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The effect of post-resistance exercise alcohol ingestion on lipopolysaccharide-stimulated cytokines

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Abstract

Purpose

To examine the effect of post-resistance exercise alcohol ingestion on lipopolysaccharide (LPS)-stimulated production of IFNγ, TNF-α, IL-1β, IL-6, IL-8, and IL-10.

Methods

Recreationally resistance-trained men (n = 10, 25 ± 3 year, 177 ± 7 cm, 83.8 ± 15.7 kg, 14.8 ± 8.5 % body fat) and women (n = 8, 23 ± 2 year, 161 ± 3 cm, 59.5 ± 6.0 kg, 26.5 ± 3.0 % body fat) completed two identical heavy back squat sessions (6 × 10 at 80 % 1 repetition maximum) followed by ingestion of either an alcohol (ALC; 1.09 g ethanol ·  kg fat-free mass−1) or water (PLA) drink. Blood samples were collected before exercise (PRE), and at 3 h (3 h), and 5 h (5 h) after exercise, stimulated with LPS, and analyzed for IFNγ, TNF-α, IL-1β, IL-6, IL-8, and IL-10 concentrations.

Results

There were no drink conditions by time effects for IFNγ, TNF-α, IL-1β, or IL-10. Regardless of condition, resistance exercise induce an increase in IFNγ, TNF-α, and IL-1β at 5 h compared to PRE but a decrease in IL-10 at 3 and 5 h compared to PRE. For ALC, IL-8 was reduced at 5 h compared to PLA. From PRE to 3 h, IL-6 was reduced for ALC but increased for PLA; resistance exercise induced an increase in IL-6 for both conditions at 5 h.

Conclusions

Heavy resistance exercise increased production of IFNγ, TNF-α, IL-1β, and Il-6 and decreased production of IL-10. Alcohol ingestion after resistance exercise affected aspects of inflammatory capacity (IL-6 and IL-8 production). It appears that some of the effects previously observed for alcohol ingestion alone on the LPS-stimulated cytokine production were overwhelmed by the response to resistance exercise.

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Abbreviations

1RM:

One-repetition maximum

3 h:

Three hours post-exercise time point

5 h:

Five hours post-exercise time point

ALC:

Alcohol condition

ANOVA:

Analysis of variance

BAC:

Blood alcohol concentration

IFN:

Interferon

IL:

Interleukin

LPS:

Lipopolysaccharide

PLA:

Placebo condition

PRE:

Pre-exercise time point

TNF:

Tumor necrosis factor

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Authors and Affiliations

  1. Applied Physiology Laboratory, Department of Kinesiology, Health Promotion, and Recreation, University of North Texas, 1155 Union Circle #310769, Denton, TX, 76203-5017, USA

    Danielle E. Levitt, Anthony A. Duplanty, Ronald G. Budnar Jr., Hui-Ying Luk, Alexander Fernandez, Travis J. Layman, Daniel L. Fancher, David W. Hill, Brian K. McFarlin & Jakob L. Vingren

  2. Department of Biological Sciences, University of North Texas, 1155 Union Circle #305220, Denton, TX, 76203-5017, USA

    Danielle E. Levitt, Anthony A. Duplanty, Ronald G. Budnar Jr., Hui-Ying Luk, Brian K. McFarlin & Jakob L. Vingren

Authors
  1. Danielle E. Levitt
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  2. Anthony A. Duplanty
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  3. Ronald G. Budnar Jr.
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  4. Hui-Ying Luk
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  5. Alexander Fernandez
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  6. Travis J. Layman
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  7. Daniel L. Fancher
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  8. David W. Hill
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  9. Brian K. McFarlin
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  10. Jakob L. Vingren
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Corresponding author

Correspondence to Jakob L. Vingren.

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Conflict of interest

The authors declare that they have no conflicts of interest.

Funding

This project was funded in part by grants from the American College of Sports Medicine—Texas Chapter and the National Strength and Conditioning Association.

Ethical approval

All procedures performed in this study involving human participants were in accordance with the ethical standards of the Institutional Review Board of the University of North Texas and in accordance with the 1964 Helsinki declaration and its later amendments. This article does not contain any studies with animals performed by the authors.

Informed consent

Informed consent was obtained from all individual participants included in the study.

Additional information

Communicated by William J. Kraemer.

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Levitt, D.E., Duplanty, A.A., Budnar, R.G. et al. The effect of post-resistance exercise alcohol ingestion on lipopolysaccharide-stimulated cytokines. Eur J Appl Physiol 116, 311–318 (2016). https://doi.org/10.1007/s00421-015-3278-6

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  • DOI: https://doi.org/10.1007/s00421-015-3278-6

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