Abstract
Somatic embryos directly formed at cut edges or on the surface of leaf explants, around cut ends or along side surfaces of petiole and stem explants of ‘Golden Pothos’ [Epipremnum aureum (Linden & Andre) Bunt.] on Murashige and Skoog (MS) medium supplemented with N-(2-chloro-4-pyridyl)-N′-phenylurea (CPPU) or N-phenyl-N′-1, 2, 3-thiadiazol-5-ylurea (TDZ) with α-naphthalene acetic acid (NAA) and a medium called MK containing MS salts with Kao’s vitamins, supplemented with 2.0 mg/l TDZ and 0.2 mg/l NAA. Somatic embryos were also produced on MS medium containing 2.0 mg/l kinetin (KN) and 0.5 mg/l 2,4-dichlorophenoxyacetic acid (2,4-D) from leaf and petiole explants, MS medium supplemented with 2.0 mg/l CPPU and 0.5 mg/l 2,4-D from petiole and stem explants, and 2.0 mg/l TDZ and 0.2 mg/l or 0.5 mg/l 2,4-D from stem explants. In addition, somatic embryos occurred from stem explants on Chu’s N6 medium containing 2.0 mg/l CPPU and 0.2 mg/l NAA. Somatic embryos matured and grew into multiple buds, shoots, or even plantlets after 2–3 months on the initial culture medium. Germination was optimal on MS medium containing either 2 mg/l 6-benzylaminopurine (BA) and 0.2 mg/l NAA or 2 mg/l zeatin and 0.2 mg/l NAA. Shoots elongated better and roots developed well on MS medium with no growth regulators. Approximately 30–100 plantlets were regenerated from each explant. The regenerated plants grew vigorously after transplanting to a soil-less container substrate in a shaded greenhouse.
Similar content being viewed by others
Abbreviations
- BA:
-
6-Benzylaminopurine
- CPPU:
-
N-(2-Chloro-4-pyridyl)-N′-phenylurea
- 2,4-D:
-
2,4-Dichlorophenoxyacetic acid
- KM:
-
Kao’s medium
- KN:
-
Kinetin
- MES:
-
2-(N-Morpholino) ethane-sulfonic acid
- MS:
-
Murashige and Skoog’s medium
- N6:
-
Chu’s (N6) medium
- NAA:
-
α-Naphthalene acetic acid
- TDZ:
-
N-Phenyl-N′-1,2,3-thiadiazol-5-ylurea
References
Chase AR (1997) Foliage plant diseases: diagnosis and control. American Phytopathological Society, St Paul
Chen J, McConnell DB, Henny RJ, Norman DJ (2005) The foliage plant industry. Hortic Rev 31:47–112
Chu CC, Wang CC, Sun CS, Hus C, Yin KC, Chu CY, Bi FY (1975) Establishment of an efficient medium for anther culture of rice through comparative experiments on the nitrogen sources. Sci Sin 18:659–668
Das P, Palai SK, Patra A, Samantaray S, Rout GR (1999) In vitro somatic embryogenesis in Typhonium trilobatum Schott. Plant Growth Regul 27:193–197
Fiore S, Pasquale FD, Carimi F, Maurizio S (2002) Effect of 2,4-D and 4-CPPU on somatic embryogenesis from stigma and style transverse thin cell layer of Citrus. Plant Cell Tissue Organ Cult 68:57–63
Gomez L, Valverde R, Arias O, Thorpe T (1992) Regeneration of Xanthosoma sagittifolium by somatic embryogenesis. Agron Costarricense 16:219–223
Henny RJ, Chen J (2003) Foliage plant cultivar development. Plant Breed Rev 23:245–290
Hosoki T (1975) Propagation of tropical plants by tissue culture. Ph.D. Thesis, University of Hawaii, Honolulu
Huxley A (1994) The new royal horticultural society dictionary of gardening. Macmillan, London
Kao KN (1977) Chromosomal behavior in somatic hybrids of soybean-Nicotiana glauca. Mol Gen Genet 150:225–230
Kuehnle AR, Chen FC, Sugii N (1992) Somatic embryogenesis and plant regeneration in Anthurium andraeanum hybrids. Plant Cell Rep 11:438–442
Matsumoto TK, Kuehnle AR (1997) Micropropagation of Anthurium. In: Bajaj YPS (ed) Biotechnology in agriculture and forestry, vol 40. High-tech and micropropagation VI. Springer, Berlin Heidelberg New York, pp 14–29
Matsumoto TK, Webb DT, Kuehnle AR (1996) Histology and origin of somatic embryos derived from Anthurium andraeanum Linden ex Andre lamina. J Am Soc Hortic Sci 121:404–407
Mayo SJ, Bogner J, Boyce PC (1997) The genera of Araceae. Royal Botanical Gardens, Kew
Merkle SA (1997) Somatic embryogenesis in ornamentals. In: Geneve RL, Preece JE, Merkle SA (eds) Biotechnology of ornamental plants. CAB International, Wallingford, pp 13–33
Millan-Mendoza BM (1998) Regeneration of Rubus in vitro using forchlorfenuron (CPPU). Rev Fac Agron (LUZ) 15:242–248
Miller LR (1976) Tissue culture propagation of some tropical foliage plants. M.Sc. Thesis, University of California, Riverside, Calif.
