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Acrylamide determination in baked potatoes by HPLC–MS: effect of steam and correlation with colour indices

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Abstract

Acrylamide is a toxic compound and a thermal marker of food, deriving from Maillard reaction. A recent European Regulation established mitigation measures and benchmark levels for its reduction in many products encouraging the use of colourimetric scales providing a statistical correlation between colour intensity and acrylamide content. This study was focused on acrylamide determination by liquid chromatography coupled to mass spectrometry in baked potato samples cooked at different time, temperature and steam conditions. Effect of steam on acrylamide formation has been evaluated. Portions of cooked product characterized by different colours were sampled to create a colour scale. Acrylamide level was correlated with colour indices based on RGB channels. Results showed that similar colours, even obtained under different cooking conditions, were characterized by similar acrylamide levels. Statistical elaboration of the data allowed to find a high correlation between the two sets of data and to build a colour gradation that could be a starting point to elaborate a suitable tool to provide information on acrylamide content from colour analysis.

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References

  1. Taeymans D, Andersson A, Ashby P, Blank I, Gonde P, Van Eijck P (2005) Acrylamide: update on selected research activities conducted by the European food and drink industry. J AOAC Int 88:234–241

    CAS  PubMed  Google Scholar 

  2. Kumar J, Das S, Teoh SL (2018) Dietary acrylamide and the risk of developing cancer: facts to ponder. Front Nutr 5:1–14

    Article  Google Scholar 

  3. Habermann CE (1991) Kirk-Othmer encyclopedia of chemical technology. In: Kroschwizt JJ, Howe-Grant JM (eds) Acrylamide, 4th edn. vol 1. Wiley, New York, pp. 251–266

    Google Scholar 

  4. Casado JF, Montano A, Carle R (2014) Contribution of peptides and polyphenols from olive water to acrylamide formation in sterilized table olives. LWT-Food Sci Technol 59:376–382

    Article  CAS  Google Scholar 

  5. Mottram DS, Wedzicha BL, Dodson AT (2002) Food chemistry: acrylamide is formed in the Maillard reaction. Nature 419:448–449

    Article  CAS  PubMed  Google Scholar 

  6. Surdyk N, Rosean J, Andersson R, Aman P (2004) Effects of asparagine, fructose, and baking conditions on acrylamide content in yeast-leavened wheat bread. J Agr Food Chem 52:2047–2051

    Article  CAS  Google Scholar 

  7. Amrein TM, Andres L, Schonbachler B, Conde-Petit B, Escher F, Amado R (2005) Acrylamide in almond products. Eur Food Res Technol 221:14–18

    Article  CAS  Google Scholar 

  8. Becalski A, Lau BP, Lewis D, Seaman SW, Hayward S, Sahagian M, Ramesh M, Leclerc Y (2004) Acrylamide in French fries: influence of free amino acids and sugars. J Agr Food Chem 52:3801–3806

    Article  CAS  Google Scholar 

  9. FoodDrinkEurope (2019) Acrylamide Toolbox. web site: https://www.fooddrinkeurope.eu/uploads/publications_documents/FoodDrinkEurope_Acrylamide_Toolbox_2019.pdf

  10. Zyzak DV, Sanders RA, Stojanovic M, Tallmadge DH, Eberhart BL, Ewald DK (2003) Acrylamide formation mechanism in heated foods. J Agr Food Chem 51:4782–4787

    Article  CAS  Google Scholar 

  11. Biedermann M, Grob K (2003) Model study on acrylamide formation in potato, wheat flour and corn starch; ways to reduce acrylamide contents in bakery ware. Mitt Lebensm Hyg 94:406–422

    CAS  Google Scholar 

  12. Pedreschi F, Kaack K, Granby K (2004) Reduction of acrylamide formation in potato slices during frying. LWT-Food Sci Technol 37:679–685

    Article  CAS  Google Scholar 

  13. Robert F, Vuataz G, Pollien P, Saucy F, Alonso MI, Bauwens I, Blank I (2005) Acrylamide formation from asparagine under low moisture maillard reaction conditions. 2. crystalline vs amorphous model systems. J Agr Food Chem 53:4628–4632

    Article  CAS  Google Scholar 

  14. Elmore JS, Koutsidis G, Dodson AT, Mottram DS, Wedzicha BL (2005) Measurement of acrylamide and its precursors in potato, wheat, and rye model systems. J Agr Food Chem 53:1286–1293

    Article  CAS  Google Scholar 

  15. Taeymans D, Wood J, Ashby P, Blank I, Alfred S, Stadler RH, Gondè P, Eijck P, Lalljie S, Lingnert H, Lindblom M, Marissek R, Muller D, Tallmadge D, O’Brien J, Thompson S, Silvani D, Whitmore T (2004) A review of acrylamide: an industry perspective on research, analysis, formation, and control. Crit Rev Food Sci Nutr 44:323–347

    Article  CAS  PubMed  Google Scholar 

  16. Taubert D, Harlfinger S, Henkes L, Berkels R, Schomig E (2004) Influence of processing parameters on acrylamide formation during frying of potatoes. J Agr Food Chem 52:2735–2739

    Article  CAS  Google Scholar 

  17. Lingnert H, Jägerstad SG, Skog M, Kerstin LU, Törnqvist M, Åman P (2002) Acrylamide in food: mechanism of formation and influencing factors during heating of foods. Scand J Food Nutr 46:159–172

    Article  Google Scholar 

  18. Elder VA (2005) Method for enhancing acrylamide decomposition. Patent US20050118322

  19. Graf M, Amrein TM, Graf S, Szalay R, Escher F, Amadò R (2006) Reducing the acrylamide content of a semi-finished biscuit on industrial scale. LWT-Food Sci Technol 39:724–728

