis. Anal Bioanal Chem 409, 5661–5666 (2017). https://doi.org/10.1007/s00216-017-0501-9

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  • DOI: https://doi.org/10.1007/s00216-017-0501-9

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