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Antenna effect of pyridoxal phosphate on the fluorescence of mitoxantrone-silicon nanoparticles and its application in alkaline phosphatase assay

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Abstract

As a kind of sensing and imaging fluorescent probe with the merit of low toxicity, good stability, and environment-friendly, silicon nanoparticles (SiNPs) are currently attracting extensive research. In this work, we obtained mitoxantrone-SiNPs (MXT-SiNPs) with green emission by one-pot synthesis under mild temperature condition. The antenna based on pyridoxal phosphate (PLP) was designed for light-harvesting to enhance the luminescence of MXT-SiNPs and to establish a novel sensing strategy for alkaline phosphatase (ALP). PLP transfers the absorbed photon energy to MXT-SiNPs by forming Schiff base. When PLP is dephosphorized by ALP, the released free hydroxyl group reacts with aldehyde group to form internal hemiacetal, which leads to the failure of Schiff base formation. Based on the relationship between antenna formation ability and PLP hydrolysis degree, the activity of ALP can be measured. A good linear relationship was obtained from 0.2 to 3.0 U/L, with a limit of detection of 0.06 U/L. Furthermore, the sensing platform was successfully used to detect ALP in human serum with recovery of 97.6–106.2%. The rational design of antenna elements for fluorescent nanomaterials can not only provide a new pathway to manipulate the luminescence, but also provide a new direction for fluorescence sensing strategy.

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Funding

We received the financial support of the National Natural Science Foundation of China (81772287), the Natural Science Foundation of Fujian Province (2020J011203), the Program for Innovative Leading Talents in Fujian Province (2016B016), and the Program for Innovative Research Team in Science and Technology in Fujian Province University (2018B033).

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Author notes

  1. Hao-Hua Deng and Hui-Jing Yang contributed equally to this work.

Authors and Affiliations

  1. Fujian Key Laboratory of Drug Target Discovery and Structural and Functional Research, School of Pharmacy, Fujian Medical University, Fuzhou, 350004, Fujian, China

    Hao-Hua Deng, Hui-Jing Yang, Kai-Yuan Huang, Yi-Jing Zheng, Ying-Ying Xu, Hua-Ping Peng & Wei Chen

  2. School of Clinical Medicine, Fujian Medical University, Fuzhou, 350004, Fujian, China

    Hui-Jing Yang & Guo-Lin Hong

  3. Department of Laboratory Medicine, Fuzhou Second Hospital, Fuzhou, 350007, Fujian, China

    Yin-Huan Liu

  4. Department of Laboratory Medicine, Xiamen Key Laboratory of Genetic Testing, the First Affiliated Hospital of Xiamen University, Xiamen, 361003, China

    Guo-Lin Hong

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  1. Hao-Hua Deng
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Deng, HH., Yang, HJ., Huang, KY. et al. Antenna effect of pyridoxal phosphate on the fluorescence of mitoxantrone-silicon nanoparticles and its application in alkaline phosphatase assay. Anal Bioanal Chem 414, 4877–4884 (2022). https://doi.org/10.1007/s00216-022-04110-7

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