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Molecular Characterization and Phylogeny of Taenia hydatigena and Echinococcus granulosus from Iranian Sheep and Cattle Based on COX1 Gene

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Abstract

Hydatid cyst, the larval stage of Echinococcus granulosus, and Cysticercus tenuicollis, the larval stage of Taenia hydatigena, are prevalent in domestic, livestock, and wild ruminants. The main goal of this research was to identify the isolates of E. granulosus and C. tenuicollis by partial sequencing with PCR amplification of the cytochrome C oxidase 1 (COX1) gene. During a routine veterinary inspection at a Chabahar city slaughterhouse, two samples of hydatid cysts from sheep's liver and cattle's lung and two samples of C. tenuicollis from sheep's liver were collected. After DNA extraction, the fragment of the COX1 gene was amplified by the PCR method. Sample sequences were modified and synchronized by Chromas and CLC genomic workbench 11 software. Sequence analysis was carried out by BLAST algorithms and GenBank databases. Phylogenetic trees were performed using MEGA 7 software and the neighbor-joining and maximum likelihood method for T. hydatigena and E. granulousus. The result indicated that the main genotype of parasites and the amplified fragment size were G1 and approximately 455 bp, respectively. The analysis of phylogenetic trees based on nucleic acid for four samples showed that there was a common ancestor. However, the shift in nucleotides in the two isolates in E. granulosus and the two isolates of T. hydatigena were non-synonymous type and synonymous type, respectively. The present study showed that the dominant genotype in all isolates was G1 and this report was similar to other studies in Iran and the world. Also, the partial COX1 gene sequence was matched with T. hydatigena.

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Acknowledgements

The authors express their appreciation and gratitude to all those who have directly or indirectly contributed to this project. This study is the result of helminthology project Dr. Soodabeh Etemadi which was done in Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences.

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Authors and Affiliations

  1. Department of Medical Parasitology and Mycology, Faculty of Public Health, Tehran University of Medical Sciences, Tehran, Iran

    Vahid Raissi

  2. Department of Medical Parasitology and Mycology, Faculty of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran

    Vahid Raissi

  3. Department of Medical Parasitology and Mycology, Faculty of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran

    Soudabeh Etemadi, Mehdi Shahraki & Alireza Salimi-Khorashad

  4. Infectious Diseases and Tropical Medicine Research Center, Resistant Tuberculosis Institute, Zahedan University of Medical Sciences, Zahedan, Iran

    Soudabeh Etemadi & Alireza Salimi-Khorashad

  5. Department of Medical Genetics, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran

    Nasrin Sohrabi

  6. Department of Parasitology, School of Allied Medical Sciences, Ilam University of Medical Sciences, Ilam, Iran

    Omid Raiesi

  7. Diagnostic and Research Unit of Parasitic Diseases (DRUP), Department of Medical Parasitology, Kasr Al-Ainy Faculty of Medicine, Cairo University, Cairo, Egypt

    Asmaa Ibrahim

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  1. Vahid Raissi
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Contributions

VR, AI, SE helped in study concept and design; NS, OR acquired the data; SE; MS, ASK analyzed and interpreted the data; VR and SE drafted and revised the manuscript.

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Correspondence to Soudabeh Etemadi.

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The authors and coauthors declare that they have no conflict of interest that affects this study.

Ethical Approval

All the studied samples have been used in this study based on ethical considerations determined by the relevant authorities (Veterinary Organization of Iran). Also, this study was the research project of Ms. Soodabeh Etemadi in the PhD course.

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Raissi, V., Etemadi, S., Sohrabi, N. et al. Molecular Characterization and Phylogeny of Taenia hydatigena and Echinococcus granulosus from Iranian Sheep and Cattle Based on COX1 Gene. Curr Microbiol 78, 1202–1207 (2021). https://doi.org/10.1007/s00284-021-02377-0

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