Abstract
Cyanobacteria are photosynthetic prokaryotes that can fix atmospheric CO2 and can be engineered to produce industrially important compounds such as alcohols, free fatty acids, alkanes used in next-generation biofuels, and commodity chemicals such as ethylene or farnesene. They can be easily genetically manipulated, have minimal nutrient requirements, and are quite tolerant to abiotic stress making them an appealing alternative to other biofuel-producing microbes which require additional carbon sources and plants which compete with food crops for arable land. Many of the compounds produced in cyanobacteria are toxic as titers increase which can slow growth, reduce production, and decrease overall biomass. Additionally, many factors associated with outdoor culturing of cyanobacteria such as UV exposure and fluctuations in temperature can also limit the production potential of cyanobacteria. For cyanobacteria to be utilized successfully as biofactories, tolerance to these stressors must be increased and ameliorating stress responses must be enhanced. Genetic manipulation, directed evolution, and supplementation of culture media with antioxidants are all viable strategies for designing more robust cyanobacterial strains that have the potential to meet industrial production goals.
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Abbreviations
- CO2 :
-
carbon dioxide
- UV:
-
ultraviolet light
- UVR:
-
ultraviolet radiation
- PAR:
-
photosynthetically active radiation
- ROS:
-
reactive oxygen species
- RuBisCO:
-
ribulose-1,5-bisphosphate carboxylase/oxygenase
- PSII:
-
photosystem II
- HSR:
-
heat shock response
- FFA:
-
free fatty acid
- O2 − :
-
superoxide anion
- 1O2 :
-
singlet oxygen
- H2O2 :
-
hydrogen peroxide
- OH:
-
hydroxyl radical
- PSI:
-
photosystem I
- GB:
-
glycine betaine
- SOD:
-
superoxide dismutase
- SOR:
-
superoxide reductase
- NAC:
-
N-acetyl-l-cysteine
- DNA:
-
deoxyribonucleic acid
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Support for some of the studies described in this review was provided by the National Science Foundation funded Emerging Frontiers in Research and Innovation grant no. 1332341.
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Kitchener, R.L., Grunden, A.M. Methods for enhancing cyanobacterial stress tolerance to enable improved production of biofuels and industrially relevant chemicals. Appl Microbiol Biotechnol 102, 1617–1628 (2018). https://doi.org/10.1007/s00253-018-8755-5
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DOI: https://doi.org/10.1007/s00253-018-8755-5