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Orientational screening of ssDNA-templated silver nanoclusters and application for bleomycin assay

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Abstract

Orientational screening is a recently proposed strategy for the selection of DNA templates for silver nanoclusters (AgNCs). The feasibility of this strategy is facilitated by the accumulated experience of synthesizing DNA-templated AgNCs. Herein, a single-stranded DNA template of endowing AgNCs with high brightness (QY = 27.99%) was obtained through an orientational screening process. It was also found that double-stranded DNA rich in GC base pairs could also serve as synthesizing template for AgNCs. A facile method was then developed for the facile assay of bleomycin that might be added in illegally pronouncing products due to its low price and easy accessibility. The detection limit of this method was as low as 4.27 nM, with high sensitivity and good selectivity.

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Funding

This work was supported by the National Key Research and Development Program of China (No. 2017YFC1601704).

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Authors and Affiliations

  1. State Key Laboratory of Food Science and Technology, National Centre for Technology Innovation On Fast Biological Detection of Grain Quality and Safety, Jiangnan University, Wuxi, China

    Yahui Guo, Xinyue Pan, Yuliang Cheng, Hang Yu, Yunfei Xie, Fangwei Yang, Weirong Yao & He Qian

  2. School of Food Science and Technology, Jiangnan University, Wuxi, China

    Yahui Guo, Xinyue Pan, Yuliang Cheng, Hang Yu, Yunfei Xie, Fangwei Yang, Hongwei Zhang, Weirong Yao & He Qian

  3. Technology Center of Qingdao Customs District, Qingdao, China

    Hongwei Zhang

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  1. Yahui Guo
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  4. Hang Yu
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Guo, Y., Pan, X., Cheng, Y. et al. Orientational screening of ssDNA-templated silver nanoclusters and application for bleomycin assay. Colloid Polym Sci 299, 1643–1649 (2021). https://doi.org/10.1007/s00396-021-04890-x

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  • DOI: https://doi.org/10.1007/s00396-021-04890-x

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