Skip to main content
SpringerLink
Log in

Application of ion exclusion chromatography (IEC) for the determination of sugar and carboxylic acids in hydrolysates from carbohydrate containing residues

  • Original Paper
  • Environmental Analysis
  • Published:
Fresenius' Journal of Analytical Chemistry Aims and scope Submit manuscript

Abstract

The oxidation of carbohydrate containing biomass residues by nitric acid yields several organic acids, especially sugar acids. To improve existing methods for the separation of such substance combinations by ion exclusion chromatography, the influence of temperature and eluant (proton) concentration on the retention behaviour and separation of 31 analytes belonging to substance groups like sugar acids, lactones, hydroxy-, mono- and polycarboxylic acids was checked with a Merck cation exchange column “Polyspher OA-HY”. By a combination of two Chromatographic parameter sets the Chromatographic versatility could be enhanced. The developed Chromatographic method is a useful tool for the analytical characterisation of organic acids in hydrolysates of biomass residues.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Fischer K, Bipp H-P, Riemschneider P, Bieniek D, Kettrup A (1994) Entwicklung von Methoden zur Dekontamination schwermetallbelasteter Böden und Altlasten mittels natürlicher organischer Komplexbildner. In: Wilderer PA, Potzel U, Rehbein V (eds) “Bay FORREST” Berichtsheft 2:219–224

  2. Sawyer TD (1964) Chem Rev 64: 633

    Article  CAS  Google Scholar 

  3. Escandar GM, Scala LF (1992) Can J Chem 70: 2053

    Article  CAS  Google Scholar 

  4. Charles DF (1981) Int Sugar J 83: 195

    CAS  Google Scholar 

  5. Clarke MA, Tsang WSC (1984) Int Sugar J 86: 215

    CAS  Google Scholar 

  6. Blake JD, Clarke MA, Richards GN (1987) J Chromatogr 398: 265

    Article  CAS  Google Scholar 

  7. Hicks KB, Lim PC, Haas MJ (1985) J Chromatogr 319: 159

    Article  CAS  Google Scholar 

  8. Rajakylä E (1981) J Chromatogr 218: 695

    Article  Google Scholar 

  9. Hicks KB (1986) Carbohydr Res 145: 312

    Article  CAS  Google Scholar 

  10. Fischer K, Bipp H-P, Bieniek D, Kettrup A (1995) J Chromatogr A 706: 361–373

    Article  CAS  Google Scholar 

  11. Mehltretter CL (1948) D-Saccarinic acid. US P 2,436,659, Feb 24, 1948

  12. Hachihama Y, Fujita H (1935) Studien zur Verwendung von Rohrzucker und Stärke. J Soc Chem Ind Japan [Suppl] 38: 744 B-745 B (Dez. 1935), Osaka University

    Google Scholar 

  13. Pal J, Saha AN (1953) J Indian Chem Soc, Ind News 16: 47

    CAS  Google Scholar 

  14. US P 2419038 (1947) Atlas Powder, Erf: Sanders MT; US P 2419019 (1947), Atlas Powder, Erf: Hales RA

  15. US P 2332915 (1942) General Chem., Erf: Brocks MJ

Download references

Author information

Authors and Affiliations

  1. GSF-Forschungszentrum für Umwelt und Gesundheit, GmbH, Institut für Ökologische Chemie, Neuherberg, Ingolstädter Landstrasse 1, D-85758, Oberschleißheim, Germany

    H. P. Bipp & D. Bieniek

  2. Lehrstuhl für Ökologische Chemie und Umweltanalytik, Institut für Chemie, Technische Universität München, D-85350, Freising-Weihenstephan, Germany

    K. Fischer & A. Kettrup

Authors
  1. H. P. Bipp
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. K. Fischer
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. D. Bieniek
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. A. Kettrup
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Presented on the ANAKON ’95 as poster

Rights and permissions

Reprints and permissions

About this article

Cite this article

Bipp, H.P., Fischer, K., Bieniek, D. et al. Application of ion exclusion chromatography (IEC) for the determination of sugar and carboxylic acids in hydrolysates from carbohydrate containing residues. Z. Anal. Chem. 357, 321–325 (1997). https://doi.org/10.1007/s002160050161

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s002160050161

Keywords

Search

Navigation