Murthy BNS, Saxena PK (1994) Somatic embryogenesis in peanut (Arachis hypogaea L.): stimulation of direct differentiation of somatic embryos by forchlorfenuron (CPPU). Plant Cell Rep 14:145–150
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–479
Nakajima I, Kobayashi S, Nakamura Y (2000) Embryogenic callus induction and plant regeneration from unfertilized ovule of ‘Kyoho’ grape. J Jpn Soc Hortic Sci 69:186–188
Norman DJ, Yuen JMF (1998) A distinct pathotype of Ralstonia (Pseudomonas) solanacearum race 1, biovar 1 entering Florida in pothos (Epipremnum aureum) cuttings. Can J Plant Pathol 20:171–175
Qu L, Chen J, Henny RJ, Huang YF, Caldwell RD, Robinson CA (2000) Thidiazuron promotes adventitious shoot regeneration from pothos (Epipremnum Aureum) leaf and petiole explants. In Vitro Cell Dev Biol Plant 38:268–271
Rani V, Raina SN (2000) Genetic fidelity of organized meristem-derived micropropagated plants: a critical reappraisal. In Vitro Cell Dev Biol Plant 36:319–330
Reynolds AG, Nardle DA, Zuruwski C, Looney NE (1992) Phenylureas CPPU and thidiazuron affect yield components, fruit composition and storage potential of four seedless grape selections. J Am Soc Hortic Sci 117:85–89
Rubino L, Russo M (1997) Molecular analysis of the pothos latent virus genome. J Gen Virol 78:1219–1226
Smith CN, Scarborough EF (1981) Status and development of foliage plant industries. In: Joiner J (ed) Foliage plant production. Prentice-Hall, Englewood Cliffs, pp 1–39
Tsuro M, Koda M, Inoue M (1999) Comparative effect of different type of cytokinin for shoot formation and plant regeneration in leaf-derived callus of lavender (Lavandula vera DC). Sci Hortic 81:331–336
USDA (1999) 1998 census of horticultural specialties. United States Department of Agriculture, Washington
Werbrouck SPO, Eeckhaut TGR, Debergh PC (2000) Induction and conversion of somatic embryogenesis on the anther filament of Spathiphyllum schott. Acta Hortic 520:263–269
Wick RL, Dicklow MB (2002) Epipremnum, a new host for Phytophthora capsici. Plant Dis 86:1050
Wright ER, Grijalba PE, Gasoni L (2001) First report of Rhizoctonia solani AG-4 on Epipremnum aureum in Buenos Aires, Argentina. Plant Dis 85:96
Wu AM, Zhu BC, Li QY (1996) Studies on somatic embryogenesis of Pinellia pedatisecta Schott. Acta Agron Sin 22:66–71
Acknowledgements
We thank Dr. Dennis J. Gray for providing photographic equipment used in this study and for his valuable comments on this manuscript’s revision, and Kelly Everitt for critical reading of this manuscript. This research was supported in part by the Gloeckner Foundation, Inc., New York and USDA-TSTAR, and approved for publication as journal series R-10158 by the Florida Agricultural Experiment Station.
Author information
Authors and Affiliations
Corresponding author
Additional information
Communicated by K.K. Kamo
Rights and permissions
About this article
Cite this article
Zhang, Q., Chen, J. & Henny, R.J. Direct somatic embryogenesis and plant regeneration from leaf, petiole, and stem explants of Golden Pothos. Plant Cell Rep 23, 587–595 (2005). https://doi.org/10.1007/s00299-004-0882-z
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00299-004-0882-z