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The transcriptional response to salicylic acid plays a role in Fusarium yellows resistance in Brassica rapa L.

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Abstract

Key message

Fusarium yellows resistant and susceptible lines in Brassica rapa showed different salicylic acid responses; the resistant line showed a similar response to previous reports, but the susceptible line differed.

Abstract

Fusarium yellows caused by Fusarium oxysporum f. sp. conglutinans (Foc) is an important disease. Previous studies showed that genes related to salicylic acid (SA) response were more highly induced following Foc infection in Brassica rapa Fusarium yellows resistant lines than susceptible lines. However, SA-induced genes have not been identified at the whole genome level and it was unclear whether they were up-regulated by Foc inoculation. Transcriptome analysis with and without SA treatment in the B. rapa Fusarium yellows susceptible line ‘Misugi’ and the resistant line ‘Nanane’ was performed to obtain insights into the relationship between SA sensitivity/response and Fusarium yellows resistance. ‘Nanane’s up-regulated genes were related to SA response and down-regulated genes were related to jasmonic acid (JA) or ethylene (ET) response, but differentially expressed genes in ‘Misugi’ were not. This result suggests that Fusarium yellows resistant and susceptible lines have a different SA response and that an antagonistic transcription between SA and JA/ET responses was found only in a Fusarium yellows resistant line. SA-responsive genes were induced by Foc inoculation in Fusarium yellows resistant (RJKB-T23) and susceptible lines (RJKB-T24). By contrast, 39 SA-induced genes specific to RJKB-T23 might function in the defense response to Foc. In this study, SA-induced genes were identified at the whole genome level, and the possibility, the defense response to Foc observed in a resistant line could be mediated by SA-induced genes, is suggested. These results will be useful for future research concerning the SA importance in Foc or other diseases resistance in B. rapa.

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Availability of data

The RNA-sequencing data have been deposited with DDBJ (https://www.ddbj.nig.ac.jp) under DRA010766.

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Acknowledgements

Computations were partially performed on the NIG supercomputer at ROIS National Institute of Genetics.

Funding

This work was funded by Grant-in-Aid for JSPS Research Fellow to NM and grants from Project of the NARO Bio-oriented Technology Research Advancement Institution (Research program on development of innovation technology) to RF.

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Authors and Affiliations

  1. Graduate School of Agricultural Science, Kobe University, Rokkodai, Nada-ku, Kobe, 657-8501, Japan

    Naomi Miyaji, Takeshi Takasaki-Yasuda & Ryo Fujimoto

  2. Iwate Biotechnology Research Center, Narita,, Kitakami, Iwate, 024-0003, Japan

    Motoki Shimizu

  3. CSIRO Agriculture and Food, Canberra, ACT 2601, Australia

    Elizabeth S. Dennis

  4. University of Technology, Sydney, Broadway, PO Box 123, Ultimo, NSW, 2007, Australia

    Elizabeth S. Dennis

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  1. Naomi Miyaji
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  2. Motoki Shimizu
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  3. Takeshi Takasaki-Yasuda
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  4. Elizabeth S. Dennis
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  5. Ryo Fujimoto
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Contributions

RF, ESD, and TTY designed research. NM performed research. NM and MS analyzed the RNA-seq data. NM, RF, and ESD wrote the paper.

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Correspondence to Ryo Fujimoto.

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Communicated by Kinya Toriyama.

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Miyaji, N., Shimizu, M., Takasaki-Yasuda, T. et al. The transcriptional response to salicylic acid plays a role in Fusarium yellows resistance in Brassica rapa L.. Plant Cell Rep 40, 605–619 (2021). https://doi.org/10.1007/s00299-020-02658-1

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