Abstract
Key message
Cryopreservation provides valuable genes for further breeding of elite cultivars, and cryotherapy improves the production of virus-free plants in Malus spp., thus assisting the sustainable development of the apple industry.
Abstract
Apple (Malus spp.) is one of the most economically important temperate fruit crops. Wild Malus genetic resources and existing cultivars provide valuable genes for breeding new elite cultivars and rootstocks through traditional and biotechnological breeding programs. These valuable genes include those resistant to abiotic factors such as drought and salinity, and to biotic factors such as fungi, bacteria and aphids. Over the last three decades, great progress has been made in apple cryobiology, making Malus one of the most extensively studied plant genera with respect to cryopreservation. Explants such as pollen, seeds, in vivo dormant buds, and in vitro shoot tips have all been successfully cryopreserved, and large Malus cryobanks have been established. Cryotherapy has been used for virus eradication, to obtain virus-free apple plants. Cryopreservation provided valuable genes for further breeding of elite cultivars, and cryotherapy improved the production of virus-free plants in Malus spp., thus assisting the sustainable development of the apple industry. This review provides updated and comprehensive information on the development and progress of apple cryopreservation and cryotherapy. Future research will reveal new applications and uses for apple cryopreservation and cryotherapy.
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Abbreviations
- AD:
-
Apical dome
- ALCSV:
-
Apple leaf chlorotic spot virus
- ApMV:
-
Apple mosaic virus
- ASGV:
-
Apple stem grooving virus
- ASPV:
-
Apple stem pitting virus
- BA:
-
N6-benzyladenine
- DMSO:
-
Dimethyl sulfoxide
- FWB:
-
Fresh weight basis
- GA3 :
-
Gibberellic acid 3
- IBA:
-
Indole-3-butyric acid
- ISSR:
-
Inter-simple sequence repeat
- JKI:
-
Julius Kühn Institute
- LN:
-
Liquid nitrogen
- LNV:
-
Liquid nitrogen vapor
- LP:
-
Leaf primordium
- MS:
-
Murashige and Skoog
- NLGRP:
-
National Laboratory for Genetic Resources Preservation
- PVS:
-
Plant vitrification solution
- PVS2:
-
Plant vitrification solution 2
- PVS3:
-
Plant vitrification solution 3
- RAPD:
-
Random amplified polymorphic DNA
- RBDV:
-
Raspberry bushy dwarf virus
- RH:
-
Relative humidity
- TTC:
-
2, 3, 5-triphenyl tetrazolium chloride solution
- USDA:
-
United States Department of Agriculture
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Acknowledgements
The authors appreciate the use of published data from Monika Höfer, Julius Kühn Institute, Dresden, Germany. They also thank Jean Carlos Bettoni for providing an internal review of the manuscript. Financial support was received from the Department of Science and Technology of Shaanxi Province, China (2014KTCL02-05) (Q. Wang) and USDA Agricultural Research Service in-house appropriated funds Project Number 3012-21000-014-00D (G. Volk).
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Wang, MR., Chen, L., Teixeira da Silva, J.A. et al. Cryobiotechnology of apple (Malus spp.): development, progress and future prospects. Plant Cell Rep 37, 689–709 (2018). https://doi.org/10.1007/s00299-018-2249-x
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DOI: https://doi.org/10.1007/s00299-018-2249-x