    Article  CAS  Google Scholar 

  20. Levine RA, Smith RE (2005) Sources of variability of acrylamide levels in a cracker model. J Agr Food Chem 53:4410–4416

    Article  CAS  Google Scholar 

  21. Jung MY, Choi DS, Ju JW (2003) A novel technique for limitation of acrylamide formation in fried and baked corn chips and in French fries. J Food Sci 68:1287–1290

    Article  CAS  Google Scholar 

  22. Zhang Y, Zhang Y (2008) Effect of natural antioxidants on kinetic behaviour of acrylamide formation and elimination in low-moisture asparagine-glucose model system. J Food Eng 85:105–115

    Article  CAS  Google Scholar 

  23. Zeng X, Cheng KW, Jiang Y, Lin ZX, Shi JJ, Ou SY, Chen F, Wang M (2009) Inhibition of acrylamide formation by vitamins in model reactions and fried potato strips. Food Chem 116:34–39

    Article  CAS  Google Scholar 

  24. IARC (1994) IARC Monographs on the Evaluations of Carcinogenic Risks to Humans. Some Industrial Chemicals. Acrylamide. Summary of data reported and Evaluation, vol 60. Lyon

  25. FAO/WHO (Joint FAO/WHO Expert Committee on Food Additives) (2006) Evaluation of certain Food Contaminants. Sixty-fourth report of the Joint FAO/WHO Expert Committee on Food Additives (Rome, 8–17 February 2005) WHO Technical Reports Series 930

  26. European Commission Recommendation of 3 May 2007 on the monitoring of acrylamide levels in food. OJEU, L 123/33

  27. European Commission Recommendation of 2 June 2010 on the monitoring of acrylamide levels in food. OJEU, L 137/4

  28. EFSA Panel on Contaminants in the Food Chain (CONTAM) (2015) Scientific Opinion on acrylamide in food. EFSA J 6(4104):1–321

    Google Scholar 

  29. European Commission Regulation 2017/2158 of 20 November 2017 establishing mitigation measures and benchmark levels for the reduction of the presence of acrylamide in food. OJEU, L 304/24

  30. Bignardi C, Cavazza A, Rinaldi M, Corradini C, Massini R (2013) Evaluation of thermal treatment markers in wheat flour-derived products cooked in conventional and in low-emissivity ovens. Food Chem 140:748–754

    Article  CAS  PubMed  Google Scholar 

  31. Pedreschi F, Kaack K, Granby K (2006) Acrylamide content and colour development in fried potato strips. Food Res Int 39:40–46

    Article  CAS  Google Scholar 

  32. Pedreschi F, León J, Mery D, Moyano P, Pedreschi R, Kaack K, Granby K (2007) Color development and acrylamide content of pre-dried potato chips. J Food Eng 79:786–793

    Article  CAS  Google Scholar 

  33. Gokmen V, Senyuva HZ (2006) Study of colour and acrylamide formation in coffee, wheat flour and potato chips during heating. Food Chem 99:238–243

    Article  CAS  Google Scholar 

  34. Mogol BA, Gokmen V (2014) Computer vision-based analysis of foods: a non-destructive colour measurement tool to monitor quality and safety. J Sci Food Agric 94:1259–1263

    Article  CAS  PubMed  Google Scholar 

  35. Hu Q, Xu X, Fu Y, Li Y (2015) Rapid methods for detecting acrylamide in thermally processed foods: a review. Food Control 56:135–146

    Article  CAS  Google Scholar 

  36. Calbiani F, Careri M, Elviri M, Mangia A, Zagnoni I (2004) Development and single-laboratory validation of a reversed.phase liquid chromatograpy-electrospray-tandem mass spectrometry method for identification and determination of acrylamide in food. J AOAC Int 87:101–115

    Google Scholar 

  37. N’Dri D, Calani L, Mazzeo T, Scazzina F, Rinaldi M, Del Rio D, Pellegrini N, Brighenti F (2010) Effects of different maturity stages on antioxidant content of Ivorian Gnagnan (Solanum indicum L.) berries. Molecules 15:7125–7138

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  38. EURACHEM Guide (1998) The fitness for purpose of analytical methods: a laboratory guide to method validation and related topics. http://www.eurachem.org/

  39. Ahrné L, Ardersson C-G, Floberg P, Rosén J, Lingnert H (2007) Effect of crust temperature and water content on acrylamide formation during baking of white bread: steam and falling temperature baking. LWT-Food Sci Technol 40:1708–1715

    Article  CAS  Google Scholar 

  40. Romani S, Bacchiocca M, Rocculi P, Dalla Rosa M (2008) Influence of frying conditions on acrylamide content and other quality characteristics of French fries. J Food Compos Anal 22:582–588

    Article  CAS  Google Scholar 

  41. Ciesarova Z, Kiss E, Kolek E (2006) Study of factors affecting acrylamide levels in model systems. Czech J Food Sci 24:133–137

    Article  CAS  Google Scholar 

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Acknowledgements

The authors are thankful to Dr. Maria Mattera for her precious collaboration in the research coordination.

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Correspondence to Antonella Cavazza.

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Bignardi, C., Cavazza, A., Grimaldi, M. et al. Acrylamide determination in baked potatoes by HPLC–MS: effect of steam and correlation with colour indices. Eur Food Res Technol 245, 2393–2400 (2019). https://doi.org/10.1007/s00217-019-03357-2

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  • DOI: https://doi.org/10.1007/s00217-019-03357-2

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