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Streptomyces tsukubaensis as a new model for carbon repression: transcriptomic response to tacrolimus repressing carbon sources

  • Genomics, transcriptomics, proteomics
  • Published:
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Abstract

In this work, we identified glucose and glycerol as tacrolimus repressing carbon sources in the important species Streptomyces tsukubaensis. A genome-wide analysis of the transcriptomic response to glucose and glycerol additions was performed using microarray technology. The transcriptional time series obtained allowed us to compare the transcriptomic profiling of S. tsukubaensis growing under tacrolimus producing and non-producing conditions. The analysis revealed important and different metabolic changes after the additions and a lack of transcriptional activation of the fkb cluster. In addition, we detected important differences in the transcriptional response to glucose between S. tsukubaensis and the model species Streptomyces coelicolor. A number of genes encoding key players of morphological and biochemical differentiation were strongly and permanently downregulated by the carbon sources. Finally, we identified several genes showing transcriptional profiles highly correlated to that of the tacrolimus biosynthetic pathway regulator FkbN that might be potential candidates for the improvement of tacrolimus production.

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Acknowledgements

This work was supported by the European Union through an ERA-IB (PIM2010EEI-00677) international cooperation project. M. Ordóñez-Robles received a FPU fellowship of the Ministerio de Educación y Ciencia (Spain). We thank Dr. C. Prieto for sharing their prediction results of intrinsic terminators that were included in the microarray probe design. We acknowledge the technical support of B. Martín, J. Merino, A. Casenave and A. Mulero (INBIOTEC).

Funding

This study was funded by the Government of Spain (grant number PIM2010-EEI00677). María Ordóñez-Robles received a FPU grant from the Government of Spain (grant number AP2009-4508).

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Author notes

  1. Fernando Santos-Beneit

    Present address: Centre for Bacterial Cell Biology, Institute for Cell and Molecular Biosciences, Medical School, Newcastle University, Newcastle upon Tyne, UK

  2. Silvia M. Albillos

    Present address: Departamento de Biotecnología y Ciencia de los Alimentos, Facultad de Ciencias, Universidad de Burgos, 09001, Burgos, Spain

Authors and Affiliations

  1. Área de Microbiología, Facultad de Ciencias Biológicas y Ambientales, Universidad de León, 24071, León, Spain

    María Ordóñez-Robles, Paloma Liras, Juan F. Martín & Antonio Rodríguez-García

  2. Instituto de Biotecnología de León, INBIOTEC, Avda. Real no. 1, 24006, León, Spain

    María Ordóñez-Robles, Fernando Santos-Beneit, Silvia M. Albillos, Paloma Liras, Juan F. Martín & Antonio Rodríguez-García

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  1. María Ordóñez-Robles
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  6. Antonio Rodríguez-García
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Correspondence to Antonio Rodríguez-García.

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Ordóñez-Robles, M., Santos-Beneit, F., Albillos, S.M. et al. Streptomyces tsukubaensis as a new model for carbon repression: transcriptomic response to tacrolimus repressing carbon sources. Appl Microbiol Biotechnol 101, 8181–8195 (2017). https://doi.org/10.1007/s00253-017-8545-5

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