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Steviol glycosides profile in Stevia rebaudiana Bertoni hairy roots cultured under oxidative stress-inducing conditions

  • Applied microbial and cell physiology
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Abstract

The ability to synthesize particular steviol glycosides (SvGls) was studied in Stevia rebaudiana Bertoni hairy roots (HR) grown in the light or in the dark under the influence of different osmotic active compounds. Manipulation of culture conditions led to changes in the morphology and growth rate of HR, as well as to an increase in oxidative stress manifested as an enhancement in endogenous hydrogen peroxide concentration in the cultured samples. The highest level of H2O2 was noted in HR cultured under light or in the medium with the highest osmotic potential. This correlated with the highest increase in the expression level of ent-kaurenoic acid hydroxylase, responsible for the redirection of metabolic route to SvGls biosynthesis pathway. An analysis of transcriptional activity of some UDPglucosyltransferase (UGT85c2, UGT74g1, UGT76g1) revealed that all of them were upregulated due to the manipulation of culture conditions. However, the level of their upregulation depended on the type of stress factor used in our experiment. Analysis of SvGls content revealed that HR grown under all applied conditions were able to synthesize and accumulate several SvGls but their concentration differed between the samples across the different conditions. The level of rebaudioside A concentration exceeded the content of stevioside in HR in all tested conditions. Concomitantly, the presence of some minor SvGls, such as steviolbioside and rebaudioside F, was confirmed only in HR cultured in the lowest osmotic potential of the medium while rebaudioside D was also detected in the samples cultured in the media supplemented with NaCl or PEG.

Key Points

● Several steviol glycosides are synthesized in hairy roots of S. rebaudiana.

● Light or osmotic factors cause enhancement in oxidative stress level in hairy roots.

● It correlates with a significant increase in the level of KAH expression.

● UGTs expression and steviol glycosides content depends on culture conditions.

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All the relevant data used to support the findings of this study are included within the article.

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Acknowledgments

We would like to thank Barbara Coulter for English language corrections.

Funding

This work was supported in part by projects funded by the National Science Centre (no. 2012/05/B/NZ9/01035 and no. 2013/09/N/NZ9/01650.

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Authors and Affiliations

  1. The Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, ul. Niezapominajek 21, 30-239, Krakow, Poland

    Marta Libik-Konieczny, Żaneta Michalec-Warzecha & Michał Dziurka

  2. Department of Plant Cytology and Embryology, Jagiellonian University, Gronostajowa 9, 30-387, Krakow, Poland

    Olga Zastawny & Robert Konieczny

  3. Malopolska Centre of Biotechnology, Jagiellonian University, Gronostajowa 7A, 30-387, Krakow, Poland

    Piotr Rozpądek

  4. Department of Agriculture, Food and Environment, University of Pisa, Via del Borghetto 80, I-56124, Pisa, Italy

    Laura Pistelli

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  1. Marta Libik-Konieczny
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Contributions

MLK designed the experiment, ŻMW, PR, MD, OZ, RK, and LP performed the experiments and the data analysis. MLK prepared the manuscript. All authors approved the final version of the manuscript.

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Correspondence to Marta Libik-Konieczny.

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Libik-Konieczny, M., Michalec-Warzecha, Ż., Dziurka, M. et al. Steviol glycosides profile in Stevia rebaudiana Bertoni hairy roots cultured under oxidative stress-inducing conditions. Appl Microbiol Biotechnol 104, 5929–5941 (2020). https://doi.org/10.1007/s00253-020-10661-5

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  • DOI: https://doi.org/10.1007/s00253-020-10661-5

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