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Molecular cytogenetic characterization of novel wheat-Thinopyrum bessarabicum recombinant lines carrying intercalary translocations

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Abstract

Thinopyrum bessarabicum (2n = 2x = 14, JJ or EbEb) is a valuable source of genes for bread wheat (2n = 6x = 42) improvement because of its salinity tolerance and disease resistance. Development of wheat-Th. bessarabicum translocation lines by backcrossing the amphiploid in the absence of the Ph1 gene (allowing intergenomic recombination) can assist its utilization in wheat improvement. In this study, six novel wheat-Th. bessarabicum translocation lines involving different chromosome segments (T4BS.4BL-4JL, T6BS.6BL-6JL, T5AS.5AL-5JL, T5DL.5DS-5JS, T2BS.2BL-2JL, and the whole arm translocation T1JS.1AL) were identified and characterized using genomic in situ hybridization (GISH) and fluorescent in situ hybridization (FISH). No background translocations between wheat genomes were observed. The involvement of five of the seven chromosomes and small terminal segments of Th. bessarabicum chromosome arm were important, contributing to both reduced linkage drag of the derived lines by minimizing agronomically deleterious genes from the alien species and high stability including transmission of the alien segment. All three wheat genomes were involved in the translocations with the alien chromosome, and GISH showed the Th. bessarabicum genome was more closely related to the D genome in wheat. All the introgression lines were disomic, stable, and with good morphological characters.

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Acknowledgments

We would like to thank “Monsanto Beachell-Borlaug International Fellowship” (MBBISP) program for funding the PhD work of Chetan Patokar and the EU Marie Curie fellowship (FP7-PEOPLE-2013-IEF 625835) to Adél Sepsi. The IAEA-FAO Cooperative Research Project “Climate Proofing of Food Crops: Genetic Improvement for Adaptation to High Temperatures in Drought Prone Areas and Beyond,” D2.30.29, is acknowledged by Trude Schwarzacher and J.S. Heslop-Harrison.

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The authors declare that they have no competing interests.

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  1. Department of Biology, University of Leicester, Leicester, LE1 7RH, UK

    Chetan Patokar, Adel Sepsi, Trude Schwarzacher & J. S. Heslop-Harrison

  2. International Maize and Wheat Improvement Center (CIMMYT), Km. 45, Carretera, México-Veracruz El Batán, CP 56237, Texcoco, Edo. de México, Mexico

    Masahiro Kishii

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  1. Chetan Patokar
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  2. Adel Sepsi
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Correspondence to Chetan Patokar.

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Patokar, C., Sepsi, A., Schwarzacher, T. et al. Molecular cytogenetic characterization of novel wheat-Thinopyrum bessarabicum recombinant lines carrying intercalary translocations. Chromosoma 125, 163–172 (2016). https://doi.org/10.1007/s00412-015-0537-6

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