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Development of a novel uric-acid-responsive regulatory system in Escherichia coli

  • Applied genetics and molecular biotechnology
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Abstract

A novel uric-acid-responsive regulatory system was developed in Escherichia coli by adapting the HucR-related regulatory elements from Deinococcus radiodurans into E. coli. The induction performance of this system was compared to the performance of both the pBAD and pET systems. Our novel regulatory system was induced in a dose-dependent manner in the presence of uric acid and exhibited low basal expression in its absence. The system was characterized by a wide dynamic range of induction, being compatible with various E. coli strains and not requiring genomic modifications of the bacterial host. E. coli DH5α and DH10B were the most suitable host strains for optimal performance of this system. In conclusion, we developed a regulatory system with potential for applications in both recombinant protein expression and metabolic optimization.

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Acknowledgments

The authors would like to thank Mr. Ye Mao for his technical assistance in this research. This work was supported by the Ministry of Science and Technology of China Grant 2013CB734003, the National Natural Science Foundation of China Grant 21172095, and the Key Research Program of the Chinese Academy of Sciences Grant KSZD-EW-Z-015-2.

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Authors and Affiliations

  1. CAS Key Laboratory of Microbial Physiological and Metabolic Engineering, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China

    Chaoning Liang, Dandan Xiong, Yi Zhang, Shanshan Mu & Shuang-Yan Tang

  2. University of Chinese Academy of Sciences, Beijing, 100009, China

    Dandan Xiong, Yi Zhang & Shanshan Mu

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  1. Chaoning Liang
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  2. Dandan Xiong
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  3. Yi Zhang
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  4. Shanshan Mu
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  5. Shuang-Yan Tang
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Correspondence to Shuang-Yan Tang.

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Chaoning Liang and Dandan Xiong contributed equally to this work.

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Liang, C., Xiong, D., Zhang, Y. et al. Development of a novel uric-acid-responsive regulatory system in Escherichia coli . Appl Microbiol Biotechnol 99, 2267–2275 (2015). https://doi.org/10.1007/s00253-014-6290-6

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  • DOI: https://doi.org/10.1007/s00253-014-6290-6

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