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Understanding Responses of Soil Microbiome to the Nitrogen and Phosphorus Addition in Metasequoia glyptostroboides Plantations of Different Ages

  • Soil Microbiology
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Abstract

Nitrogen (N) and phosphorus (P) have significant effects on soil microbial community diversity, composition, and function. Also, trees of different life stages have different fertilization requirements. In this study, we designed three N additions and three P levels (5 years of experimental treatment) at two Metasequoia glyptostroboides plantations of different ages (young, 6 years old; middle mature, 24 years old) to understand how different addition levels of N and P affect the soil microbiome. Here, the N fertilization of M. glyptostroboides plantation land (5 years of experimental treatment) significantly enriched microbes (e.g., Lysobacter, Luteimonas, and Rhodanobacter) involved in nitrification, denitrification, and P-starvation response regulation, which might further lead to the decreasing in alpha diversity (especially in 6YMP soil). The P addition could impact the genes involved in inorganic P-solubilization and organic P-mineralization by increasing soil AP and TP. Moreover, the functional differences in the soil microbiomes were identified between the 6YMP and 24YMP soil. This study provides valuable information that improves our understanding on the effects of N and P input on the belowground soil microbial community and functional characteristics in plantations of different stand ages.

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Data Availability

The raw data of the sequences had been submitted to NCBI (accession number: PRJNA685784).

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Acknowledgements

We thank the worker in the Yellow Sea Park for the sample collection.

Funding

This work was supported by the National Natural Science Foundation of China (31770756) and the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD).

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Authors and Affiliations

  1. East China Coastal Forest Ecosystem Long-Term Research Station, Research Institute of Subtropical Forestry, Chinese Academy of Forestry, Hangzhou, 311400, Zhejiang, China

    Lei Wang, Yuxiang Wen, Ran Tong, Hui Zhang & Tonggui Wu

  2. College of Life Sciences, Nanjing Normal University, Wenyuan Road 1, Nanjing, 210046, China

    Lei Wang, Ting Hu & Lifeng Zhu

  3. State Key Laboratory of Lake Science and Environment, Nanjing Institute of Geography and Limnology, Chinese Academy of Sciences, Nanjing, China

    Ting Hu & Jianjun Wang

  4. Mingke Biotechnology Co., Ltd, Hangzhou, China

    Hua Chen & Guoqi Liu

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  1. Lei Wang
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Contributions

TW, JW, and LZ conceived the project. LW, YX, RT, and HZ collected the samples. LW performed the experiments. LW, HC, TH, and GL analyzed the data. LZ, TW, and JW wrote the manuscript. All authors approved the final version of the manuscript.

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Correspondence to Lifeng Zhu or Tonggui Wu.

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Wang, L., Wen, Y., Tong, R. et al. Understanding Responses of Soil Microbiome to the Nitrogen and Phosphorus Addition in Metasequoia glyptostroboides Plantations of Different Ages. Microb Ecol 84, 565–579 (2022). https://doi.org/10.1007/s00248-021-01863-z

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