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Flavobacterium viscosus sp. nov. and Flavobacterium tangerina sp. nov., from Primates Feces

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Abstract

Strains YIM 102796T and YIM 102701-2T were isolated from the feces of Macaca mulatta and Hylobates hoolock, respectively, living in the Yunnan Wild Animal Park, Yunnan province of China. The two strains were Gram-stain-negative, non-gliding, produced flexirubin pigments, non-flagellated and aerobic bacteria. The 16S rRNA gene-based phylogenetic analysis indicate that both YIM 102796T and YIM 102701-2T are members of the genus Flavobacterium, closely related to F. ummariense DS-12T (95.9% similarity) and F. ceti 454-2T (93.8% similarity), respectively. The two strains shared 95.1 % 16S rRNA gene sequence similarity. The average nucleotide identity and digital DNA–DNA hybridization values between the two strains were 76.5% and 22.9%, respectively, indicating that they are separate species. DNA G+C contents of YIM 102796T and YIM 102701-2T were 32.3 mol% and 34.0 mol%, respectively. Strains are able to grow at 4–37 °C, at pH 7.0–8.0 and in 0–2% (w/v) NaCl. Predominant fatty acid constituents (>7 %) were iso-C15:0, summed feature 3 (C16 : 1ω6c and/or C16 : 1ω7c) and summed feature 9 (iso-C17:1ω9c and/or 10-methylC16:0). Menaquinone 6 is major respiratory quinone. The predominant polar lipids were very similar to each other, comprising phosphatidylethanolamine, and multiple unknown aminolipids and unidentified polar lipids, and an unidentified aminophospholipid. On the basis of phenotypic and phylogenetic distinctiveness, it is suggested that the two strains represent two novel Flavobacterium species with strain YIM 102796T (=KCTC 52101T=CCTCC AB 2016015T) as the type strain of Flavobacterium viscosus sp. nov. and strain YIM 102701-2T (=KCTC 52100T=CCTCC AB 2016028T) as the type strain of Flavobacterium tangerina sp. nov.

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Acknowledgements

We thank Dr. Fernández-Garayzábal for providing with the reference strains. This research was supported by the National Natural Science Foundation of China (Nos. 31270001 and 31460005).

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Authors and Affiliations

  1. State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan Institute of Microbiology, School of Life Sciences, Yunnan University, 2# North Cui-Hu Road, Kunming, Yunnan, 650091, People’s Republic of China

    Guiding Li, Xiu Chen, Songbiao Shi, Longqian Jiang, Yi Jiang & Chenglin Jiang

  2. Institute of Microbial Pharmaceuticals, Northeastern University, Shenyang, Liaoning, 110819, People’s Republic of China

    Guiding Li, Li Han & Xueshi Huang

  3. School of Medicine, Kunming University, Kunming, 650214, People’s Republic of China

    Xiu Chen

  4. Yunnan Wild Animal Park, Fengyuan Road, Panlong District, Kunming, Yunnan, 650224, People’s Republic of China

    Youlong Li

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  1. Guiding Li
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Correspondence to Yi Jiang.

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Nucleotide and Whole-Genome Sequence Accession Numbers

The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequences of strains YIM 102796T and YIM 102701-2T are MK184511 and MK184532, respectively. The GenBank/EMBL/DDBJ accession numbers for the whole-genome sequences of strains YIM 102796T and YIM 102701-2T are RQTJ00000000 and RQVQ00000000, respectively.

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Li, G., Chen, X., Li, Y. et al. Flavobacterium viscosus sp. nov. and Flavobacterium tangerina sp. nov., from Primates Feces. Curr Microbiol 76, 818–823 (2019). https://doi.org/10.1007/s00284-019-01692-x

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