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Comparison Between Fluorescent Probe and Ion-Selective Electrode Methods for Intracellular pH Determination in Leuconostoc mesenteroides

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Abstract

The intracellular pH (pHin) of Leuconostoc mesenteroides subsp. mesenteroides 19D was evaluated by two different methods, fluorescent probe and ion-selective electrode. Two fluorescent probes 5 (and-6)-carboxyfluorescein diacetate succinimidyl ester (cFDASE) and 5 (and-6)-carboxy-2′,7′-dichlorofluorescein diacetate succinimidyl ester (cDCFDASE) were tested to evaluate the intracellular pH (pHin) of living cells at a medium pH (pHex) ranged from 5.0 to 6.5. Salicylic acid was used as a probe for the ion-selective electrode method. Cells kept 60–80% of cFDASE probe at all pHex values against 5–10% of cDCFDASE probe at pHex ≤ 6.0. The pHin values measured by the ion-selective electrode were higher by 0.1–0.6 pH units at pHex ranged from 5.0 to 6.5 than those determinated by fluorescent probe method. The possibility to study the intracellular pH at a wide external pH range using a single probe, and the simplicity of the material and experimental protocol may make the ion-selective electrode method most useful and easy to measure the intracellular pH of lactic acid bacteria compared with the other techniques like fluorescent probes.

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Authors and Affiliations

  1. Research Center for Redox Applications in Foods (RCRAF), Iğdır University, 76000, Iğdır, Turkey

    Duried Alwazeer

  2. LGDP Laboratory of Genomes and Plant Development, Université de Perpignan, Perpignan, France

    Christophe Riondet

  3. UMR Procédés Alimentaires et Microbiologiques A 02.102 AgroSup Dijon - Université de Bourgogne, 1 Esplanade Erasme, 21000, Dijon, France

    Rémy Cachon

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  1. Duried Alwazeer
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  2. Christophe Riondet
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  3. Rémy Cachon
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Correspondence to Duried Alwazeer.

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Alwazeer, D., Riondet, C. & Cachon, R. Comparison Between Fluorescent Probe and Ion-Selective Electrode Methods for Intracellular pH Determination in Leuconostoc mesenteroides. Curr Microbiol 75, 1493–1497 (2018). https://doi.org/10.1007/s00284-018-1550-9

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  • DOI: https://doi.org/10.1007/s00284-018-1550-9

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