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The influence of isoflavone for denervation-induced muscle atrophy

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Abstract

Purpose

Decrease in activity stress induces skeletal muscle atrophy. A previous study showed that treatment with a high level (20%) of isoflavone inhibits muscle atrophy after short-term denervation (at 4 days) in mice. The present study was designed to elucidate whether the dietary isoflavone aglycone (AglyMax) at a 0.6% prevents denervation-mediated muscle atrophy, based on the modulation of atrogin-1- or apoptosis-dependent signaling.

Methods

Mice were fed either a normal diet or 0.6% AglyMax diet. One week later, the right sciatic nerve was cut. The wet weight, mean fiber area, amount of atrogin-1 and cleaved caspase-3 proteins, and the percentages of apoptotic nuclei were examined in the gastrocnemius muscle at 14 days after denervation.

Results

The 0.6% AglyMax diet significantly attenuated denervation-induced decreases in fiber atrophy but not the muscle wet weight. In addition, dietary isoflavone suppressed the denervation-induced apoptosis in spite of there being no significant changes in the amount of cleaved caspase-3 protein. In contrast, the 0.6% AglyMax diet did not significantly modulate the protein expression of atrogin-1 in the denervated muscle of mice.

Conclusions

The isoflavone aglycone (AglyMax) at a 0.6% significantly would modulate muscle atrophy after denervation in mice, probably due to the decrease in apoptosis-dependent signaling.

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Acknowledgements

This work was supported by a research Grant-in-Aid for Scientific Research C (No. 17K01755) from the Ministry of Education, Culture, Sports, Science and Technology of Japan.

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Authors and Affiliations

  1. Research Center for Physical Fitness, Sports and Health, Toyohashi University of Technology, 1-1 Hibarigaoka, Tenpaku-cho, Toyohashi, 441-8580, Japan

    Shinpei Tabata, Miki Aizawa, Masakazu Kinoshita, Yoshinori Ito, Yusuke Kawamura & Kunihiro Sakuma

  2. Institute for Liberal Arts, Environment and Society, Tokyo Institute of Technology, 2-12-1 Ookayama, Meguro-ku, Tokyo, 152-8550, Japan

    Kunihiro Sakuma

  3. Nichimo Biotics Company, Tokyo, 140-0002, Japan

    Minoru Takebe & Weijun Pan

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  1. Shinpei Tabata
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Correspondence to Kunihiro Sakuma.

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Kunihiro Sakuma and all the co-authors declare that they have no conflict of interest.

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Tabata, S., Aizawa, M., Kinoshita, M. et al. The influence of isoflavone for denervation-induced muscle atrophy. Eur J Nutr 58, 291–300 (2019). https://doi.org/10.1007/s00394-017-1593-x

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  • DOI: https://doi.org/10.1007/s00394-017-1593-x

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