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Flavobacterium sharifuzzamanii sp. nov., Isolated from the Sediments of the East China Sea

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Abstract

A novel bacterial strain A7.6T was isolated from the sediments collected near the Zhairuo Island located in the East China Sea and characterized using a polyphasic approach. Cells were Gram-stain-negative, rod-shaped, non-spore forming, non-flagellated but motile by gliding. The strain was aerobic, positive for oxidase and catalase activities. The strain can grow at 4–35 °C, pH 5.5–9.0, and 0–3% (w/v) NaCl concentration. The major polar lipid was phosphatidylethanolamine, the predominant fatty acids (> 10%) were iso-C15:0 and summed feature 3 (C16:1 ω7c and/or C16:1 ω6c). The genomic G+C content was 33.6 mol% and the major respiratory quinone was menaquinone 6. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain A7.6T belonged to the genus Flavobacterium and was closely related to Flavobacterium tistrianum GB 56.1T (98.4% similarity), F. nitrogenifigens NXU-44T (98.4%), F. ginsenosidimutans THG 01T (98.0%) and F. anhuiense D3T (97.7%). Average nucleotide identities and digital DNA–DNA hybridizations values for genomes ranged from 75.9 to 91.4% and 21.4 to 43.9% between strain A7.6T and its closest phylogenetic neighbors. The polyphasic characterization indicated that strain A7.6T represented a novel species of the genus Flavobacterium, for which the name Flavobacterium sharifuzzamanii is proposed. The type strain is A7.6T (= KCTC 62405T = MCCC 1K03485T). The NCBI GenBank accession number for the 16S rRNA gene of A7.6T is MH396692, and for the genome sequence is QJGZ00000000. The digital protologue database (DPD) Taxon Number is TA00643.

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Abbreviations

PE:

Phosphatidylethanolamine

ANI:

Average nucleotide identity

dDDH:

Digital DNA–DNA hybridization

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Acknowledgements

The work was supported by Natural Science Foundation of Zhejiang Province (LY18D060003 and LY18C060002), and Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, China (2016LMFS-B19). We thank Prof. Min Wu (Zhejiang University) for his technical support.

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Author notes

  1. Sanjit C. Debnath and Can Chen have contributed equally to this work.

Authors and Affiliations

  1. Ocean College, Zhejiang University, Zhoushan, People’s Republic of China

    Sanjit C. Debnath, Can Chen, Shu-Xia Liu, Ya-Nan Di, Dao-Qiong Zheng, Xin-Yang Li, Jin-Zhong Xu & Pin-Mei Wang

  2. Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao, People’s Republic of China

    Shu-Xia Liu

  3. Key Laboratory of Marine Ecosystem and Biogeochemistry, Second Institute of Oceanography, State Oceanic Administration, Hangzhou, 310012, People’s Republic of China

    Xue-Wei Xu

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  1. Sanjit C. Debnath
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Correspondence to Jin-Zhong Xu or Pin-Mei Wang.

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Additional information

The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain A7.6T is MH396692. The GenBank/EMBL/DDBJ accession numbers for the genome sequence of strain A7.6T, F. tistrianum KCTC 42679T, F. nitrogenifigens DSM 29982T, F. ginsenosidimutans KCTC 42980T are QJGZ00000000, QJRH00000000, QJRI00000000, and QJRJ00000000, respectively. The Digital protologue database (DPD) Taxon Number for strain A7.6T is TA00643.

Electronic supplementary material

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284_2018_1609_MOESM1_ESM.docx

Additional transmission electron micrograph, Table S1, the Maximum-Likelihood, Maximum-Parsimony phylogenetic tree based on 16S rRNA gene sequences and TLC figures of polar lipids of strain A7.6T are available as supplementary materials. Supplementary material 1 (DOCX 1677 KB)

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Debnath, S.C., Chen, C., Liu, SX. et al. Flavobacterium sharifuzzamanii sp. nov., Isolated from the Sediments of the East China Sea. Curr Microbiol 76, 297–303 (2019). https://doi.org/10.1007/s00284-018-1609-7

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  • DOI: https://doi.org/10.1007/s00284-018-1609-7

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