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Reference method for off-line analysis of nitrogen oxides in cell culture media by an ozone-based chemiluminescence detector

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Abstract

Nitric oxide (NO) and its by-products are important biological signals in human physiology and pathology particularly in the vascular and immune systems. Thus, in situ determination of the NO-related molecule (NOx) levels using embedded sensors is of high importance particularly in the context of cellular biocompatibility testing. However, NOx analytical reference method dedicated to the evaluation of biomaterial biocompatibility testing is lacking. Herein, we demonstrate a PAPA-NONOate-based reference method for the calibration of NOx sensors. After, the validation of this reference method and its potentialities were demonstrated for the detection of the oxidative stress-related NO secretion of vascular endothelial cells in a 3D tissue issued from 3D printing. Such NOx detection method can be an integral part of cell response to biomaterials.

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Funding

PANBioRA project has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement No 760921.

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Authors and Affiliations

  1. Univ. Grenoble Alpes, CEA, LETI, DTBS, L2CB, Grenoble, F-38000, France

    Ayman Chmayssem, Karen Monsalve-Grijalba, Mélanie Alias, Véronique Mourier, Séverine Vignoud & Pascal Mailley

  2. Inserm UMR 1121, 11 rue Humann, 67085, Strasbourg, France

    Loïc Scomazzon, Céline Muller & Julien Barthes

  3. Spartha Medical, 14B Rue de la Canardière, 67100, Strasbourg, France

    Nihal Engin Vrana

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  1. Ayman Chmayssem
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Chmayssem, A., Monsalve-Grijalba, K., Alias, M. et al. Reference method for off-line analysis of nitrogen oxides in cell culture media by an ozone-based chemiluminescence detector. Anal Bioanal Chem 413, 1383–1393 (2021). https://doi.org/10.1007/s00216-020-03102-9

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  • DOI: https://doi.org/10.1007/s00216-020-03102-9

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